LOCUS       sequence-307176-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of the ratiometric 
            mRuby-iATPSnFR1.0 ATP indicator in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL10-mRuby-iATPSnFR1.0 bb118
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    67..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        89..123
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     gap             1142..1161
                     /estimated_length=20
ORIGIN
        1 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
       61 tttctggaat tcgcggccgc ttctagagtt gacggctagc tcagtcctag gtattgtgct
      121 agcattaact ttaagaagga gatatacata cccatggtct ccaaggggga ggaacttatt
      181 aaagaaaata tgcgcatgaa agttgttatg gaggggtctg taaatggtca ccagtttaag
      241 tgcacagggg agggcgaggg acgtccttat gaaggggtcc aaaccatgcg cattaaggtg
      301 attgagggtg gcccgcttcc gttcgctttt gacatccttg ctacaagttt tatgtatgga
      361 agtcgtacct ttatcaaata tcccgcagat attccagatt ttttcaagca gagtttccct
      421 gagggcttca catgggagcg tgtcacccgt tacgaagacg gtggtgtagt caccgtcacc
      481 caagatacat ctctggaaga cggtgaactg gtgtacaacg taaaagtccg tggcgtaaat
      541 tttccatcta acggaccggt catgcagaag aaaaccaaag gttgggaacc taacacagag
      601 atgatgtatc ccgcagacgg gggcttacgt ggctatacgg acatcgcttt aaaggtggac
      661 ggagggggtc accttcactg taattttgtc acgacgtatc gttccaagaa aacagttgga
      721 aacatcaaga tgcctggggt acatgcagta gaccatcgcc ttgaacgcat cgaggagtcc
      781 gataatgaga cttacgtcgt gcagcgtgag gtagcagtcg ccaaatactc gaacctgggc
      841 gggggctcga tgaagacaat tcatgtctct gtagttacac ctgatggacc cgtgtacgag
      901 gacgacgtcn agatggtttc cgtcaaggca aatctggnga gctgggcatc ntaccaggan
      961 ntatcccact gannncccct nnaatctcgc ggcngcttaa aaaannagna gnnnnaatac
     1021 attgcagngn ncgggggnat ctgngntcgn ccnnannnnn nnnnctatgn nncngcancn
     1081 nannnnnnng ananatnnan nnactgnnnn nnncancnan gnnnnnnnan nnnncnnnan
     1141 cnnnnnnnnn nnnnnnnnnn ncgnnnnnan nnnnnggnnn nanccnnt
//
