LOCUS       sequence-307331-        8300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature 
            peptide 3 to orf1a stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8300)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4092694
FEATURES             Location/Qualifiers
     source          1..8300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5183..5230
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5273..5304
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        5305..5407
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(5408..5476,5477..6268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5626..5645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             6269..6460
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      6484..6511
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(6523..7110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(7132..7161)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 catattcagt ggatggttat gttcacacct ttagtacctt tctggataac aattgcttat
       61 atcatttgta tttccacaaa gcatttctat tggttcttta gtaattacct aaagagacgt
      121 gtagtcttta atggtgtttc ctttagtact tttgaagaag ctgcgctgtg cacctttttg
      181 ttaaataaag aaatgtatct aaagttgcgt agtgatgtgc tattacctct tacgcaatat
      241 aatagatact tagctcttta taataagtac aagtatttta gtggagcaat ggatacaact
      301 agctacagag aagctgcttg ttgtcatctc gcaaaggctc tcaatgactt cagtaactca
      361 ggttctgatg ttctttacca accaccacaa acctctatca cctcagctgt tttgcagagt
      421 ggttttagaa aaatggcatt cccatctggt aaagttgagg gttgtatggt acaagtaact
      481 tgtggtacaa ctacacttaa cggtctttgg cttgatgacg tagtttactg tccaagacat
      541 gtgatctgca cctctgaaga catgcttaac cctaattatg aagatttact cattcgtaag
      601 tctaatcata atttcttggt acaggctggt aatgttcaac tcagggttat tggacattct
      661 atgcaaaatt gtgtacttaa gcttaaggtt gatacagcca atcctaagac acctaagtat
      721 aagtttgttc gcattcaacc aggacagact ttttcagtgt tagcttgtta caatggttca
      781 ccatctggtg tttaccaatg tgctatgagg cccaatttca ctattaaggg ttcattcctt
      841 aatggttcat gtggtagtgt tggttttaac atagattatg actgtgtctc tttttgttac
      901 atgcaccata tggaattacc aactggagtt catgctggca cagacttaga aggtaacttt
      961 tatggacctt ttgttgacag gcaaacagca caagcagctg gtacggacac aactattaca
     1021 gttaatgttt tagcttggtt gtacgctgct gttataaatg gagacaggtg gtttctcaat
     1081 cgatttacca caactcttaa tgactttaac cttgtggcta tgaagtacaa ttatgaacct
     1141 ctaacacaag accatgttga catactagga cctctttctg ctcaaactgg aattgccgtt
     1201 ttagatatgt gtgcttcatt aaaagaatta ctgcaaaatg gtatgaatgg acgtaccata
     1261 ttgggtagtg ctttattaga agatgaattt acaccttttg atgttgttag acaatgctca
     1321 ggtgttactt tccaaagtgc agtgaaaaga acaatcaagg gtacacacca ctggttgtta
     1381 ctcacaattt tgacttcact tttagtttta gtccagagta ctcaatggtc tttgttcttt
     1441 tttttgtatg aaaatgcctt tttacctttt gctatgggta ttattgctat gtctgctttt
     1501 gcaatgatgt ttgtcaaaca taagcatgca tttctctgtt tgtttttgtt accttctctt
     1561 gccactgtag cttattttaa tatggtctat atgcctgcta gttgggtgat gcgtattatg
     1621 acatggttgg atatggttga tactagtttg tctggtttta agctaaaaga ctgtgttatg
     1681 tatgcatcag ctgtagtgtt actaatcctt atgacagcaa gaactgtgta tgatgatggt
     1741 gctaggagag tgtggacact tatgaatgtc ttgacactcg tttataaagt ttattatggt
     1801 aatgctttag atcaagccat ttccatgtgg gctcttataa tctctgttac ttctaactac
     1861 tcaggtgtag ttacaactgt catgtttttg gccagaggta ttgtttttat gtgtgttgag
     1921 tattgcccta ttttcttcat aactggtaat acacttcagt gtataatgct agtttattgt
     1981 ttcttaggct atttttgtac ttgttacttt ggcctctttt gtttactcaa ccgctacttt
     2041 agactgactc ttggtgttta tgattactta gtttctacac aggagtttag atatatgaat
