LOCUS       sequence-307480-        6131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A shuttle vector between E. coli and magnetotactic bacteria (e.g. 
            Magnetospirillum) containing a TED module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Kondo T, Yumura S
  TITLE     An improved molecular tool for screening bacterial colonies using 
            GFP expression enhanced by a Dictyostelium sequence.
  JOURNAL   Biotechniques. 2020 Feb;68(2):91-95. doi: 10.2144/btn-2019-0127. 
            Epub 2019 Dec 11.
  PUBMED    31825246
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.2144/btn-2019-0127"; journalName: "Biotechniques"; date: 
            "2020-02"; volume: "68"; issue: "2"; pages: "91-95"
COMMENT     Sequence Label: pMYU1A-TED
FEATURES             Location/Qualifiers
     source          1..6131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..28
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20..37
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     21..37
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(993..1009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(993..1009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1006..1028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1017..1033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1041..1047,1048..1065,1066..1071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1086..1107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1224..1241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1395..1983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1475..1494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2154..2945,2946..3014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2777..2796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3015..3119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3187..3205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3243..3265)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3365..3384
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     regulatory      5428..5437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgacgcgta ttgggatatc
       61 gtcgacatac tcgagataga attcataggt accatagcgg ccgcataggg cccataggat
      121 ccataagatc ttcacttata caattcatcc atacccaagg taattcctgc ggcagtaaca
      181 aactccaaaa gaaccatgtg atcacgtttt tcattaggat ctttactcaa tttagattga
      241 gtactcaaat aatgattatc tggtaacaat actggaccat ctccaatagg tgtattttgt
      301 tggtagtgat cagctaattg aactgaacca tcctctatat tgtgtctgat tttaaaatta
      361 actttaatac cattcttttg cttatcagcc atgatgtaaa cattatgaga attgtaattg
      421 tattccaact tgtgtcctaa gatatttcca tcctccttga aatctatacc ctttaactct
      481 atacgattta caagggtatc accctcaaat tttacctctg ctctagtctt ataattacca
      541 tcatctttga agaagatggt acgctcttgt acgtatcctt ctggcatggc tgacttgaag
      601 aaatcgtgtt gtttcatatg atcagggtaa cgactaaaac attgtacacc gtaggtaaga
      661 gtagtaacca aggtaggcca gggtacaggt aactttccag ttgtacatat gaatttaaga
      721 gttaattttc catatgtagc atctccttca ccttcaccag aaactgagaa tttatgtcca
      781 tttacatctc catctaactc tactaaaata ggtactactc cagtgaaaag ctcctctcct
      841 ttactaacca tagatccacc acctcctttc ttactgaaga gagtattaac gagatctata
      901 ctgcagatac ccgggatact agtattctag aataaagctt ataaccggta tagtttaaac
