LOCUS       sequence-307243-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high 
            affinity G-protein coupled receptor that mobilizes Ca2+ from 
            intracellular stores upon binding extracellular ATP.48,49 By using 
            the Gibson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Mazzarda F, D'Elia A, Massari R, De Ninno A, Bertani FR, Businaro L,
            Ziraldo G, Zorzi V, Nardin C, Peres C, Chiani F, Tettey-Matey A, 
            Raspa M, Scavizzi F, Soluri A, Salvatore AM, Yang J, Mammano F
  TITLE     Organ-on-chip model shows that ATP release through connexin 
            hemichannels drives spontaneous Ca(2+) signaling in non-sensory 
            cells of the greater epithelial ridge in the developing cochlea.
  JOURNAL   Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc00427h.
  PUBMED    32700707
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lab Chip. 
            2020 Jul 23. doi: 10.1039/d0lc00427h."
COMMENT     Sequence Label: pHOT-P2Y2R
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..102
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 accggggata taacaggagg agaaatgaaa gccatacggg aagcaatagc atgatacaaa
       61 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
      121 aatctttcac aaattttgta atccagaggt tgattgtcga gtcgacgact ccggaacgtt
      181 ctacagccga atgtccttag tgttctcgct accagccggc gtggactctg tccgcctaga
      241 gtcctcactg ctgcccaaca catctcctat cctctgcatg tcagttctgt cggatctgcg
      301 caggcccagc ctgcggcgag ccggggtggc agggctgggg ccagtgggtg gcttggcatc
      361 tcgggcaaag cgtacgagcc tctgcccagc caggaagtag agcacggggt caaggcaact
      421 gttagcactg gccagcggcc gggtaacctt gtaggccatg ttgatggcgt tgagggtgtg
      481 gcagctgagg tccagcgagc ggaaggagta gtagagggtg cgggtgacgt ggaatggcag
      541 gaagcagagg gcgaagacag ccagcaccac ggcgatggtg cgcacggact tgcgcttggc
      601 cctaggcagg ccgcccgagg tcccgtaggc tggctttagc agtcgccgag ccatgagcac
      661 gtaacagaca aggatgacgg caaagggcac cgcgaagagc aggcccagca tgactgagct
      721 gtaggccacg aagcggctga agagctcggg tgccgaggtg tcgtggcagg ttacgcggcc
      781 cccgcgcgcg ctggtggtga caaagtagag cacgggggcc tggcaggcca gcaccaacac
      841 ccacacggcc ccggccaccc ggcgagcgta gcgggcccgg ccccagcgca gggagcgcag
      901 aggtcgtaag acgcccagac accggtgcac gctgatgcag gtgaggaaaa ggatgctgca
      961 gtaaaggttg gtgtaaaaga ggaagcgcac cagcttgcaa agcaccgtgc tgaagggcca
     1021 atggtcgccg cgggcgtaat attagaccag caccggcagg ggaggccgca tacagtgcat
     1081 caaaaccagc ccaggtggaa cattatgtgg gtggacgctt tccaggtctt gaggcggccc
     1141 aaaaaaaatg taaacgccac ggggttcaaa acaagcccaa gccgccacac accccgttag
     1201 gaaaaagggg caaacccgta ctttaaatcc tctgttagag ggccctttga ccccccttac
//
