LOCUS       sequence-307167-       11720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin 
            resistant) off PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK FKBP12(F36V)-OGT (Neo)
FEATURES             Location/Qualifiers
     source          1..11720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4183..4693
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4612..4630
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4748..4772
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4803..4805,4806..5126)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             5133..5159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5133..5159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5163..5189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5205..5229
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8364..8388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8419..9007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8472..8492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8890..8901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9050..9549
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9085..9108)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9482..9501
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9563..10366
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9626..9645)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10236..10255
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             10457..10690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10762..10883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10799..10818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10853..10872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10923..11058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11078..11097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11079..11097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11107..11124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11107..11123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11116..11138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11265..11693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11352..11371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11562..11583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg gaattctgca
     4741 gatatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat
     4801 ccatgggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg
     4861 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct
     4921 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     4981 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     5041 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     5101 tcgatgtgga gcttctaaaa ctggaaggcg gctaccccta cgacgtgccc gactacgccg
     5161 gctatccgta tgatgtcccg gactatgcag gcatcgatag atcaacaagt ttgtacaaaa
     5221 aagcaggctt tgcgtcttcc gtgggcaacg tggccgacag cacagggtta gctgagttgg
     5281 cacatcgaga atatcaggca ggagattttg aggcagctga gagacactgc atgcagctct
     5341 ggagacaaga gccagacaat actggtgtgc ttttattact ttcatctata cacttccagt
     5401 gtcgaaggct ggacagatct gctcacttta gcactctggc aattaaacag aacccccttc
     5461 tggcagaagc ttattcgaat ttggggaatg tgtacaagga aagagggcag ttgcaggagg
     5521 caattgagca ttatcgacat gcattgcgtc tcaaacctga tttcatcgat ggttatatta
     5581 acctggcagc cgccttggta gcagcgggtg acatggaagg ggcagtacaa gcttacgtct
     5641 ctgctcttca gtacaatcct gatttgtact gtgttcgcag tgacctgggg aacctgctca
     5701 aagccctggg tcgcttggaa gaagccaagg catgttattt gaaagcaatt gagacgcaac
     5761 cgaactttgc agtagcttgg agtaatcttg gctgtgtttt caatgcacaa ggggaaattt
     5821 ggcttgcaat tcatcacttt gaaaaggctg tcacccttga cccaaacttt ctggatgctt
     5881 atatcaattt aggaaatgtc ttgaaagagg cacgcatttt tgacagagct gtggcagctt
     5941 atcttcgtgc cctaagtttg agtccaaatc acgcagtggt gcacggcaac ctggcttgtg
     6001 tatactatga gcaaggcctg atagatctgg caatagacac ctacaggcgg gctatcgaac
     6061 tacaaccaca tttccctgat gcttactgca acctagccaa tgctctcaaa gagaagggca
     6121 gtgttgctga agcagaagat tgttataata cagctctccg tctgtgtccc acccatgcag
     6181 actctctgaa taacctagcc aatatcaaac gagaacaggg aaacattgaa gaggcagttc
     6241 gcttgtatcg taaagcatta gaagtcttcc cagagtttgc tgctgcccat tcaaatttag
     6301 caagtgtact gcagcagcag ggaaaactgc aggaagctct gatgcattat aaggaggcta
     6361 ttcgaatcag tcctaccttt gctgatgcct actctaatat gggaaacact ctaaaggaga
     6421 tgcaggatgt tcagggagcc ttgcagtgtt atacgcgtgc catccaaatt aatcctgcat
     6481 ttgcagatgc acatagcaat ctggcttcca ttcataagga ttcagggaat attccagaag
     6541 ccatagcttc ttaccgcacg gctctgaaac ttaagcctga ttttcctgat gcttattgta
     6601 acttggctca ttgcctgcag attgtctgtg attggacaga ctatgatgag cgaatgaaga
     6661 agttggtcag tattgtggct gaccagttag agaagaatag gttgccttct gtgcatcctc
     6721 atcatagtat gctatatcct ctttctcatg gcttcaggaa ggctattgct gagaggcacg
     6781 gcaacctgtg cttagataag attaatgttc ttcataaacc accatatgaa catccaaaag
     6841 acttgaagct cagtgatggt cggctgcgtg taggatatgt gagttccgac tttgggaatc
     6901 atcctacttc tcaccttatg cagtctattc caggcatgca caatcctgat aaatttgagg
     6961 tgttctgtta tgccctgagc ccagacgatg gcacaaactt ccgagtgaag gtgatggcag
