LOCUS       sequence-307162-        9391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping Beauty transposase fused to SP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Moudgil A, Wilkinson MN, Chen X, He J, Cammack AJ, Vasek MJ, Lagunas
            T Jr, Qi Z, Lalli MA, Guo C, Morris SA, Dougherty JD, Mitra RD
  TITLE     Self-Reporting Transposons Enable Simultaneous Readout of Gene 
            Expression and Transcription Factor Binding in Single Cells.
  JOURNAL   Cell. 2020 Aug 20;182(4):992-1008.e21. doi: 
            10.1016/j.cell.2020.06.037. Epub 2020 Jul 24.
  PUBMED    32710817
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.06.037"; journalName: "Cell"; date: 
            "2020-08-20- 20"; volume: "182"; issue: "4"; pages: "992-1008.e21"
COMMENT     Sequence Label: SP1-SB100X
FEATURES             Location/Qualifiers
     source          1..9391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        508..711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     756..775
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3317..4336
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="GKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRKY
                     KHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVKR
                     VLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHND
                     HRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDILK
                     QHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENLW
                     AELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY"
     primer_bind     complement(4374..4391)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4380..4604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4891..5565)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5566..5846)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     5986..6008
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6046..6064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6105..6608
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6832..6853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6972..6992)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7127..7143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7127..7143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7175..7181,7182..7199,7200..7205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7358..7375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7609..7628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8288..9079,9080..9148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8911..8930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      721 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      781 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      841 ggtggaattc accatgagcg accaagatca ctccatggat gaaatgacag ctgtggtgaa
      901 aattgaaaaa ggagttggtg gcaataatgg gggcaatggt aatggtggtg gtgccttttc
      961 acaggctcga agtagcagca caggcagtag cagcagcact ggaggaggag ggcaggagtc
     1021 ccagccatcc cctttggctc tgctggcagc aacttgcagc agaattgagt cacccaatga
     1081 gaacagcaac aactcccagg gcccgagtca gtcaggggga acaggtgagc ttgacctcac
     1141 agccacacaa ctttcacagg gtgccaatgg ctggcagatc atctcttcct cctctggggc
     1201 tacccctacc tcaaaggaac agagtggcag cagtaccaat ggcagcaatg gcagtgagtc
     1261 ttccaagaat cgcacagtct ctggtgggca gtatgttgtg gctgccgctc ccaacttaca
     1321 gaaccagcaa gttctgacag gactacctgg agtgatgcct aatattcagt atcaagtaat
     1381 cccacagttc cagaccgttg atgggcaaca gctgcagttt gctgccactg gggcccaagt
     1441 gcagcaggat ggttctggtc aaatacagat cataccaggt gcaaaccaac agattatcac
     1501 aaatcgagga agtggaggca acatcattgc tgctatgcca aacctactcc agcaggctgt
     1561 ccccctccaa ggcctggcta ataatgtact ctcaggacag actcagtatg tgaccaatgt
     1621 accagtggcc ctgaatggga acatcacctt gctacctgtc aacagcgttt ctgcagctac
     1681 cttgactccc agctctcagg cagtcacgat cagcagctct gggtcccagg agagtggctc
     1741 acagcctgtc acctcaggga ctaccatcag ttctgccagc ttggtatcat cacaagccag
     1801 ttccagctcc tttttcacca atgccaatag ctactcaact actactacca ccagcaacat
     1861 gggaattatg aactttacta ccagtggatc atcagggacc aactctcaag gccagacacc
     1921 ccagagggtc agtgggctac aggggtctga tgctctgaac atccagcaaa accagacatc
     1981 tggaggctca ttgcaagcag gccagcaaaa agaaggagag caaaaccagc agacacagca
     2041 gcaacaaatt cttatccagc ctcagctagt tcaaggggga caggccctcc aggccctcca
     2101 agcagcacca ttgtcagggc agacctttac aactcaagcc atctcccagg aaaccctcca
     2161 gaacctccag cttcaggctg ttccaaactc tggtcccatc atcatccgga caccaacagt
     2221 ggggcccaat ggacaggtca gttggcagac tctacagctg cagaacctcc aagttcagaa
     2281 cccacaagcc caaacaatca ccttagcccc aatgcagggt gtttccttgg ggcagaccag
     2341 cagcagcaac accactctca cacccattgc ctcagctgct tccattcctg ctggcacagt
     2401 cactgtgaat gctgctcaac tctcctccat gccaggcctc cagaccatta acctcagtgc
     2461 attgggtact tcaggaatcc aggtgcaccc aattcaaggc ctgccgttgg ctatagcaaa
     2521 tgccccaggt gatcatggag ctcagcttgg tctccatggg gctggtggtg atggaataca
     2581 tgatgacaca gcaggtggag aggaaggaga aaacagccca gatgcccaac cccaagccgg
