LOCUS       sequence-307262-       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134182
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    78..149
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(154..200)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    218..266
                     /note="synthetic polyadenylation signal"
     enhancer        347..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     805..825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4748..4768
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4769..4795
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4864..4985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4875..4894)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4929..4948
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5100..5146
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5176..5312
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5357..5428
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5654..6242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5734..5753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6742..7951
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7788..7807
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7955..8983
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    9010..9058
                     /note="synthetic polyadenylation signal"
     CDS             join(9324..9392,9393..10184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9542..9561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10197..10333
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca
       61 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
      121 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      181 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      241 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      661 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      901 ctcgacacac ccgccagcgg ccgccaccat gttcgtcttt cttgtgttac tccccctggt
      961 ttcgtcccag tgcgtgaact tgactacgcg aacccaactg ccgccagcat atacaaatag
     1021 tttcacccgt ggagtatact accctgataa agtgtttaga tcaagcgtac tacactctac
     1081 tcaggacctg tttctcccct tcttctccaa tgtcacatgg tttcatgcga tccacgttag
     1141 cggcaccaac gggacaaagc gctttgacaa ccctgtgctg ccattcaatg atggtgtgta
     1201 ttttgcttct accgagaaga gcaacataat tcggggctgg atcttcggaa ctaccctcga
     1261 cagtaaaact cagtcattgc tgattgtgaa taacgccaca aatgtcgtta tcaaggtctg
     1321 tgaatttcaa ttctgcaacg atccattcct tggggtgtat taccataaaa ataataagtc
     1381 ctggatggag agcgaattta gggtatactc gtctgccaac aattgtactt tcgagtatgt
     1441 gtctcagccc ttcttaatgg atctggaagg taaacagggc aactttaaga acctccgcga
     1501 gtttgtattc aaaaatatag acggatactt taagatttat tccaagcaca caccaatcaa
     1561 tttggtgcgg gatctcccgc aagggttcag cgctctggaa cctctagtgg acctccccat
     1621 cggcattaac atcacaagat tccagacgct gttagccctg catagatcat accttactcc
     1681 cggtgacagt agttctgggt ggaccgcagg cgcggctgca tattacgtgg gatatttgca
     1741 gcctcggacg tttctgctga aatacaacga gaacggcacc attacagatg ccgtggattg
     1801 cgcccttgac cccctaagcg aaactaagtg tacgcttaaa agcttcaccg tcgagaaggg
     1861 aatttaccag acttccaact ttagggtcca gccgacagaa tcaatagtta ggttcccaaa
     1921 tatcaccaat ttgtgccctt ttggggaagt cttcaatgct acacgatttg cttcggttta
     1981 tgcctggaac aggaagcgta tctcaaattg cgtcgcggac tacagcgtgc tctataatag
     2041 tgcaagtttc tcgaccttta aatgttatgg cgtgtccccg accaaactaa atgacctctg
     2101 ttttacaaac gtttacgccg attcttttgt gattcgcggt gatgaggtac gccaaatcgc
     2161 accgggccag actggcaaga ttgccgatta caactacaag ttgcctgacg acttcaccgg
     2221 atgtgttata gcctggaata gcaataatct ggattctaag gtcggaggta actataacta
     2281 cctgtataga ctgttccgta agtcaaacct gaaacccttt gagcgggaca tctccacaga
     2341 gatttaccaa gcagggtcta ccccctgcaa tggggttgaa ggattcaatt gctactttcc
     2401 attacagagc tacgggttcc agcctacgaa cggggttggt taccagcctt atagggtagt
     2461 ggtgctgtcg ttcgaactgt tgcacgctcc agctacagtg tgcgggccta aaaaatcaac
     2521 taacctggtt aaaaataagt gtgttaattt taacttcaat ggcttaaccg gaactggagt
     2581 gctcacggag tcaaacaaga aatttctacc gtttcagcaa ttcgggaggg atatcgcaga
