LOCUS       sequence-307214-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to 
            pHuji fluorophores in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL12-GCaMP6f-pHuji bb118
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcnnnnnacc accggtagag tgcctgccct cagccctctc gtactgttca acgatagtat
       61 aatcttcatt atgagagacg atgtccaatt ttatatcgac aatataggca ccgggtaact
      121 gtactggttt cttagcttta taagtagtct ttacttcagc tgcgtagtgg ccgccatctt
      181 tcagtttcag acgatattta atttcggact ttaaggcgcc atcctctggg tacattcttt
      241 cttctgaagc ttcccagccc atcgtcttct tttgcatcac tggaccatct gagggaaagt
      301 tagttcccct taacttaact ttgtaaatga acacaccatc ctgtaaagaa gaatcctgat
      361 taacatggat tatgccacca tcctcgaaat tcatgactct ctcccatcta aaaccttcag
      421 ggaatgacaa tttaaaataa tcaggaatgt ctgctggatg tttaatataa acttttgaac
      481 catacataaa ttgaggagaa aggatatccc acgcaaacgg taaaggtcca cctttagtga
      541 ctttcagttt ggcagtctgg aacgcctcgt atggtctacc ttcgccttca ccctcgattt
      601 cgaactcatg gccgttcact gaaccttcca tgtggacctt aaacctcatg aactctttga
      661 taatagccat gttgttctct tcacctttag aaacggatcc accgctacca ccgctaccac
      721 cggatccacc cttcgctgtc atcatttgta caaactcttc gtagtttacc tgaccatccc
      781 catcgatgtc tgcttccctg atcatttcat caacctcttc atctgttaac ttctctccaa
      841 ggtttgtcat cacgtggcga agctctgctg cactgatgta gccattgcca tccttatcaa
      901 acacaccgaa cgcttctcta atttcttctt ccgtgtccct gtatttcatt tttcttgcca
      961 tcattgtcag gaactcnggn ngtcgattgt gccgtcacgt cggcatctac ttcattgatc
     1021 atgtcctgca gctctgcttc tnnnggggnn ctgccccana nacngcatcn ncngtccc
//
