LOCUS       sequence-307337-        4756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4756)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046677
FEATURES             Location/Qualifiers
     source          1..4756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..428
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(522..540)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             891..1694
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      1777..2759
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      2837..2866
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2888..3475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3487..3514)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3538..3729)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(3730..4521,4522..4590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4353..4372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4591..4693)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4694..4725)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggtcagttag agcggccgcc ccacacacca tagcttcaaa atgtttctac tcctttttta
       61 ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca
      121 gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt
      181 ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa
      241 tttttatcac gtttcttttt cttgaaaatt tttttttttg atttttttct ctttcgatga
      301 cctcccattg atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt
      361 ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc atagcaatct
      421 aatctaagtt ttaattacaa aatggctgat gcacaatcgt ttttaaacgg gtttgcgtaa
      481 acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc
      541 cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
      601 cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt
      661 ttcttttttt tctgtacaaa gcggccgccg aaccactagt gcactgcagt acacggtttc
      721 cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      781 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      841 tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag
      901 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      961 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     1021 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     1081 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     1141 acaatttttt actcttcgag gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     1201 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     1261 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     1321 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     1381 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     1441 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1501 tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1561 tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1621 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1681 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1741 gagcttcaat ttaattatat cagttattac ccgagtaacg aagcatctgt gcttcatttt
     1801 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     1861 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     1921 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     1981 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     2041 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     2101 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     2161 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     2221 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     2281 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     2341 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     2401 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     2461 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     2521 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     2581 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     2641 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     2701 gtgcgtcatc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc
     2761 tagagaatag gaacttcccg agcggccgcg tgttacaagc ttgactccag cgtaactgga
     2821 ctgcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag
     2881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccaggggtaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgca gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatca
     3541 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     3601 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     3661 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     3721 tttggtcact taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatgcggg agggcttacc
     3841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3901 agcaataaac cagccagccg ggagggccga gcgcagaagt gatcctgcaa ctttatccgc
     3961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgcc agaagtaagt tggccgcagt
     4201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtgag
     4261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4381 aaaagtgctc atcattggaa aacgttcttc ggggcgtaaa ctctcaagga tcttaccgct
     4441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4561 aagggcgaca cggaaatgtt gaatactcat tttagcttcc ttagctcctg aaaatctcga
     4621 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     4681 tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
     4741 caggtatgat ttaaat
//