     2641 cactacggac gcggtcagag atccacagac ccttgagatc cttgacataa caccctgctc
     2701 tttcggaggt gtctccgtaa ttacaccagg cactaatacc tctaatcagg tggccgtgct
     2761 ttatcaggat gttaactgta cagaggtccc ggtggctatc catgccgacc aattgacacc
     2821 tacgtggcgc gtctattcta ccggctccaa cgtcttccag acccgggctg gatgcttgat
     2881 aggcgcagaa cacgtaaaca actcttacga atgtgatatt cccattggag ccgggatctg
     2941 cgcaagctac cagacccaaa caaacagtcc cagacgggct cgtagcgtgg cctctcagag
     3001 cataatagcc tataccatgt cccttggcgc ggagaatagt gtggcatact caaataatag
     3061 catcgctatc cccactaact ttactatttc cgtgacgact gagattctgc cagtgtctat
     3121 gactaagaca tccgtggatt gcacaatgta tatttgtggg gattccacag agtgtagcaa
     3181 cttgttgctg caatatggat cattctgtac gcagctgaat cgcgccctca ccggcatcgc
     3241 tgtggaacag gacaagaaca ctcaagaggt ctttgctcag gtgaagcaaa tctacaagac
     3301 cccacctata aaagacttcg ggggttttaa cttctcgcag attctccccg accccagcaa
     3361 gccttcaaaa aggagcttta ttgaggacct gttgttcaac aaggttactt tagccgatgc
     3421 cggttttatc aaacagtacg gcgactgtct tggcgatata gcagcccggg atctgatttg
     3481 cgcacaaaaa ttcaatggcc ttaccgtact gccacccctt ctcaccgacg aaatgatcgc
     3541 gcagtacacc tccgcactgc tggctgggac catcacttcg ggatggacct ttggggctgg
     3601 tgctgctttg caaattccct tcgccatgca gatggcctat cgattcaatg gcataggggt
     3661 aacacagaac gtattgtatg aaaatcaaaa gctaatcgct aaccagttta attcagccat
     3721 tggtaagatc caggattcac tctccagtac agcctctgcc ctgggaaagc tccaggacgt
     3781 ggtcaaccag aacgcgcagg cattgaatac actcgtcaaa cagctgagta gcaatttcgg
     3841 cgcaatcagt agtgtcttaa acgatatact gagtaggctt gataaagtag aggccgaggt
     3901 ccagattgac agactaatca cggggcgtct tcagagttta cagacctatg tgacccaaca
     3961 gctgatccga gcagcggaaa ttcgagcaag cgcgaacctg gccgctacta aaatgagtga
     4021 gtgcgtgctg ggccaaagta aacgagtcga cttttgcggc aaagggtatc atctcatgtc
     4081 tttccctcag tccgcccctc acggtgttgt tttcctccac gtgacatacg ttcccgcgca
     4141 ggaaaagaat tttactacgg cacccgctat ttgccatgac ggaaaggctc attttccacg
     4201 ggagggagtg ttcgtttcaa acgggacaca ctggtttgta actcagcgga acttttatga
     4261 gcctcaaatc atcacgacag acaatacctt cgtgagtggc aactgcgatg tagtcatagg
     4321 cattgtgaat aacactgttt atgatccact tcagccggaa ctcgacagct tcaaagagga
     4381 attggataag tactttaaaa atcataccag tccagacgta gatctcggcg atatatcggg
     4441 cattaacgcc agtgtggtca atatacaaaa ggagattgac agactgaatg aagtagctaa
     4501 gaacctcaac gaatctctga ttgacctgca agagctaggg aagtacgagc agtatatcaa
     4561 atggccatgg tacatatggc tgggttttat cgcgggtctt attgccatag tcatggtgac
     4621 gattatgttg tgctgtatga cctcctgctg ttcttgtttg aaaggctgct gctcctgtgg
     4681 tagctgttgc aaattcgatg aagatgactc tgaacccgtg ttgaaaggcg ttaagctaca
     4741 ctacacagaa aacctttact ttcagtcgta cccctatgac gtaccagatt atgcgtaagg
     4801 atccgcaggc ctctgctagc ttgactgact gagatacagc gtaccttcag ctcacagaca
     4861 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4921 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4981 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5041 ttttttaaag caagtaaaac ctctacaaat gtggtattgg cccatctcta tcggtatcgt
     5101 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgtgcc cctcgggccg
     5161 gattgctatc taccggcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta
     5221 tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat
     5281 acgtgtcctc cttaccagaa atttatcctt aaggtcgtca gctatcctgc aggcgatctc
     5341 tcgatttcga tcaagacatt cctttaatgg tcttttctgg acaccactag gggtcagaag
     5401 tagttcatca aactttcttc cctccctaat ctcattggtt accttgggct atcgaaactt
     5461 aattaaccag tcaagtcagc tacttggcga gatcgacttg tctgggtttc gactacgctc
     5521 agaattgcgt cagtcaagtt cgatctggtc cttgctattg cacccgttct ccgattacga
     5581 gtttcattta aatcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcat
     6481 ttaaatttcc gaactctcca aggccctcgt cggaaaatct tcaaaccttt cgtccgatcc
     6541 atcttgcagg ctacctctcg aacgaactat cgcaagtctc ttggccggcc ttgcgccttg
     6601 gctattgctt ggcagcgcct atcgccaggt attactccaa tcccgaatat ccgagatcgg
     6661 gatcacccga gagaagttca acctacatcc tcaatcccga tctatccgag atccgaggaa
