LOCUS       sequence-307172-        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of mgtC protein from S. 
            Typhimurium in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL13-MgtC bb118
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(26..69)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    72..93
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        94..128
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     primer_bind     complement(907..924)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tnnnnnaggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
       61 gatgatttct ggaattcgcg gccgcttcta gagttgacgg ctagctcagt cctaggtatt
      121 gtgctagcat taactttaag aaggagatat acatacccat ggaggaacgt atgttaatgt
      181 ttccttatat tttaaattta ctggccgcta tgctgttggg cgcgctgatt ggcgcggaaa
      241 gacaatggcg tcaacgtatg gcgggattac gcactaatgc gctggtggcg acaggcgccg
      301 ccgtttttat tctgagctcc atgacgacat cgccagacag ccccgggcga attgcggcgc
      361 agattgtctc tgggattggc tttctggggg cgggcgtcat catgcgcgaa gggatgaatg
      421 tgcgcgggct gaacactgcg gcaacgctat ggtgttcggc gggcatcggc gtgttatgcg
      481 gcttagggca gttcaaaaat gcgctggcgg cgacgatcat tattctttgc gccaatattt
      541 tattgcgtga agcggcgcag cgtataaatc aactcccaat atccgctgag ggagaaaaac
      601 gttatatcct gaaagtaacc tgtaataaag aagatgagag cgcggttcgt cagtggttac
      661 tgaatatcgt aaaagaggcc gcgatctgtt tacaagggtt aggttcggtc cccgctcang
      721 aacagggcta taaagaaatt cgtgccgagc tcgtgggtca tgctgattat cgtaaaacgc
      781 gagagctgat tatatccaga ataggtgata acgataatat caccgcaatt cactggagca
      841 ttgatagtca ataagtttaa acggtctcca gcttggctgt tttggcggat ganagaagat
      901 tttcagcctg atacagatta aatcanaacg cagaagcggt ctgataaaca gaatttgcct
      961 nnngcagtag cgcgnnnncc caccngaccc atgccgaact canaagtgaa acgccgtann
     1021 ncnatgnann gtggggnctc ccatgnnnna ntnnnnnctn cngnatcnaa tnaaacnaaa
     1081 nntcantcna anactggnnn nnnnnntcnn tnnnnnnact nncnnnnnng nnnnnnnngn
     1141 ngnnggnnnn nggnnnannt cncnnnnang nngnannacg
//
