LOCUS       sequence-307242-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high 
            affinity G-protein coupled receptor that mobilizes Ca2+ from 
            intracellular stores upon binding extracellular ATP.48,49 By using 
            the Gibson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Mazzarda F, D'Elia A, Massari R, De Ninno A, Bertani FR, Businaro L,
            Ziraldo G, Zorzi V, Nardin C, Peres C, Chiani F, Tettey-Matey A, 
            Raspa M, Scavizzi F, Soluri A, Salvatore AM, Yang J, Mammano F
  TITLE     Organ-on-chip model shows that ATP release through connexin 
            hemichannels drives spontaneous Ca(2+) signaling in non-sensory 
            cells of the greater epithelial ridge in the developing cochlea.
  JOURNAL   Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc00427h.
  PUBMED    32700707
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lab Chip. 
            2020 Jul 23. doi: 10.1039/d0lc00427h."
COMMENT     Sequence Label: pHOT-P2Y2R
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    92..681
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(145..165)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(563..574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aggcacaatg gcgggacgaa gttcacgccg gctggtagcg agaacactaa ggacattcgg
       61 ctgtagaacg ttccggagtc gtccactcga caatcaacct ctggattaca aaatttgtga
      121 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      181 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      241 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      301 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      361 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      421 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      481 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
      541 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
      601 gctgccgggc tctgcggcct cttccgcctc ttcgccttcg ccctcagacg agtcggatct
      661 ccctttgggc cgcctccccg cctggaatta attcgagctc ggtaccttta agaccaatga
      721 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaaaggg
      781 ctacgtaact cccaacgaag acaagatctg cttttttgct tgtactgggt ctctctggtt
      841 agaccagatc tgagcctggg gagctctctg gctaactagg ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta atggtgtgcc cgtctgttgg ggggactctg
      961 gtaactagag atccctccag acccttttag tccgggtggg gaaaaattct ccaagcagaa
     1021 gaagttcagg gcaccttatt attc
//