     6721 tatcgaaatc ggggcgcgcc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     6781 ccctcgtcac ggcgagcgct gccacgtcag acgaagggcg caggagcgtc ctgatccttc
     6841 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     6901 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     6961 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     7021 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     7081 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     7141 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     7201 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     7261 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     7321 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     7381 acccaaggtc ttgagccctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     7441 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     7501 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     7561 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     7621 ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     7681 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     7741 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     7801 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
     7861 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag acttgtaaat tgtccgctaa
     7921 attctggccg tttttggctt ttttgttaga caacatgggt aaaaagcctg aactcaccgc
     7981 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     8041 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     8101 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     8161 atccgccgcg ctcccgattc cggaagtgct tgacattggg gagttcagcg agagcctgac
     8221 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     8281 gcccgctgtt ctgcagccgg tcgcggaggc aatggatgcc atcgctgccg ccgatcttag
     8341 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     8401 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     8461 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     8521 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     8581 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     8641 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     8701 cttcgagcgg aggcatccgg agcttgcagg atcgccccgg ctccgggcgt atatgctccg
     8761 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     8821 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     8881 aatcgcccgc agaagcgccg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     8941 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taagctagta tgtaagccta
     9001 gtcttagata ataaaatcgc tatccatcga agatggatgt gtgttggttt tttgtgtgtg
     9061 taacgctagg cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc
     9121 atatcgttgc ttggcagtca gccagtcgga atccagcttg ggacccagga agtccaatcg
     9181 tcagatattg tactcaagcc tggtcacggc agcgtaccga tctgtttaaa cctagatatt
     9241 gatagtctga tcggtcaacg tataatcgag tcctagcttt tgcaaacatc tatcaagaga
     9301 caggatcagc aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc cttattccct
     9361 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9421 atgctgaaga tcagttgggt gcgcgagtgg gttacatcga actggatctc aacagcggta
     9481 agatccttga gagttttcgc cccgaagaac gctttccaat gatgagcact tttaaagttc
     9541 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9601 tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg
     9661 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9721 ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca
     9781 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9841 acgacgagcg tgacaccacg atgcctgtag caatggcaac aaccttgcgt aaactattaa
     9901 ctggcgaact acttactcta gcttcccggc aacagttgat agactggatg gaggcggata
     9961 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10021 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10081 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10141 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat
    10201 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
    10261 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
    10321 aatttatcct taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc
    10381 gtttcgagct tacgcgaaca gccgtgg
//
