LOCUS       sequence-30720-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Addgene QC next-generation sequencing identified retroviral plasmid 
            backbone elements..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Schweiger MR, You J, Howley PM
  TITLE     Bromodomain protein 4 mediates the papillomavirus E2 transcriptional
            activation function.
  JOURNAL   J Virol. 2006 May . 80(9):4276-85.
  PUBMED    16611886
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 May . 80(9):4276-85."
COMMENT     Sequence Label: p5189 pSUPER-shRNA-GFP
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(147..563)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(163..179)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(195..217)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tttggattgg gatcttataa gttctgtatg agaccacaga tccccgcaag ctgaccctga
       61 agttcttcaa gagagaactt cagggtcagc ttgctttttg gaaaagctta tcgataccgt
      121 cgacctcgag agatcgatct aattccggcg cctagagaag gagtgagggc tggataaagg
      181 gaggatcgag gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga
      241 agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc
      301 aaggcttccc aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa
      361 aagaccttga tcttaacctg ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat
      421 ccgacgttaa aggttggcca ttctgcagag cagaaggtaa cccaacgtct cttcttgaca
      481 tctaccgact ggttgtgagc gatccgctcg acatctttcc agtgacctaa ggtcaaactt
      541 aagggagtgg taacagtctg gccctaattt tcagacaaat acagaaacac agtcagacag
      601 agacaacaca gaacgatgct gcagcgctgc agcagacaag acgcgcggct tcggttccaa
      661 accgaaagca aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga
      721 accacatatc ctgacggggt cggattccac atcgactccc ttcctcaggt cgggccacaa
      781 aaacggcccc caaagtccct gggacgtctc ccagggttgc ggccgggtgt tcagaactcg
      841 tcagttccac cacgggtccg ccagatacag agctagttag ctaactagta cagacgcagg
      901 cgcaaacatt agatgccggc
//
