LOCUS       sequence-30715-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus.
  JOURNAL   Pflugers Arch. 2007 Apr . 454(1):155-63.
  PUBMED    17146677
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers 
            Arch. 2007 Apr . 454(1):155-63."
COMMENT     Sequence Label: pUNIV-EGFP
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      186..641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(273..292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     483..504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(781..801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat ccgggctggc
      121 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      181 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      601 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
      661 cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg
      721 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
      781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      841 aaagcatgca tctcaattag tcagcaacc
//
