LOCUS       sequence-307212-        8674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8674)
  TITLE     GFP encoding lentivirus pLX302 EGFP-V5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLX302 EGFP-V5
FEATURES             Location/Qualifiers
     source          1..8674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2232..2831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2232..2251)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2728..2748
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2873..2990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3107..3410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3411..3614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3565..3585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3637..3661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3682..3684,3685..3687,3688..4398)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3727..3748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3988..4007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4335..4356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4418..4442)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4444..4485
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4527..5115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4580..4600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4998..5009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5187..5420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5498..5619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5535..5554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5589..5608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5659..5794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5814..5833)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5815..5833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5843..5860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5843..5859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5852..5874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6001..6456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6088..6107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6298..6319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6482..6586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6587..6655,6656..7447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6805..6824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7618..8206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8107..8126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8360..8377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8494..8515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8530..8535,8536..8553,8554..8560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8568..8584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8573..8595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8592..8608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8592..8608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8627..8647
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8629..8647
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2281 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2341 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2401 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
     2461 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2521 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2581 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     2641 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     2701 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     2761 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     2821 ccggtgcctg agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     2881 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2941 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     3001 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt tattaatagt
     3061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3601 atataagcag agctctctgg ctaactgtcg ggatcaacaa gtttgtacaa aaaagcaggc
     3661 tccgcggccg cccccttcac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag
     3781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4081 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4141 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     4201 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4261 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4321 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4381 atggacgagc tgtacaagtc caagggtggg cgcgccgacc cagctttctt gtacaaagtg
     4441 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
     4501 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4561 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4621 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4681 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4741 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4801 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4861 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4921 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4981 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5041 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5101 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     5161 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5221 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5281 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5341 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5401 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     5461 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5521 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5641 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5701 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5761 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5821 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5881 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5941 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6001 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6061 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8401 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8461 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8521 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8581 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8641 ctaaagggaa caaaagctgg agctgcaagc ttaa
//