     2101 tcacagggac tactcccacc caagaatagc atagatgcct tcaaactcaa cattaaattg
     2161 ttgggtgttg gtggcaaacc ttgtatcaaa gtagccactg tacagtctaa aatgtcagat
     2221 gtaaagtgca catcagtagt cttactctca gttttgcaac aactcagagt agaatcatca
     2281 tctaaattgt gggctcaatg tgtccagtta cacaatgaca ttctcttagc taaagatact
     2341 actgaagcct ttgaaaaaat ggtttcacta ctttctgttt tgctttccat gcagggtgct
     2401 gtagacataa acaagctttg tgaagaaatg ctggacaaca gggcaacctt acaagctata
     2461 gcctcagagt ttagttccct tccatcatat gcagcttttg ctactgctca agaagcttat
     2521 gagcaggctg ttgctaatgg tgattctgaa gttgttctta aaaagttgaa gaagtctttg
     2581 aatgtggcta aatctgaatt tgaccgtgat gcagccatgc aacgtaagtt ggaaaagatg
     2641 gctgatcaag ctatgaccca aatgtataaa caggctagat ctgaggacaa gagggcaaaa
     2701 gttactagtg ctatgcagac aatgcttttc actatgctta gaaagttgga taatgatgca
     2761 ctcaacaaca ttatcaacaa tgcaagagat ggttgtgttc ccttgaacat aatacctctt
     2821 acaacagcag ccaaactaat ggttgtcata ccagactata acacatataa aaatacgtgt
     2881 gatggtacaa catttactta tgcatcagca ttgtgggaaa tccaacaggt tgtagatgca
     2941 gatagtaaaa ttgttcaact tagtgaaatt agtatggaca attcacctaa tttagcatgg
     3001 cctcttattg taacagcttt aagggccaat tctgctgtca aattacagaa taatgagctt
     3061 agtcctgttg cactacgaca gatgtcttgt gctgccggta ctacacaaac tgcttgcact
     3121 gatgacaatg cgttagctta ctacaacaca acaaagggag gtaggtttgt acttgcactg
     3181 ttatccgatt tacaggattt gaaatgggct agattcccta agagtgatgg aactggtact
     3241 atctatacag aactggaacc accttgtagg tttgttacag acacacctaa aggtcctaaa
     3301 gtgaagtatt tatactttat taaaggatta aacaacctaa atagaggtat ggtacttggt
     3361 agtttagctg ccacagtacg tctacaagct ggtaatgcaa cagaagtgcc tgccaattca
     3421 actgtattat ctttctgtgc ttttgctgta gatgctgcta aagcttacaa agattatcta
     3481 gctagtgggg gacaaccaat cactaattgt gttaagatgt tgtgtacaca cactggtact
     3541 ggtcaggcaa taacagttac accggaagcc aatatggatc aagaatcctt tggtggtgca
     3601 tcgtgttgtc tgtactgccg ttgccacata gatcatccaa atcctaaagg attttgtgac
     3661 ttaatagact ggccccctga atctccagac aaccaatatc acttaaataa gtgatagtct
     3721 taatactagt ttttagacta gtcattggag aacagatgat tgatgtctta gggccggaga
     3781 aacgcagacg gcgtaccaca caggaaaaga tcgcaattgt tcagcagagc tttgaaccgg
     3841 ggatgacggt ctccctcgtt gcccggcaac atggtgtagc agccagccag ttatttctct
     3901 ggcgtaagca ataccaggaa ggaagtctta ctgctgtcgc cgccggagaa caggttgttc
     3961 ctgcctctga acttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca
     4021 agaaaacgat ggaaaatgaa ctcctcaaag aagccgttga atatggacgg gcaaaaaagt
     4081 ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct
     4141 ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatgg
     4201 ccgccgcagt cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg
     4261 agagctgcca acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact
     4321 tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct
     4381 gttgcttgag cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc
     4441 cgtgaaagaa agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg
     4501 agagagactg cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc
     4561 ggtcactacc ggcggcttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga
     4621 acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg ataatggttc
     4681 atgctaccgg gctaatgaaa cacgccagtt cgcccggatg ttgggacttg aaccgaagaa
     4741 cacggcggtg cggagtccgg agagtaacgg aatagcagag agcttcgtga aaacgataaa
     4801 gcgtgactac atcagtatca tgcccaaacc agacgggtta acggcagcaa agaaccttgc
     4861 agaggcgttc gagcattata acgaatggca tccgcatagt gcgctgggtt atcgctcgcc
