LOCUS       sequence-307152-       12662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  suicide vector for knock in into the B. burgdorferi chromosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 12662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pKIKan_idCas9_Chr_center
FEATURES             Location/Qualifiers
     source          1..12662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             426..437
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(2115..2129,2130..2135,2136..2152,2153..2159)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2135..2151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2167..2183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2202..2213
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2220..2246,2247..2249,2250..4736,4737..4739,
                     4740..6326)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6378..7187)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7101..7120
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(9668..9687)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9669..9687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9694..9711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9694..9710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9703..9725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9851..10209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9938..9957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10148..10169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      10285..10873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10774..10793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11027..11044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11161..11182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11197..11202,11203..11220,11221..11227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11235..11251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11259..11275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11259..11275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caattttaaa gcttaataat aattttttaa tgcattattc tacagaatca aaatttaatt
       61 tttaatgtat gaagcaaaaa ttaagttgga ttttattatt ttgttttttg tcttgtagat
      121 ctgaatctag attggctgaa aatgttttaa tagagttttt tgattctatt aaaaattttc
      181 aaagcagtcc tgaaatattt tttaattatt taaatattcc aagtgatgat gatctgaagg
      241 caaaaattcg tgggttgaaa tctcaggcaa aggatgattt cattttttat cctttgtttt
      301 ttaataatct aagatatgag ataataggta gaaaaaatat ttctaagggc tttgaatttg
      361 aagttgttat taaaaatatt aactttcaaa acggtataga aaaatttttg gctaaattaa
      421 ataaaattga agggagatct ttaaatatta aaaatttaga aaaaaaagag cgtaaaaaaa
      481 tatttgacaa tttaataaat gaagttattg gagagttgga tgattttgat tacactgaag
      541 ttgttcattt ttttagagta gttaagagtt cttctgaaag ttataaaata gagcttttag
      601 gagatgtttt aaatatacag tctagaaata agcttattaa tgatcttttt ttggttttat
      661 cgcctggaat ttaaaatagt gactagtaaa taatgcccgt gtccctgttg aatagggaca
      721 cggtttttta acaatttttt tattcctagg ccttagtggc cggtacccgg ggatccttat
      781 tactggccgc tttctagcct ggatacttgt ctggctagtt gcattagaga gtcagctaga
      841 gccctagggc tagctgtttg tgtattcgga gccagtgtag tttttctttt tactaacgat
      901 acgggtaata attgattgcc ttttacagcc tgaccttgag acaattgcaa aagtctgtcc
      961 acagaggttt gacctaataa tctaaaatct tgtttgatag tagtaagtgg aggtatgtag
     1021 caactagaat cttctgtatc atcataaccc acaactgaaa tatcagcccc aactctaagt
     1081 ccactttcag taatagctcg catagctcct aaagccattt gatcattagc aactaacata
     1141 gcagtaggga caatgccttc atttaacatt tgcattgttt gctgaaatcc tgacatagca
     1201 gaccaatctc cttctctttc tgctattggt tgtatttgat ttcttgttaa atacttgtgc
     1261 catcctgcta atctcaatct ggcacttact gaacttaagg gacctgcaag tagtgcaatt
     1321 tgttgatggc ctaatgcaac aaggtgttca actcctaacc ttgttccatc ttcgtgagaa
     1381 aaaattatag agtttattgg ggtttggtcc gacacatcca aaaacaatgc tggaacatta
     1441 gtacatgctg cttcaactgc aatcgcatct tggtcatcaa gaggataatt aattataagt
     1501 ccactaacac gttgagctaa taaattatgt actgctgcct tacaagcctc tacacctgat
     1561 ctctcaacca ttgatacaac aacgcttgca ccaagttgat cagctctcga tttaattgcg
     1621 gctacaattt gactaggcgc atgtaatgca agtgatgatg tggcaactcc aattaaaagt
     1681 gattgtttcc ccgcaagttg ctgagcaacg cgatttggta tataattcaa ctctgccata
     1741 gctgcttcaa ccttctctct tgtttttgca gatacatgac ttgcttggtt cacaactctc
     1801 gaaaccgttt ggtagctaac cccggcatat tcagcaacat catacaaagt aacaggtttc
     1861 atggatcatt cctccatgat aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt
     1921 taaaaagaat agcacaaaaa ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat
     1981 aattattatt atttatttct ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct
     2041 ttcatgcgct taacggtaaa atccaaggta ttaagttcac cttccacttt tctctctcta
     2101 tcttctctag aaaatcataa aaaatttatt tgctttgtga gcggataaca attataatag
     2161 attcaattgt gagcggataa caatttcaca cagaattcat taaagaggag aaattacata
     2221 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
     2281 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
     2341 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
     2401 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
     2461 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
