LOCUS       sequence-307462-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); 
            nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to 
            monitor cAMP dynamics within AKAP95 nanodomain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Clister T, Greenwald EC, Baillie GS, Zhang J
  TITLE     AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for 
            Regulating Nuclear PKA.
  JOURNAL   Cell Chem Biol. 2019 Jun 20;26(6):885-891.e4. doi: 
            10.1016/j.chembiol.2019.03.003. Epub 2019 Apr 11.
  PUBMED    30982750
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2019.03.003"; journalName: "Cell Chem Biol"; 
            date: "2019-06-20- 20"; volume: "26"; issue: "6"; pages: 
            "885-891.e4"
COMMENT     Sequence Label: pcDNA3-FKBP-ICUE3-NLS
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(907..909,910..1230)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             join(1300..1302,1303..1305,1306..2016)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1300..1302,1303..1305,1306..2016)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1300..1302,1303..1305,1306..2016)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(4381..4383,4384..4386,4387..4899)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             4972..4992
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5020..5038)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5021..5038)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5058..5075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5064..5288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5334..5762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5421..5440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5631..5652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5771..5791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5776..6105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5956..6091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6018..6037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6172..6966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6226..6245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6836..6855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7140..7261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7177..7196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7231..7250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7310..7326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7310..7326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7323..7345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7334..7350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7358..7364,7365..7382,7383..7388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7403..7424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7541..7558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7712..8300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7792..7811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8471..9262,9263..9331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9094..9113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9332..9436)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
      961 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     1021 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     1081 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     1141 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     1201 gtcttcgatg tggagcttct aaaactggaa agtgctggtg gtagtgctgg tggtagtgct
     1261 ggtggtagtg ctggtggtag tgctggtggt ggggatccca tggtgagcaa gggcgaggag
     1321 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg
     1381 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1441 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1501 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1561 gccatgcccg aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac
     1621 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1681 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     1741 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag
     1801 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1861 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1921 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1981 gccgggatca ctcacggcat ggacgagctg tacaaggctg gtacccccgt gggaactcat
     2041 gagatggagg aggagttggc cgaagctgtg gccctgctct cccagcgggg gcctgacgcc
     2101 ctgctcactg tggcacttcg aaagccccca ggtcagcgca cggatgaaga gctggacctc
     2161 atctttgagg agctgctgca catcaaggct gtggcccacc tctccaactc ggtgaagcga
     2221 gaattagcgg ctgttctgct ctttgaacca cacagcaagg cagggaccgt gttgttcagc
     2281 cagggggaca agggcacttc gtggtacatt atctggaagg gatctgtcaa cgtggtgacc
     2341 catggcaagg ggctggtgac caccctgcat gagggagatg attttggaca gctggctctg
     2401 gtgaatgatg caccccgggc agccaccatc atcctgcgag aagacaactg tcatttcctg
     2461 cgtgtggaca agcaggactt caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg
     2521 ctggaagaac atggcaaagt ggtgctggtg ctggagagag cctctcaggg cgccggccct
     2581 tcccgacccc caaccccagg caggaaccgg tatacagtga tgtctggcac cccagagaag
     2641 atcctagagc ttctgttgga ggccatggga ccagattcca gtgctcatga ccaaacagag
     2701 acattcctca gcgacttcct cctgacccac agggtcttca tgcccagcgc ccaactctgc
     2761 gctgcccttc tgcaccactt ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc
     2821 acctacgtct gcaacaagag gcagcagatc ttgcggctgg tcagccagtg ggtggccctg
     2881 tatggctcca tgctccacac tgaccctgtg gccaccagct tcctccagaa actctcagac
     2941 ctggtgggca gggacacccg actcagcaac ctgctgaggg agcagtggcc agagaggcgg
     3001 cgatgccaca ggttggagaa tggctgtggg aatgcatctc ctcagatgaa ggcccggaac
     3061 ttgcctgttt ggctccccaa ccaggacgag ccccttcctg gcagcagctg tgccatccaa
     3121 gttggggata aagtccccta tgacatctgc cggccagacc actcagtgtt gaccctgcag
     3181 ctgcctgtga cagcctccgt gagagaggtg atggcagcgt tggcccagga ggatggctgg
     3241 accaaggggc aggtgctggt gaaggtcaat tctgcaggtg atgccattgg cctgcagcca
     3301 gatgcccgtg gtgtggccac atctctgggg ctcaatgagc gtctctttgt tgtcaaccca
     3361 caggaagcgc atgagctgat cccacaccct gaccagctgg ggcccactgt gggctctgct
     3421 gaggggctgg acctggtgag tgccaaggac ctggcaggcc agctgacgga ccacgactgg
     3481 agcctcttca acagtatcca ccaggtggag ctgatccact atgtgctggg cccccagcat
     3541 ctgcgggatg tcaccaccgc caacctggag cgcttcatgc gccgcttcaa tgagctgcag
     3601 tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc
     3661 aggaagttca ttaagctggc ggcccacctc aaggagcaga agaatctcaa ttccttcttt
     3721 gccgtcatgt ttggcctcag caactcggcc atcagccacc tagcccacac ctgggagcgg
     3781 ctgccccaca aagtccggaa gctgtactcc gccctcgaga ggctgctgga cccctcatgg
     3841 aaccaccggg tataccgact ggccctcgcc aagctctccc ctcctgtcat ccccttcatg
     3901 ccccttcttc tcaaagacat gaccttcatt catgagggaa accacacact agtggagaat
     3961 ctcatcaact ttgagaagat gagaatgatg gccagagtcg cgcggatgct gcaccactgc
     4021 cgaagccaca accctgtgcc tctctcacca ctcagaagcc gagtttccca cctccacgag
     4081 gacagccagg tggcgaggat ttccacatgc tcggagcagt ccctgagcac ccggagtcca
     4141 gccagcacct gggcttatgt ccagcagctg aaggtcattg acaaccagcg ggaactctcc
     4201 cgcctctccc gagagctgga gccagagctc atgcccgaca accactacct gagctaccag
     4261 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4321 accgccgccg ggatcactct cggcatggac gagctgtaca agggaggtac cggtggatct
     4381 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4441 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4501 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4561 ctcgtgacca ccctgggcta cggccttcag tgcttcgccc gctaccccga ccacatgaag
     4621 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4681 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4741 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4801 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     4861 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     4921 gaccactacc agcagaacac ccccatcggc gacggccccg tgctggaatt ccccaaaaag
     4981 aagagaaagg tggaagacgc ataatctaga gggccctatt ctatagtgtc acctaaatgc
     5041 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