     4921 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
     4981 ataggggcaa atccacttaa aaggtaagta tgtacaaata cctacaactt gtgctaatga
     5041 ccctgtgggt tttacactta aaaacacagt ctgtaccgtc tgcggtatgt ggaaaggtta
     5101 tggctgtagt tgtgatcaac tccgcgaacc catgcttcag tcagctgatg cacaatcgtt
     5161 tttaaacggg tttgcggtgt aactagcata accccttggg gcctctaaac gggtcttgag
     5221 gggttttttg catcactgac catttaaatc atacctgacc tccatagcag aaagtcaaaa
     5281 gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa
     5341 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     5401 agctaaaatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5461 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5521 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5581 acgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5641 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5701 tgacttggtt gagtactcac cagtcacaga aaagcatctc acggatggca tgacagtaag
     5761 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctggc
     5821 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5881 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5941 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6001 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggatcact
     6061 tctgcgctcg gccctcccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6121 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gcatcgtagt
     6181 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6241 aggtgcctca ctgattaagc attggtaagt gaccaaacag gaaaaaaccg cccttaacat
     6301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     6361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     6421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga tgagggccca aatgtaatca
     6481 cctggctcac cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc
     6541 ccctgacgag catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact
     6601 ataaagatac caggcgttta cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6961 aagaacagta tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt
     7021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag
     7141 gcccacccgt gaaggtgagc cagtgagttg attgcagtcc agttacgctg gagtcaagct
     7201 aatacgactc actatagaaa attgttaata attggttgaa gcagttaatt aaagttacac
     7261 ttgtgttcct ttttgttgct gctattttct atttaataac acctgttcat gtcatgtcta
     7321 aacatactga cttttcaagt gaaatcatag gatacaaggc tattgatggt ggtgtcactc
     7381 gtgacatagc atctacagat acttgttttg ctaacaaaca tgctgatttt gacacatggt
     7441 ttagccagcg tggtggtagt tatactaatg acaaagcttg cccattgatt gctgcagtca
     7501 taacaagaga agtgggtttt gtcgtgcctg gtttgcctgg cacgatatta cgcacaacta
     7561 atggtgactt tttgcatttc ttacctagag tttttagtgc agttggtaac atctgttaca
     7621 caccatcaaa acttatagag tacactgact ttgcaacatc agcttgtgtt ttggctgctg
     7681 aatgtacaat ttttaaagat gcttctggta agccagtacc atattgttat gataccaatg
     7741 tactagaagg ttctgttgct tatgaaagtt tacgccctga cacacgttat gtgctcatgg
     7801 atggctctat tattcaattt cctaacacct accttgaagg ttctgttaga gtggtaacaa
     7861 cttttgattc tgagtactgt aggcacggca cttgtgaaag atcagaagct ggtgtttgtg
     7921 tatctactag tggtagatgg gtacttaaca atgattatta cagatcttta ccaggagttt
     7981 tctgtggtgt agatgctgta aatttactta ctaatatgtt tacaccacta attcaaccta
     8041 ttggtgcttt ggacatatca gcatctatag tagctggtgg tattgtagct atcgtagtaa
     8101 catgccttgc ctactatttt atgaggttta gaagagcttt tggtgaatac agtcatgtag
     8161 ttgcctttaa tactttacta ttccttatgt cattcactgt actctgttta acaccagttt
     8221 actcattctt acctggtgtt tattctgtta tttacttgta cttgacattt tatcttacta
     8281 atgatgtttc ttttttagca
//
