LOCUS       sequence-30740-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB.
  TITLE     Merkel cell polyomavirus and two previously unknown polyomaviruses 
            are chronically shed from human skin.
  JOURNAL   Cell Host Microbe. 2010 Jun 25;7(6):509-15.
  PUBMED    20542254
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2010-06-25"; volume: "7(6)"; pages: "509-15"
COMMENT     Sequence Label: pFunnyfarm
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             375..680
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     559..578
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gggggcgtaa acgccgcgtg gatccggctt actaaaagcc
       61 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
      121 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
      181 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
      241 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
      301 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
      361 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
      421 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
      481 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
      541 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
      601 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
      661 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgat
      721 acagtagaaa ttacagaaac tttatcacgt ttagtaagta tagaggctga aaatccagat
      781 gaagccgaac gacttgtaag agaaaagtat aagagttgtg aaattgttct tgatgcagat
      841 gattttcagg actatgacac tagcgtatat gaataggtag atg
//
