LOCUS       sequence-307355-        5761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 endoRNAse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046623
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..435
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(1534..1552)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             1903..2706
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      2789..3771
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      3849..3878
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3900..4487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4499..4526)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4550..4741)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(4742..5533,5534..5602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5365..5384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5603..5705)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(5706..5737)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tttaaatggt cagttagagc ggccgcccca cacaccatag cttcaaaatg tttctactcc
       61 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      121 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      181 taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      241 ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt
      301 tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt
      361 ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata
      421 gcaatctaat ctaagtttta attacaaaat gagcctggaa aacgttgcct tcaatgtagt
      481 gaacaaagga cactttgatg ggcaacaggg tgaggttcct gtctccatta taaataacac
      541 cgtttatact aaggtcgacg gcgttgatgt ggaattgttc gaaaataaga cgacattacc
      601 cgtcaacgtt gcatttgaac tttgggcgaa gcgtaacatc aaaccagtcc cagaagttaa
      661 gattttgaac aatttgggtg ttgacatcgc tgctaatacc gtcatttggg attacaagag
      721 agatgctccg gcccatatct caactatagg tgtctgctcg atgactgaca ttgctaaaaa
      781 gccaaccgag actatctgtg ctccattaac cgttttcttt gacggtagag tagatggtca
      841 agtcgacctc ttcagaaacg caaggaacgg tgttttgatt acagaaggta gtgttaaagg
      901 tctacaacct tctgtaggtc caaagcaagc gtccttgaac ggcgtcacgt tgattggcga
      961 agccgttaag actcaattca attactataa gaaggtcgat ggtgttgtgc aacaattacc
     1021 agaaacttac ttcactcagt ctcgcaattt gcaagaattt aaaccacgat ctcaaatgga
     1081 aatcgatttt cttgaactag ctatggacga gttcatcgaa cgttacaagt tggaaggtta
     1141 tgcttttgaa cacattgtct acggtgactt ctctcattcc cagctgggtg gtttacactt
     1201 gctaattgga ctggctaaaa gattcaagga atcaccattc gagttggaag attttatccc
     1261 tatggactct accgttaaaa actacttcat cactgatgct caaaccggtt cgagtaagtg
     1321 tgtttgttcc gtcattgact tgttactaga tgatttcgta gaaataatta agtctcaaga
     1381 cttgtcagtt gtttccaagg ttgtcaaggt aaccattgac tatacggaaa tctcatttat
     1441 gttgtggtgc aaagacggtc atgttgaaac cttctacccg aaattgcaat aaacaggccc
     1501 cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     1561 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     1621 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     1681 tttctgtaca aagcggccgc cgaaccacta gtgcactgca gtacacggtt tccttgaaat
     1741 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
     1801 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
     1861 caactgcaca gaacaaaaac gtgcaggaaa cgaagataaa tcatgtcgaa agctacatat
     1921 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
     1981 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
     2041 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
     2101 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
     2161 ttactcttcg aggacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
     2221 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
     2281 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
     2341 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
     2401 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     2461 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     2521 gatgacaagg gagatgcatt gggtcaacag tatagaaccg tggatgatgt ggtttctaca
     2581 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     2641 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     2701 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     2761 atttaattat atcagttatt acccgagtaa cgaagcatct gtgcttcatt ttgtagaaca
     2821 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     2881 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     2941 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     3001 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     3061 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     3121 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     3181 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     3241 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     3301 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     3361 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     3421 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     3481 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     3541 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     3601 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     3661 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtca
     3721 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     3781 aggaacttcc cgagcggccg cgtgttacaa gcttgactcc agcgtaactg gactgcaatc
     3841 aactcactgg ctcaccttca cgggtgggcc tttcttcggt agaaaatcaa aggatcttct
     3901 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3961 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaggta actggcttca
     4021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4381 ttccaggggt aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4441 agcatcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4501 cagaaaggcc cacccgaagg tgagccaggt gattacattt gggccctcat cagaggtttt
     4561 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     4621 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     4681 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     4741 cttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4801 tagttgcctg actccccgtc gtgtagataa ctacgatgcg ggagggctta ccatctggcc
     4861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4921 accagccagc cgggagggcc gagcgcagaa gtgatcctgc aactttatcc gcctccatcc
     4981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5161 cggttagctc cttcggtcct ccgatcgttg ccagaagtaa gttggccgca gtgttatcac
     5221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgtg agatgctttt
     5281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5401 tcatcattgg aaaacgttct tcggggcgta aactctcaag gatcttaccg ctgttgagat
     5461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5581 cacggaaatg ttgaatactc attttagctt ccttagctcc tgaaaatctc gataactcaa
     5641 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     5701 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
     5761 a
//
