LOCUS       sequence-307182-        4558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR
            with Level 0.5-compatible overhangs (Barley Construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71139
FEATURES             Location/Qualifiers
     source          1..4558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        403..2399
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(2548..2565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2658..3246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2738..2757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(4431..4535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccatgag ctccactctg tggtctcagg agctgcagtg cagcgtgacc
      421 cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca
      481 catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta
      541 aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa
      601 tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac
      661 tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac
      721 ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt
      781 ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa
      841 actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa
      901 agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt
      961 cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca
     1021 accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc
     1081 gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc
     1141 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct
     1201 cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt
     1261 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg
     1321 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct
     1381 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc
     1441 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
     1501 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
     1561 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
     1621 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt
     1681 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt
     1741 ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaatta
     1801 attctgtttc aaactacctg gtggatttat taattttgga tctgtatgtg tgtgccatac
     1861 atattcatag ttacgaattg aagatgatgg atggaaatat cgatctagga taggtataca
     1921 tgttgatgcg ggttttactg atgcatatac agagatgctt tttgttcgct tggttgtgat
     1981 gatgtggtgt ggttgggcgg tcgttcattc gttctagatc ggagtagaat actgtttcaa
     2041 actacctggt gtatttatta attttggaac tgtatgtgtg tgtcatacat cttcatagtt
     2101 acgagtttaa gatggatgga aatatcgatc taggataggt atacatgttg atgtgggttt
     2161 tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta
     2221 cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat
     2281 gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat
     2341 ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagt
     2401 acttgagacc acgaagtggg tacctcttaa ttaactggcc tcatgggcct tcacttcact
     2461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc atagctgttt
     2521 ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2581 ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
     3421 tatttaccca ccactttggt aatttcgcct ttcacgtaat gcacaaattc ttccagctga
     3481 tccgcacggc tggccagacg atcttcttcc tggcccagat acgcctgacg cgcttccaga
     3541 atcaccggct gatactgcgc cggcagacgt tccatggccc aatccgccgc cacatctttc
     3601 ggcgcaattt tgccggtcac cgcgctatac caaatacggc tcagggtcag caccacgttg
     3661 cgttcatcac ccgcccaatc cggcgggctg ttccacaggg tcagggtttc gttcagcgct
     3721 tcaaacaggt cctgttccgg caccggatca aacagttctt ccgctgccgg accaaccagc
     3781 gccacgctat gttcacgcgc tttggtcagc agaatggcca gatcgatatc aatggtcgcc
     3841 ggttcaaaaa tgccggccag aatatcgtta cgctgccatt cgccaaactg cagctcacgt
     3901 ttcgccggat aacgccacgg aataatgtca tcatgcacca cgatggtcac ttccaccgca
     3961 cgcagaattt cgctttcacc cgggctcgcg ctggtttcca gcagatcgtt aatcagcgca
     4021 cgacgggtgg tttcatccag acgcacggtc acggtcacca gcagatcaat atcgctatgc
     4081 ggtttcaggc cgccatccac cgcgctgcca tacagatgca cggccagcag ggtcggttcc
     4141 agatgacgtt caatcacgcc caccacttca gacagctggg tgctcacttc cgcaatcacc
     4201 gcttcacgca taatgttcag tttggttttc ggacggctgc cctgctgggt cacatcgttg
     4261 ctgctccaca gcatcaggca acggcccacc gcataacgcg cctgctgttt gctcgcacgc
     4321 ggcatgctct gcacgccaaa aaagcaatca tagcacgcca taaacaccgc caccgcacca
     4381 ttaccgccgc tacgttcggt cagggtacgg ctccaattac ggctacgcat actcttcctt
     4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