     2521 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
     2581 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
     2641 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
     2701 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
     2761 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
     2821 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
     2881 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
     2941 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
     3001 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
     3061 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     3121 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
     3181 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     3241 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     3301 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     3361 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
     3421 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     3481 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     3541 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
     3601 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     3661 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     3721 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     3781 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
     3841 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     3901 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     3961 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     4021 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     4081 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     4141 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     4201 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     4261 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     4321 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     4381 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     4441 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     4501 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     4561 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     4621 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     4681 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
     4741 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     4801 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     4861 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     4921 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     4981 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     5041 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     5101 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     5161 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     5221 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     5281 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     5341 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     5401 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     5461 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc
     5521 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     5581 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     5641 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
     5701 aagagttact agggatcaca attctggaaa gaagttcctt tgaaaaaaat ccgattgact
     5761 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
     5821 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
     5881 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
     5941 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
     6001 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
     6061 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
     6121 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
     6181 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
     6241 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
     6301 atttgagtca gctaggaggt gactaacccg ggtaaaaaaa caaaagatcc tttaaaggat
     6361 cttttgttaa taccgggtta gaaaaactca tcgagcatca aatgaaactg caatttattc
     6421 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     6481 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     6541 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     6601 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     6661 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     6721 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     6781 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     6841 tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg
     6901 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     6961 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     7021 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc
     7081 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     7141 ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggcccatatg gaaacctccc
     7201 tcatttaaaa ttgcttttaa ctaaattata taataattca aatctttttg ttcgttaatt
     7261 tgtatttttt tattaacata gtccaagttt atttcaaatt ttttcaactt actgccaggc
     7321 acttcaaaaa aaagatctgc aagcactatt tccataacac cgtgaagtct tctggcacca
