LOCUS       sequence-30744-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Mizushima N, Sugita H, Yoshimori T, Ohsumi Y
  TITLE     A new protein conjugation system in human. The counterpart of the 
            yeast Apg12p conjugation system essential for autophagy.
  JOURNAL   J Biol Chem. 1998 Dec 18. 273(51):33889-92.
  PUBMED    9852036
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Dec 18. 273(51):33889-92."
COMMENT     Sequence Label: pCI-neo-HA-hApg12
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..98
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    621..742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(632..651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     686..705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatctttta cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact
       61 atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgcaga tctagagcgg
      121 aggagccgca gtctgtgttg cagcttccta cttcaattgc tgctggaggg gaaggactta
      181 cggatgtctc cccagaaaca accaccccgg agcccccgtc ttccgctgca gtttccccgg
      241 gaacagagga acctgctggc gacaccaaga aaaaaattga cattttgcta aaggctgtgg
      301 gagacactcc tattatgaaa acaaagaagt gggcagtaga gcgaacacga accatccaag
      361 gactcattga cttcatcaaa aagtttctta aacttgtggc ctcagaacag ttgtttattt
      421 atgtgaatca gtcctttgct ccttccccag accaagaagt tggaactctc tatgagtgtt
      481 ttggcagtga tggtaaactg gttttacatt actgcaagtc tcaggcgtgg ggatgagaat
      541 tcacgcgtgg tacctctagc tagagtcgac ccgggcggcc gcttcccttt agtgagggtt
      601 aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      721 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      781 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg
      841 ataaggatcg atccgggctg gcgtaatagc g
//
