LOCUS       sequence-307392-       12224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse IFT88-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12224)
  TITLE     lentiviral expression plasmids for ciliary genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-mIFT88-EGFP
FEATURES             Location/Qualifiers
     source          1..12224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2367..3545
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2598..3536
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3493..3513
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5951..5953,5954..5956,5957..6670)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5951..5953,5954..5956,5957..6667)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5996..6017)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6257..6276)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6604..6625
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6680..7268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6733..6753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7151..7162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7792..7972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7998..8015)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8004..8228
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8274..8702
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8361..8380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8571..8592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8711..8731)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8716..9045
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8896..9031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8958..8977
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9093..9140
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9159..9533
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9663..9784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9700..9719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9754..9773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9833..9849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9833..9849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9846..9868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9857..9873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9881..9887,9888..9905,9906..9911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9926..9947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10064..10081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10235..10823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10315..10334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10994..11785,11786..11854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11617..11636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11855..11959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12034..12053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     2401 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     2461 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2521 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2581 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2641 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2701 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2761 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2821 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2881 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2941 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3001 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3061 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3121 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3181 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3241 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3301 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3361 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3421 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3481 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3541 cgtgaggaat tgggccatta cggccaccat ggaaaatgtt catctggcac cagaaacaga
     3601 tgaggacgac ctttactctg gtttcaacga ctacaaccca gcctatgata ctgaggagtt
     3661 ggagaatgac acgggttttc agcaagcagt gagaaccagt catggcagaa gacctcccgt
     3721 aactgctaaa ataccaagca cagcagtcag cagacccata gctactggat atgggtccaa
     3781 gacatccctg acatcatcaa tgggaagacc gatgacaggg acaattcagg atggagttgc
     3841 tcgaccgatg acagcagtga gagcagctgg cttttccaag gcagctttgc gaggctctgc
     3901 atttgacccc cttggtcagt ccaggggccc tgctcctcct ttggaagcca aaaatgaaga
     3961 cagtccagaa gaaaaaatta gacagttgga gaagaaagta aatgagttgg tggaagaaag
     4021 ctgtattgcc aacagttgtg gagacttaaa actggcctta gagaaggcaa aagatgcagg
     4081 aagaaaagag agagtcctgg tgagacaacg agagcaagtc acaagtccag aaaatatcaa
     4141 cttggactta acctactccg ttcttttcaa cttggccagt cagtattctg ctaatgaaat
     4201 gtatgcagaa gcactgaaca cataccaagt tatagtgaaa aacaagatgt ttagcaatgc
     4261 aggaagactg aaagtgaata tgggaaatat ttatttaaag cagagaaatt attccaaagc
     4321 cattaagttc taccgaatgg ccttagatca gatcccaagt gtccataaag aaatgaggat
     4381 caaaataatg cagaacattg gaatcacatt tataaaaact ggtcagtact cagatgccat
     4441 caactcattt gagcacatca tgagcatggc cccgagcctg aaggcaggct tcaacctcat
     4501 ccttagttgt tttgccattg gagatcggga gaaaatgaag aaggcattcc aaaaattaat
     4561 tgctgttccg ttagaaattg atgaagatga taaatatatc tccccaagtg atgatcccca
     4621 tactaacttg ctgattgaag ctataaaaaa tgaccacctt aggcaaatgg aacgtgaaag
     4681 gaaagccatg gcagaaaaat acatcatgac agctgcaaaa ctcattgctc ctgtgatcga
     4741 agcgtctttt gctgtgggtt ataactggtg tgtggaagta gtgaaagcct ctcagtatgt
     4801 ggagctggcc aacgacctgg agattaacaa agcaattact tacttgagac aaaaggactt
     4861 taaccaagct gtagacacat tgaaaatgtt tgagaagaag gacagtagag tgaagagtgc
     4921 agctgcgacc aacctctcgt tcctgtatta tctggaaaac gaatttgccc aagccagcag
     4981 ctatgcagat ttagctgtga actcggatag atatagcccg tcagctctca ctaataaagg
     5041 gaacacagtt tttgcaaatg gtgattatga gaaggcagct gagttctata aagaggccct
     5101 gagaaacgac tcttcctgta ctgaagcact gtataacatt ggcctcacct ataagaagct
     5161 gaaccgtctg gatgaagccc tggactcctt cctgaaactg cacgcgatcc ttcggaacag
     5221 cgctcaagtt ctttgccaga tagcaaacat atatgagtta atggaagatc ccaatcaagc
     5281 tattgaatgg ctgatgcagt tgatcagcgt tgttccaact gattcccaag ctctgtctaa
     5341 actgggagag ttatacgata gtgagggaga caagtcccag gcattccaat attactatga
     5401 gtcatacagg tatttccctt ctaacattga agtcattgaa tggcttggag cttattacat
     5461 tgatacacag ttctgcgaga aagccattca gtactttgaa agagcttccc ttatacaacc
     5521 cacacaagtg aagtggcagc tgatggtagc tagctgcttt agaagaagtg gtaactacca
     5581 aaaggcatta gatacttata aagagattca caggaaattt ccggagaatg ttgaatgttt
     5641 gcgtttcttg gttcgtctct gcacagatat tggattaaaa gaagttcaag aatatgccac
     5701 aaaactcaag aggttggaaa aaatgaaaga aatgagggaa cagcgcataa aatcgggcag
     5761 agacagcagc gggggttctc gcagcaaacg agaggggagt gctggcagtg atgcctccta
     5821 cgtggatcca ctgggccctc agatagaaag accaaaaact gcagccaaga aaagaattga
     5881 cgaggatgat tttgctgatg aagagttagg agatgacttg ctcccagaag gccgcctcgg
     5941 ccccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6001 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6061 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6121 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     6181 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6241 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6301 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6361 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     6421 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     6481 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6541 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6601 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6661 gtacaagtaa agcggccgca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7081 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7261 ctccccgcct gcctgcaggt ttgtcgagac ctagaaaaac atggagcaat cacaagtagc
     7321 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7381 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7441 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7501 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7561 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7621 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7681 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7741 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7801 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7861 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7921 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7981 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     8041 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8101 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8161 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8221 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     8281 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8341 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8401 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8461 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8521 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8581 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8641 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8701 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8761 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8881 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8941 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     9001 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     9061 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     9121 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     9181 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     9241 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     9301 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     9361 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     9421 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     9481 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     9541 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9601 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9661 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9721 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9781 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    11341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    12001 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    12061 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    12121 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    12181 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
