LOCUS       sequence-30733-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M
  TITLE     A streptavidin variant with slower biotin dissociation and increased
            mechanostability.
  JOURNAL   Nat Methods. 2010 May;7(5):391-3. doi: 10.1038/nmeth.1450. Epub 2010
            Apr 11.
  PUBMED    20383133
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1450"; journalName: "Nat Methods"; date: "2010-05"; 
            volume: "7"; issue: "5"; pages: "391-3"
COMMENT     Sequence Label: pET21a-Traptavidin
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(70..87)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             479..501
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    516..540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(540..559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(541..559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(643..662)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgggcttgtt gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttttattaa tggtggtgat ggtgatggga agcagcggac ggtttaactt tggtgaaggt
      121 gtcgtgacca accagggtgg atttccacgc gttggcttcg gtggtgccgg aggtcaacaa
      181 ccactgggtg ttgatacgag cttcagcacc accaacgtac tggccagacc aggtggtagc
      241 ggagtgagcg ttacggtagt tgtttttcca agcaacggtc caacccagag cggttccgga
      301 accgtcggta gccggagcgg agtcgtaacg accggtcaga acgtaatcgc cttcagcgtt
      361 accaacagcg gattcgtagg taccggtcag agcaccgtca gcaccagcgg taacgatgaa
      421 ggtggatccc agctggttgt accaggtgcc ggtgatacca gcttcagcca tatgtatatc
      481 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
      541 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca
      601 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
      661 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
      721 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
      781 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
      841 cgcata
//