     7021 aagccaatca tttcattgat ctttctcaga ttccatgcaa tggaaaagca gctgatcgca
     7081 tccatcagga tggaattcat atccttgtaa atatgaatgg ctatactaag ggcgctcgaa
     7141 atgagctttt tgctctcagg ccagctccta ttcaggcaat gtggctggga taccctggga
     7201 cgagtggtgc gcttttcatg gattatatta tcactgatca ggaaacttcg ccagctgaag
     7261 ttgctgagca gtattccgag aaattggctt atatgcccca cacttttttt attggtgatc
     7321 atgctaatat gttccctcac ctgaagaaaa aagcagtcat cgattttaag tccaatgggc
     7381 acatttatga caatcggata gttctgaatg gcatcgacct caaagcattt cttgatagtc
     7441 taccagatgt gaaaattgtc aagatgaagt gtcctgatgg aggagacaat gcagatagca
     7501 gtaacacagc tcttaatatg cctgttattc ctatgaatac tattgcagaa gcagttattg
     7561 aaatgattaa ccgaggacag attcaaataa caattaatgg attcagtatt agcaatggac
     7621 tggcaactac tcagatcaac aataaggctg caactggaga ggaggttccc cgtaccatta
     7681 ttgtaaccac ccgttctcag tacgggttac cagaagatgc catcgtatac tgtaacttta
     7741 atcagttgta taaaattgac ccttctactt tgcagatgtg ggcaaacatt ctgaagcgtg
     7801 ttcccaatag tgtactctgg ctgttgcgtt ttccagcagt aggagaacct aatattcaac
     7861 agtatgcaca aaacatgggc ctgccccaga accgtatcat tttttcacct gttgctccta
     7921 aagaggaaca cgtcaggaga ggccagctgg ctgatgtctg cttggacact ccactctgta
     7981 atgggcacac cacagggatg gatgtcctct gggcagggac ccccatggtg actatgccag
     8041 gagagactct tgcttctcga gttgcagcat cccagctcac ttgcttaggt tgtcttgagc
     8101 ttattgctaa aaacagacaa gaatatgaag acatagctgt gaagctggga actgatctag
     8161 aatacctgaa gaaagttcgt ggcaaagtct ggaagcaaag aatatctagc cctctgttca
     8221 acaccaaaca atacacaatg gaactagagc ggctctatct acagatgtgg gagcattatg
     8281 cagctggcaa caaacctgac cacatgatta agcctgttga agtcactgag tcagcataac
     8341 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg atatccagca
     8401 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8461 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8521 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8581 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8641 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8701 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8761 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8821 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8881 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8941 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9001 tccccgcctg gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag
     9061 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     9121 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     9181 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     9241 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     9301 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     9361 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     9421 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     9481 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     9541 ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg
     9601 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9661 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9721 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     9781 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     9841 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     9901 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     9961 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10021 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10081 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10141 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10201 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10261 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10321 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag
    10381 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    10441 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
    10501 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10561 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10621 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10681 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    10741 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
    10801 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10861 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
    10921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10981 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    11041 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
    11101 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    11161 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    11221 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
    11281 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    11341 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    11401 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    11461 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    11521 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    11581 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    11641 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    11701 aatattaacg cttacaattt
//