     2641 tcggaggacc cggcgggaag catgcacctg cccctactgt aaagacagtg aaggaagggg
     2701 ctcgggggat cctggcaaaa agaaacagca tatttgccac atccaaggct gtgggaaagt
     2761 gtatggcaag acctctcacc tgcgggcaca cttgcgctgg catacaggcg agaggccatt
     2821 tatgtgtacc tggtcatact gtgggaaacg cttcacacgt tcggatgagc tacagaggca
     2881 caaacgtaca cacacaggtg agaagaaatt tgcctgccct gagtgtccta agcgcttcat
     2941 gaggagtgac cacctgtcaa aacatatcaa gacccaccag aataagaagg gaggcccagg
     3001 tgtagctctg agtgtgggca ctttgcccct ggacagtggg gcaggttcag aaggcagtgg
     3061 cactgccact ccttcagccc ttattaccac caatatggta gccatggagg ccatctgtcc
     3121 agagggcatt gcccgtcttg ccaacagtgg catcaacgtc atgcaggtgg cagatctgca
     3181 gtccattaat atcagtggca atggcttcaa gttgggcggc ggcgcccccg ccgtgggcgg
     3241 cggacccaag gccgcggata aaccaaaaaa gaaaagaaaa ctagcaggtg gcggaggagg
     3301 cggtgggggt ggcggtggaa aatcaaaaga aatcagccaa gacctcagaa aaagaattgt
     3361 agacctccac aagtctggtt catccttggg agcaatttcc aaacgcctgg cggtaccacg
     3421 ttcatctgta caaacaatag tacgcaagta taaacaccat gggaccacgc agccgtcata
     3481 ccgctcagga aggagacgcg ttctgtctcc tagagatgaa cgtactttgg tgcgaaaagt
     3541 gcaaatcaat cccagaacaa cagcaaagga ccttgtgaag atgctggagg aaacaggtac
     3601 aaaagtatct atatccacag taaaacgagt cctatatcga cataacctga aaggccactc
     3661 agcaaggaag aagccactgc tccaaaaccg acataagaaa gccagactac ggtttgcaac
     3721 tgcacatggg gacaaagatc gtactttttg gagaaatgtc ctctggtctg atgaaacaaa
     3781 aatagaactg tttggccata atgaccatcg ttatgtttgg aggaagaagg gggaggcttg
     3841 caagccgaag aacaccatcc caaccgtgaa gcacgggggt ggcagcatca tgttgtgggg
     3901 gtgctttgct gcaggaggga ctggtgcact tcacaaaata gatggcatca tggacgccgt
     3961 gcagtatgtg gatatattga agcaacatct caagacatca gtcaggaagt taaagcttgg
     4021 tcgcaaatgg gtcttccaac acgacaatga ccccaagcat acttccaaag ttgtggcaaa
     4081 atggcttaag gacaacaaag tcaaggtatt ggagtggcca tcacaaagcc ctgacctcaa
     4141 tcctatagaa aatttgtggg cagaactgaa aaagcgtgtg cgagcaagga ggcctacaaa
     4201 cctgactcag ttacaccagc tctgtcagga ggaatgggcc aaaattcacc caaattattg
     4261 tgggaagctt gtggaaggct acccgaaacg tttgacccaa gttaaacaat ttaaaggcaa
     4321 tgctaccaaa tactagctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     4381 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4441 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4501 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4561 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4621 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4741 tttcgctttc ttcccttcct ttctcgccac gttcgccacc ctaaagggag cccccgattt
     4801 agagcttgac ggggaaatgt gcggtatttc acaccgcata gatcggcaag tgcacaaaca
     4861 atacttaaat aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc
     4921 tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat
     4981 aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat
     5041 aaaatgtaag ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc
     5101 caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt
     5161 ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac
     5221 tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat
     5281 atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac
     5341 gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat
     5401 tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata taatacccag
     5461 caagtcagca tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac
     5521 tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt
     5581 tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc
     5641 ctctcctttt cttttttcga ccgaattaat tcttaatcgg caaaaaaaga aaagctccgg
     5701 atcaagattg tacgtaaggt gacaagctat ttttcaataa agaatatctt ccactactgc
     5761 catctggcgt cataactgca aagtacacat atattacgat gctgttctat taaatgcttc
     5821 ctatattata tatatagtaa tgtcgtgatc tatggtgcac tctcagtaca atctgctctg
     5881 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5941 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6001 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6061 tatttttata ggttaatgtc atgataataa tggtttctta gacggatcgc ttgcctgtaa
     6121 cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat
     6181 ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg
     6241 gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca
     6301 tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt
     6361 aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt
     6421 cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc
     6481 cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta
     6541 ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt atttttatag
     6601 cacgtgatga aaaggaccca gggaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     6661 cgggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6961 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7021 cagaagtatg caaagcacat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7081 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9301 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9361 gtatctgctc cctgcttgtg tgttggaggt c
//
