LOCUS       sequence-307173-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of mgtC protein from S. 
            Typhimurium in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL13-MgtC bb118
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..27
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     224..241
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     gap             1060..1073
                     /estimated_length=14
ORIGIN
        1 accgagcgca gcgagtcagt gagcgaggaa gccaaggccc agtctttcga ctgagccttt
       61 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc
      121 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact
      181 gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag
      241 gctgaaaatc ttctctcatc cgccaaaaca gccaagctgg agaccgttta aacttattga
      301 ctatcaatgc tccagtgaat tgcggtgata ttatcgttat cacctattct ggatataatc
      361 agctctcgcg ttttacgata atcagcatga cccacgagct cggcacgaat ttctttatag
      421 ccctgttcct gagcggggac cgaacctaac ccttgtaaac agatcgcggc ctcttttacg
      481 atattcagta accactgacg aaccgcgctc tcatcttctt tattacaggt tactttcagg
      541 atataacgtt tttctccctc agcggatatt gggagttgat ttatacgctg cgccgcttca
      601 cgcaataaaa tattggcgca aagaataatg atcgtcgccg ccagcgcatt tttgaactgc
      661 cctaagccgc ataacacgcc gatgcccgcc gaacaccata gcgttgccgc agtgttcagc
      721 ccgcgcacat tcatcccttc gcgcatgatg acgcccgccc ccagaaagcc aatcccagag
      781 acaatctgcg ccgcaattcg cccggggctg tctggcgatg tcgtcatggn gctcagaata
      841 aaaacggcgg cgcctgtcgc caccagcgca ttagtgcgta atcccgccat acgttgacgc
      901 cattgtcttt ccgcgncaat cagcgcgccc aacagcatan cggccagtaa atttaaaata
      961 nnnnnaaacn ttnnntacgt tcctncatgg gtatgtatat ctnctnctta aagttaatgc
     1021 tagcananac ctnnnctgag ctagcngtca ntctanancn nnnnnnnnnn nnnatctnnc
     1081 gaagctannt ttttttnatc tgnantcnnc nnnnnntacn cnattttnnn nnnnngncan
     1141 gnnnaanaan nnnnntannn gncaggnnnn nttttnnngg naa
//
