LOCUS       sequence-307239-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Peng W, Wu Z, Song K, Zhang S, Li Y, Xu M
  TITLE     Regulation of sleep homeostasis mediator adenosine by basal 
            forebrain glutamatergic neurons.
  JOURNAL   Science. 2020 Sep 4;369(6508). pii: 369/6508/eabb0556. doi: 
            10.1126/science.abb0556.
  PUBMED    32883833
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-09-4"; volume: "369(6508). pii: 369/6508/eabb0556. doi";
            pages: " 10.1126/science.abb0556"
COMMENT     Sequence Label: pAAV-EFS-DIO-GRAB_Ado1.0
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   124..253
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(395..414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct
       61 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccaagctta cgcgtgcggc
      121 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
      181 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
      241 agctgcctgc aggacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      361 tcangtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      661 gnagcagcca ctggnaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      721 ttgaagtgng gnctaactac ggctacacta gaagaacagt atttggnnat ctgcnctnnt
      781 gctgaagcca gttaccttcn gnaaaagagt tggnagctct gatc
//
