LOCUS       sequence-307177-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of the ratiometric 
            mRuby-iATPSnFR1.0 ATP indicator in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL10-mRuby-iATPSnFR1.0 bb118
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..28
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     225..242
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 annnnnncgc agcgagtcag tgagcgagga agccaaggcc cagtctttcg actgagcctt
       61 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
      121 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
      181 tgccgccagg caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca
      241 ggctgaaaat cttctctcat ccgccaaaac agccaagctg gagaccgttt aaacctactt
      301 catctccgcg acagacagac ggttcatcgc acgtttaagg gctaattcag cacgtttgaa
      361 gtcgatatca tgcaacccga agttatactc taacttatgt cctaagatgt taccgtcttc
      421 tttgaaatcg attcccttta attcaatgcg gtttaccaga gtatcacctt caaacttaac
      481 ttcagcacgc gtcttatagg tgccgtcatc cttaaaacta atcgtacgtt cctgaacgta
      541 accctcaggc atggccgatt taaagaaatc gtgttgcttc atgtggtctg gatagcggct
      601 gaaacactga actccgtacg ttaaggtggt gactaaggtt ggccagggaa caggaagttt
      661 tccagttgtg cagataaatt taagagtcaa cttcccatta gtggcatccc cctctccttc
      721 accacgcact gagaacttat gcccgttaac atcaccatcc agctctacaa gaatcggaac
      781 tactccggta aacaattctt cacccttcga catagacccc ccggtacctc ccttgtaaag
      841 ctcgtccatc ccaagcgtaa tgccagcagc agtcacaaac tccagtaaca ccatatgatc
      901 acgcttctca ttcggatcct ttgacaggac ggactgcgta ctannnaatg gnnatctgga
      961 agaagcaccg ggccatctcg atcggggnnn ntttgctgan anggncannn nngcatgctn
     1021 cntcncannt tnngcnattt tgaaatngnt ttgatccgtt ttttttgntt ngnncanccg
     1081 taa
//
