LOCUS       sequence-30745-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Mizushima N, Sugita H, Yoshimori T, Ohsumi Y
  TITLE     A new protein conjugation system in human. The counterpart of the 
            yeast Apg12p conjugation system essential for autophagy.
  JOURNAL   J Biol Chem. 1998 Dec 18. 273(51):33889-92.
  PUBMED    9852036
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Dec 18. 273(51):33889-92."
COMMENT     Sequence Label: pCI-neo-Myc-hApg12
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..58
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             65..94
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    614..735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(625..644)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     679..698
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa
       61 cggtgaacaa aaattaatct cagaagaaga cttgaacgga tcctctagag cggaggagcc
      121 gcagtctgtg ttgcagcttc ctacttcaat tgctgctgga ggggaaggac ttacggatgt
      181 ctccccagaa acaaccaccc cggagccccc gtcttccgct gcagtttccc cgggaacaga
      241 ggaacctgct ggcgacacca agaaaaaaat tgacattttg ctaaaggctg tgggagacac
      301 tcctattatg aaaacaaaga agtgggcagt agagcgaaca cgaaccatcc aaggactcat
      361 tgacttcatc aaaaagtttc ttaaacttgt ggcctcagaa cagttgttta tttatgtgaa
      421 tcagtccttt gctccttccc cagaccaaga agttggaact ctctatgagt gttttggcag
      481 tgatggtaaa ctggttttac attactgcaa gtctcaggcg tggggatgag aattcacgcg
      541 tggtacctct agctagagtc gacccgggcg gccgcttccc tttagtgagg gttaatgctt
      601 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      661 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      721 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcaggggaga
      781 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg t
//
