LOCUS       sequence-307301-        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133919
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             209..250
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    303..424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(314..333)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     368..387
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      539..585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      615..751
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    796..867
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1093..1681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1173..1192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2427..2495,2496..3287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2645..2664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3300..3436
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3588..3659
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3664..3710)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3728..3776
                     /note="synthetic polyadenylation signal"
     enhancer        3857..4160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4162..4364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4315..4335
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgttt catctcgttg actttcaggt tactatagca gagatattac taattattat
       61 gaggactttt aaagtttcca tttggaatct tgattacatc ataaacctca taattaaaaa
      121 tttatctaag tcactaactg agaataaata ttctcaatta gatgaagagc aaccaatgga
      181 gattgatgaa aacctttact ttcagtcggg gaaacctata cccaatccac ttctcggctt
      241 agattctacc taagctagct tgactgactg agatacagcg taccttcagc tcacagacat
      301 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      361 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      421 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
      481 tttttaaagc aagtaaaacc tctacaaatg tggtattggc ccatctctat cggtatcgta
      541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgtgccc ctcgggccgg
      601 attgctatct accggcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat
      661 actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata
      721 cgtgtcctcc ttaccagaaa tttatcctta aggtcgtcag ctatcctgca ggcgatctct
      781 cgatttcgat caagacattc ctttaatggt cttttctgga caccactagg ggtcagaagt
      841 agttcatcaa actttcttcc ctccctaatc tcattggtta ccttgggcta tcgaaactta
      901 attaaccagt caagtcagct acttggcgag atcgacttgt ctgggtttcg actacgctca
      961 gaattgcgtc agtcaagttc gatctggtcc ttgctattgc acccgttctc cgattacgag
     1021 tttcatttaa atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcatt
     1921 taaatttccg aactctccaa ggccctcgtc ggaaaatctt caaacctttc gtccgatcca
     1981 tcttgcaggc tacctctcga acgaactatc gcaagtctct tggccggcct tgcgccttgg
     2041 ctattgcttg gcagcgccta tcgccaggta ttactccaat cccgaatatc cgagatcggg
     2101 atcacccgag agaagttcaa cctacatcct caatcccgat ctatccgaga tccgaggaat
     2161 atcgaaatcg gggcgcgcct ggtgtaccga gaacgatcct ctcagtgcga gtctcgacga
     2221 tccatatcgt tgcttggcag tcagccagtc ggaatccagc ttgggaccca ggaagtccaa
     2281 tcgtcagata ttgtactcaa gcctggtcac ggcagcgtac cgatctgttt aaacctagat
     2341 attgatagtc tgatcggtca acgtataatc gagtcctagc ttttgcaaac atctatcaag
     2401 agacaggatc agcaggaggc tttcgcatga gtattcaaca tttccgtgtc gcccttattc
     2461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2521 aagatgctga agatcagttg ggtgcgcgag tgggttacat cgaactggat ctcaacagcg
     2581 gtaagatcct tgagagtttt cgccccgaag aacgctttcc aatgatgagc acttttaaag
     2641 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2701 gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta
     2761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2821 cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca
     2881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2941 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaaccttg cgtaaactat
     3001 taactggcga actacttact ctagcttccc ggcaacagtt gatagactgg atggaggcgg
     3061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaccg attctaggtg
     3301 cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc
     3361 agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc
     3421 agaaatttat ccttaagatc ccgaatcgtt taaactcgac tctggctcta tcgaatctcc
     3481 gtcgtttcga gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc
     3541 gtcagctatc gtcagcttac ctttttggca gcgatcgcgg ctcccgacat cttggaccat
     3601 tagctccaca ggtatcttct tccctctagt ggtcataaca gcagcttcag ctacctctca
     3661 attcaaaaaa cccctcaaga cccgtttaga ggccccaagg ggttatgcta tcaatcgttg
     3721 cgttacacac acaaaaaacc aacacacatc catcttcgat ggatagcgat tttattatct
     3781 aactgctgat cgagtgtagc cagatctagt aatcaattac ggggtcatta gttcatagcc
     3841 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3901 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3961 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4021 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4081 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4141 tagtcatcgc tattaccatg ctgatgcggt tttggcagta catcaatggg cgtggatagc
     4201 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4261 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4321 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     4381 agatcagatc tttgtcgatc ctaccatcca ctcgacacac ccgccagcgg ccgc
//