     7381 agattttcac tctcaagatt catattaaaa gtaagctctg caattctatc tatagcttcc
     7441 tcactaaact ttaaatctaa atcataaacc ttaaacatcg caacatattg ttttattaaa
     7501 gaattttttg tttgttttaa aatttttttc aaatcgtcta tgcttaagct cttaagttca
     7561 accttaatag gaaatcttcc ttgaaccgcg gaagggcgaa ttctgcagct agtttaaaat
     7621 ttatttatct tgattattat tttttatgga gttctctttt attgcaaatt cttttattct
     7681 ttttgcaagt tgcacgttta cttttatttt ttctgaaatt tcgttttcac ttaaaggcaa
     7741 tatatcttta taggttccaa ttgattttaa tattttttgg gctgtttttt ctccaattcc
     7801 gtgtattttt gtatacaata gggttatttt ttctcttctt tttttgttaa atccgttggc
     7861 ctttctgtgt gcttcgtctc ttacattttg cagtattcta agagcaggat gtccttgggg
     7921 tagatttatt ccttttttgt tagttgttaa gaatattgtt tcttgttttt ttgccaacga
     7981 gcagacttta actttgtttt ctatttttaa gccctttaag atagaaagag cggcatttaa
     8041 ttgtcctttg cccccgtcaa ttaaaattaa attcggtagt tctaagttgt tattaattat
     8101 ttctgaatat cttcttgata ttacttcttt tattgcctta aagtcgtcaa tttctccttt
     8161 taatagtgaa tttagtttgt aaagcctgta gttttcttta aaaggcattc ccattttaaa
     8221 agtaaccata gaagctactg tttcttgacc tttaagatga gcaatgtcaa atccttcaat
     8281 tattttggga agtttgtcca tttctaaaac aattttcaaa ctttcaagtg ctttggtgct
     8341 tttattctca tattctctta aagataattc agcattagat atggccattt ccattatttt
     8401 taaaatttct tctgtttctt tgtaaataat ttctgttttt gtatttttaa tttcatttat
     8461 tagtttttca acatttttag tgtcgatatc tttgagaaaa atatgaattt tgtctggtac
     8521 tatcatatta atagatgtgt aatattgaat caaaaattgt aaaatcagct cattttcttt
     8581 gcatatactc tcatcaaagt ttgcatctct ttcaactaat tttccatttc tatattttaa
     8641 tactattatt gtatttacat tttctcctgg atgaacatgg acataatcta tgtttaaatt
     8701 attggttttt gtaacgattt ggatttgatt aatttctatt aaagaatttc taatttcttt
     8761 taatttgata gcggtttcaa aatcttcttt ttgtatggca tgttttaatt tgatatcaat
     8821 ttgacttgat atttcggata tatttccatt tagtatggat tttgccttat ctagctcttt
     8881 ttgatattct ttttcaaggt tttccttgta gcatactcca aggcactgtc ccatatggta
     8941 atataggcaa ggagcattgg attttttttt acactttcta atcttaaatg ttttgttaat
     9001 aaaatctagt acttgatcta atttttttac attggtaaat ggtccaaaat attcgctttt
     9061 gtcattaatt atttttctgg ttttgaaaat tcttggatat ttttcatggg ttattctcac
     9121 catggggtaa ccttttccat cttttaattt tacattgtaa tcaggtttgt gggttttaat
     9181 tagattgcac tctagaagca atgcttcgta ttcgctattt gttgtaataa cttctattga
     9241 ttttacattt ttcattaata ttttgatttt gtgactattt ttttctaaaa aataactttt
     9301 tactcttgat cttagatttt ttgcttttcc aatatagagt atttttttat tttcatttag
     9361 catcttatag caaccgcttg tggttggtaa ttttattact ttttcgaata aatttgttag
     9421 gttctctttc ataatgttag gcagttttta gattttaaaa atcatcttat gatataatat
     9481 tgtatcataa ggcagtttgt tttatgtttt gaggtattta aaaatatggg taataacaaa
     9541 taaaaggtca aaaatgagat taattttaat gctcttgcta tcttttttgt gcttttctac
     9601 tctgttatct caagaattga agttgatact tgattcgctc gagcatgcat ctagagggcc
     9661 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     9721 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     9781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     9841 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    10081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    10201 tttgatttac atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11041 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11101 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11221 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    11281 ttacgccaag cttggtaccg agctcctaag taatgcaaat aataatccca cattgtatgg
    11341 cttgtatgaa ctattggtat gttgtgtttt aatgcaattt gttttccaat ttttcccata
    11401 gaaaattcag agtgagtatg aatgatgtct ggtttatagc tttgtattat tttggatatt
    11461 tttcttttat tgggaaaagc tattacagca tcaagttttt tatttatttg aatagatgag
    11521 catctgtaaa cgtttttttc gtttaaagat tttttggatt ttgggcaaaa tatgtaaacc
    11581 tcatagccat ttttttcaaa tccctcttta atttgtttta ttgatgttgc tactccattt
    11641 ttttctggga tatatgtatc tgtaaatatt gcgactttca tattctcctc ctagcttaag
    11701 tataatatat ttgctacttg gattataatt tactttaatg caagcggttt atttattgtt
    11761 gggtaatgag caaggtttaa aagaagccta tttaaaagag cttttaatta aaatggatgc
    11821 ttttaaatcc gaagtttcag ttactaaaat ttttttgtca gaactctcag ctgtaggatt
    11881 tgctgagaaa ttattttcca attctttttt ttcaaaaaaa gaaattttta ttgtttatga
    11941 gtctgaactt ttaaaagcag gaaaagattt agagctagtg tgtaattcaa tcttaaagtc
    12001 taacaataaa actgttattt ttgtttccaa tagcaataca tgtaacattg attttaagaa
    12061 taagcttaag tttataaaaa aagtttttta tgagattcct gatgatgata aatttacatt
    12121 tgtaaaaaga aattttttta atcttaatat taaaattaca gattctgcaa taaatttaat
    12181 gcttttaatg ttaaattcag atactaaaat tttgaaattt tatatagatt cttttgcgct
    12241 ttttgccaag aataatacca ttgaggagga agatatagct tcttggatta gttttattcg
    12301 ctttgagaac accttttcct tatttaattc aattttgaga aaagatatga ctcagtcttt
    12361 gatcaagatt aagtctattt tggaccaggg agaagatttg cttaatattt taatgagtct
    12421 tatttggcaa tttaaaagat tattaaaggt gcaaatagat tataatgcat atgggagctt
    12481 gcagagtgca ttgaataaaa ataaaatctt tttttcatta aataaaattt atagagtagg
    12541 aattaaaaat tattcaattc cagaaattaa aattgttttg aaaattttat ataagtttga
    12601 tttatatttg agaatttatt ctaaaaatat tcatcaaaat ttgtcatatt ttttaatatt
    12661 ct
//
