LOCUS       sequence-307446-        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers 
            J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice
            MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG
  TITLE     Specific Labeling of Stem Cell Activity in Human Colorectal 
            Organoids Using an ASCL2-Responsive Minigene.
  JOURNAL   Cell Rep. 2018 Feb 6;22(6):1600-1614. doi: 
            10.1016/j.celrep.2018.01.033.
  PUBMED    29425513
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-6- 6"; volume: "22"; issue: "6"; pages: "1600-1614"
COMMENT     Sequence Label: pLV 4xSTAR-mTagBFP2-blast
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1150..1702
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1294..1311)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1521..1540
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1729..2127
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gtgaaaaggc aggtgcaaag caaatcctat cccagcttct agctaggtga aaaggcaggt
       61 gcaaagcaaa tcctatccca gcttctagct agggtgaaaa ggcaggtgca aagcaaatcc
      121 tatcccagct tctagctagg tgaaaaggca ggtgcaaagc aaatcctatc ccagcttcta
      181 gctagcggcg cgccggatcc ggaggggaca agtggaggga aagtggggcc gggcgggggg
      241 tgcctgggaa gccaggctgc gctgacgtca ctgggcgcgc aattcgggct ggagcgcttt
      301 aaaaaacgag cgtgcaagca gagaggctgc tccacaccgc tcaggccgcg accatggtgt
      361 ctaagggcga agaactgatc aaagaaaata tgcatatgaa actgtatatg gaaggaacag
      421 tggataatca ccattttaaa tgtaccagcg aaggagaggg aaaaccttat gaaggaacac
      481 agacaatgcg aattaaagtc gtggaaggag ggcccctccc ttttgccttt gatattctgg
      541 ctacttcctt tctctatgga tccaagacat ttattaatca cacacaggga attcctgatt
      601 ttttcaaaca gagctttcct gaagggttta cctgggaacg ggtgacaacc tatgaggatg
      661 gcggggtgct gacagctaca caggatacat ccctccagga tgggtgtctc atttataatg
      721 tcaaaattcg gggcgtgaat tttaccagca atggacccgt gatgcagaaa aagaccctcg
      781 gatgggaagc ctttacagaa acgctgtatc ctgctgatgg agggctggag gggaggaacg
      841 atatggccct gaaactcgtg ggaggcagcc atctgattgc aaatgccaaa acaacctata
      901 ggagcaaaaa gcctgctaag aatctcaaaa tgcctggagt ctattatgtg gattacaggc
      961 tggagaggat taaagaagcc aacaacgaaa catatgtcga acagcatgaa gtggcagtgg
     1021 ccaggtattg tgatctcccc tccaagctgg gccataagct ttctagacag cgctcaccgg
     1081 tcgttaacat taagctagcc tagtgccatt tgttcagtgg cccgggaatt ccgcccctct
     1141 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     1201 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     1261 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     1321 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     1381 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     1441 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     1501 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     1561 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     1621 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     1681 tttgaaaaac acgatgataa tatggatatg gccacaacgg tcgccaccat ggccaagcct
     1741 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     1801 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     1861 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     1921 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     1981 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     2041 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     2101 ccctctggtt atgtgtggga gggctaa
//
