LOCUS       sequence-307457-        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear-targeted FRET-based cAMP indicator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Sample V, DiPilato LM, Yang JH, Ni Q, Saucerman JJ, Zhang J
  TITLE     Regulation of nuclear PKA revealed by spatiotemporal manipulation of
            cyclic AMP.
  JOURNAL   Nat Chem Biol. 2012 Feb 26;8(4):375-82. doi: 10.1038/nchembio.799.
  PUBMED    22366721
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-02-26- 26"; volume: "8"; 
            issue: "4"; pages: "375-82"
COMMENT     Sequence Label: pcDNA3-ICUE3-NLS
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(979..987,988..1002)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(4090..4092,4093..4095,4096..4608)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             4681..4701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4729..4747)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4730..4747)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4767..4784)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4773..4997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5043..5471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5130..5149)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5340..5361
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5480..5500)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5485..5814
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5665..5800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5727..5746
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5881..6675
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5935..5954)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6545..6564
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6849..6970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6886..6905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6940..6959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7019..7035)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7019..7035)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7032..7054)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7043..7059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7067..7073,7074..7091,7092..7097))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7112..7133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7250..7267)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7421..8009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7501..7520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8180..8971,8972..9040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8803..8822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9041..9145)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1441 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1501 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1621 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaaggctgg tacccccgtg
     1741 ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc ccagcggggg
     1801 cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac ggatgaagag
     1861 ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct ctccaactcg
     1921 gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc agggaccgtg
     1981 ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg atctgtcaac
     2041 gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga ttttggacag
     2101 ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga agacaactgt
     2161 catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt ggaggcaaag
     2221 accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc ctctcagggc
     2281 gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat gtctggcacc
     2341 ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag tgctcatgac
     2401 caaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat gcccagcgcc
     2461 caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg cagcgagcag
     2521 gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt cagccagtgg
     2581 gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt cctccagaaa
     2641 ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga gcagtggcca
     2701 gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc tcagatgaag
     2761 gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg cagcagctgt
     2821 gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca ctcagtgttg
     2881 accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt ggcccaggag
     2941 gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga tgccattggc
     3001 ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg tctctttgtt
     3061 gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg gcccactgtg
     3121 ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca gctgacggac
     3181 cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta tgtgctgggc
     3241 ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg ccgcttcaat
     3301 gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg cccccgggcc
     3361 cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa gaatctcaat
     3421 tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccacct agcccacacc
     3481 tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag gctgctggac
     3541 ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc tcctgtcatc
     3601 cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa ccacacacta
     3661 gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagtcgc gcggatgctg
     3721 caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg agtttcccac
     3781 ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc cctgagcacc
     3841 cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga caaccagcgg
     3901 gaactctccc gcctctcccg agagctggag ccagagctca tgcccgacaa ccactacctg
     3961 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4021 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggaggtacc
     4081 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     4261 tggcccaccc tcgtgaccac cctgggctac ggccttcagt gcttcgcccg ctaccccgac
     4321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     4621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctggaattc
     4681 cccaaaaaga agagaaaggt ggaagacgca taatctagag ggccctattc tatagtgtca
     4741 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4801 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4861 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4921 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4981 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5041 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5101 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5161 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5221 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5281 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5341 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5401 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5461 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5641 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5701 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5761 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5821 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5881 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5941 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6001 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6061 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6121 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6181 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6241 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6301 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6361 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6421 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6481 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6541 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6601 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6661 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6721 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6781 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6841 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6961 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7021 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7081 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7141 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7201 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7321 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
