LOCUS       sequence-307209-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to 
            mScarlet fluorophores in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL09-GCaMP6f-mScarlet bb118
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcntccccc cggtagagtg gcgaccctca ctgcgctcgt attgttctac aactgtataa
       61 tcttcattat gcgaagtgat atctaactta cgatctacgt tatatgcgcc tggcatctga
      121 acaggtttct ttgccttgta cgtggtcttg aagtccgcta aataacgccc gccgtccttc
      181 aggcgaagtg ccattttgat atcccctttc aggacgccat cctctggata taagcgctcc
      241 gtactggctt cccagcccat agttttcttt tgcataactg gaccgtcggg aggaaaattc
      301 gtcccacgca actttacctt gtagatcaat gttccatcct ccaaactggt gtcttgcgtc
      361 acggtcacag ctccgccgtc ctcgaaattc atcacacgtt cccacttaaa tccctcggga
      421 aaagattgtt tgtaataatc cgggatatcg gccgggtgct tcgtgaaagc gcggctcccg
      481 tacataaatt ggggagaaag gatgtcccaa gaaaagggca aaggtccccc cttggtgacc
      541 ttcaacttgg cggtctgtgt cccttcgtac ggacgcccct caccctcacc ctcaatctca
      601 aattcgtgtc cgttcattga accctccatg tgtactttaa aacgcatgaa ctctttaatg
      661 gatccaccgc taccaccgct accaccggat ccacccttcg ctgtcatcat ttgtacaaac
      721 tcttcgtagt ttacctgacc atccccatcg atgtctgctt ccctgatcat ttcatcaacc
      781 tcttcatctg ttaacttctc tccaaggttt gtcatcacgt ggcgaagctc tgctgcactg
      841 atgtagccat tgccatcctt atcaaacaca ccgaacgctt ctctaatttc ttcttccgtg
      901 tccctgtatt tcatttttct tgccatcatt gtcagnaact cagggaagtc gatngngccg
      961 tcancgncgg catctacttc attgatcatg tcctgcagcn nctgctncnn nggg
//
