LOCUS       sequence-307159-       11283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding full length wildtype human OGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK OGT (Neo)
FEATURES             Location/Qualifiers
     source          1..11283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4158..4668
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4587..4605
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4723..4747
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7902..7926)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7957..8545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8010..8030)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8428..8439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8588..9087
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8623..8646)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9020..9039
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9101..9904
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9164..9183)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9774..9793
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9995..10228
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10300..10421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10337..10356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10391..10410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10461..10596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10616..10635)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10617..10635)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10645..10662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10645..10661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10654..10676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10803..11258
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10890..10909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11100..11121
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc
     4141 ttgatatcga attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     4201 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     4261 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     4321 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     4381 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     4441 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     4501 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     4561 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     4621 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg
     4681 gttcgtcgac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     4741 gcaggtttaa aggaaccaat tcagtcgact ggatccatgg cgtcttccgt gggcaacgtg
     4801 gccgacagca cagggttagc tgagttggca catcgagaat atcaggcagg agattttgag
     4861 gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac tggtgtgctt
     4921 ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc tcactttagc
     4981 actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt ggggaatgtg
     5041 tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc attgcgtctc
     5101 aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc agcgggtgac
     5161 atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga tttgtactgt
     5221 gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga agccaaggca
     5281 tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag taatcttggc
     5341 tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga aaaggctgtc
     5401 acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt gaaagaggca
     5461 cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag tccaaatcac
     5521 gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat agatctggca
     5581 atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc ttactgcaac
     5641 ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg ttataataca
     5701 gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa tatcaaacga
     5761 gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga agtcttccca
     5821 gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg aaaactgcag
     5881 gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc tgatgcctac
     5941 tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt gcagtgttat
     6001 acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct ggcttccatt
     6061 cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc tctgaaactt
     6121 aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat tgtctgtgat
     6181 tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga ccagttagag
     6241 aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct ttctcatggc
     6301 ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat taatgttctt
     6361 cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg gctgcgtgta
     6421 ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca gtctattcca
     6481 ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc agacgatggc
     6541 acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct ttctcagatt
     6601 ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat ccttgtaaat
     6661 atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc agctcctatt
     6721 caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga ttatattatc
     6781 actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa attggcttat
     6841 atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct gaagaaaaaa
     6901 gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt tctgaatggc
     6961 atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa gatgaagtgt
     7021 cctgatggag gagacaatgc agatagcagt aacacagctc ttaatatgcc tgttattcct
     7081 atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat tcaaataaca
     7141 attaatggat tcagtattag caatggactg gcaactactc agatcaacaa taaggctgca
     7201 actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta cgggttacca
     7261 gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc ttctactttg
     7321 cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct gttgcgtttt
     7381 ccagcagtag gagaacctaa tattcaacag tatgcacaaa acatgggcct gccccagaac
     7441 cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg ccagctggct
     7501 gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga tgtcctctgg
     7561 gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt tgcagcatcc
     7621 cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga atatgaagac
     7681 atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg caaagtctgg
     7741 aagcaaagaa tatctagccc tctgttcaac accaaacaat acacaatgga actagagcgg
     7801 ctctatctac agatgtggga gcattatgca gctggcaaca aacctgacca catgattaag
     7861 cctgttgaag tcactgagtc agcataactc gagatatcta gacccagctt tcttgtacaa
     7921 agtggttgat atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt
     7981 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8041 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8101 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8161 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8221 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8281 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8341 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     8401 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8461 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8521 atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggact agtgatctaa
     8581 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     8641 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     8701 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     8761 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     8821 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     8881 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     8941 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     9001 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     9061 tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca
     9121 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     9181 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     9241 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     9301 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     9361 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     9421 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     9481 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     9541 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     9601 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     9661 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     9721 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     9781 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     9841 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     9901 ctgaggggat ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
     9961 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
    10021 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    10081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10201 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    10261 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
    10321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
    10441 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    10501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10561 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
    10621 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
    10681 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    10741 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    10801 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10861 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    10921 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    10981 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11041 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11101 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11161 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11221 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    11281 gtg
//
