LOCUS       sequence-30716-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus.
  JOURNAL   Pflugers Arch. 2007 Apr . 454(1):155-63.
  PUBMED    17146677
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers 
            Arch. 2007 Apr . 454(1):155-63."
COMMENT     Sequence Label: pUNIV-EGFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          164..296
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     279..298
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(458..479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(719..738)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgactgttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
       61 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagtgactct
      121 cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      181 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      241 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      301 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
      361 tagcctcgag aattccgttt ttatttttaa ttttctttca aatacttcca ccatggtgag
      421 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      481 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      601 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      721 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      781 catcgagctg aagggcatcg acttcaagga ggac
//