      961 tgtgccttct agttgccagc ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1021 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1081 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1141 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1201 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3121 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3181 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3241 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3301 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3361 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     3421 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     3481 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3541 gctgcaaggc gattacgcga ggtcacaatt ggagatgatc cgccagcgcc gaagccatca
     3601 taactgcatt gaataattca aagtgcattg aataaggcag ggtgcataag gcgcagcctt
     3661 tcgattaact ccatttcctg cggcgacaac gtcgccgctt gcccccccga cgggggtcaa
     3721 gcgaggcaca gggacccgcc gcctcttggc ggtgggtggc cgcagcttga cgcccgaaca
     3781 ctccgccgcc caaccctgcc tgctctcctc tgttcaatga aattgaacgg aggagatgcc
     3841 tcccggcgag ggcctttctt tcttgcataa cgttcatgct acgttccaac ggttataccg
     3901 tatgagcgtc gaactgtata atcgttgaac agtcgaacca tccccccgcg ccagcgcggg
     3961 gagcagggat gggttcgggg gccggaagtt aggcaaatcg cggagccgtg gcgccctcgg
     4021 tacatccgcg aaatcggttg acactttgcc cccagaacgg ctgcaaccta ccgcttggcg
     4081 acagccgcgt gacggtactt ttccgctgtg gattcggaca cgaatatctg ttcagcagcc
     4141 agggcgcggg cgatctggcg acggccgagg ccctgatggg ccaattctgt gatccggtct
     4201 atgaccgctt ggctcatcgg cacccgagga ccgccgatac cagattggcg cacatcgccc
     4261 atggtgggtg gaaccgaggg agccggagcc ggtgcagatt tctgcacagt tggttcacgc
     4321 cgtcgttcta gcgcatcgag acgctgcacg atcacggcga gctgatcgtt gacgtattgt
     4381 cgctcctcgg actgcgcaat tgtgatcaga aattcgagga gctcggccac ggtgacgccg
     4441 cgtcgaaggg ccatagcctg tatgtggtcg cggaccgcat caggacagcg aatcgtccat
     4501 ctcgtgtctg acacattccc ggtttttgtc tgactcatcg tcgcccccaa tgtctgacag
     4561 attcgagaaa gagtcatacc ggctaacgcg aacgctgaca acgaatgtct gacaaaatga
     4621 tgattacgtc agacattgat gtctgcctaa atcgcaatag tgtctgacat ggtttttggc
     4681 gttccgtata taaaatatgc gccaaaatct agtgcgtcag ccaaacggca aacgcaacca
     4741 gttgacacca tttccggttt tatgagactg tgaaaagacc aaaggccgcc ttgcggcggc
     4801 ctaggtgatt gtggtcccgg cgaccaaggc tgcgaaaact tggcggtcca gcagcagagg
     4861 aagcccgttt gaaaggtccg gcttgcgccg gaaccaccta atgacaatag caattcgccg
     4921 cacagttttc aacacccagc ccgccatgat tcgcggcggc gttgaacgtt gaacaacaga
     4981 cgtctcaaac agacacccgc aaacgcgaac ggtatcggtt gcaggatgca gccgctgaac
     5041 tgctgccgga ttatcgggtg cggcattgcc accgctcaat tggatgtggc tgtggcgagt
     5101 tggtcagcgt tgttaccaat gggcagtcgg ccaatttcaa aggcgtgcag acctgcggct
     5161 cggtctggca ctgcccaatc tgttcggcaa aaatcagcaa tgttcggcgc ggcgagctga
     5221 acgagctgct gcgctggggg cgggagaggg agctgatccc gctgatggtg acgctgacag
     5281 gccatcacaa tgcacggatg agcctcgctg aatgtctgac ggtgatgaag aaggccaagc
     5341 agagccttca ccagtctggc ccatggaagc gccatgttgg cccggtggtc gaagggcacg
     5401 tcacggcaac ggaggtcaat cggtcacgcc gccatggctg gcacgtccat tttcacgtcc
     5461 tgatgctgat tgaggccgcc agcgaggccg aagcgattgc gctcgtgacc cgacacctcg
     5521 aacctgaatg gctccggcag cttgagcgcc aaggcagctg gggcggcgaa catgcgttag
     5581 acgtccaggg cgcagccgat gcggatcggt atgtggccaa gtggggcgcg gctgaggaat
     5641 tggctctgtc gggcgaaaag cgggcgcgcg gcgacgccga aattaagggt caaacgccgt
     5701 gggaattgtt ggccgctgcg gctgatggtg acgtccaggc tggccgcgat tttgtggagt
     5761 acgcgaccct gtttcatggc aaacgccagc tcgtttggtc gcgaggactg aaagagcgtg
     5821 tcggcctcaa ggtggtggaa gacgacgagg ccgccggcga gctggaggat gtcgcagata
     5881 gcgccatcaa cgaagtggcc gtgttgccga ggccgacttg gcgtcacatc gtccggcgca
     5941 ggctccgcgc ggatctgctg gccgccgctc gtgatgacgg attcgatggc gtcgcgcggc
     6001 tgctcaatga gcatggcatc ggcggagcta tgcccggcgg ctggcgacca atgcttagcg
     6061 aatataccga gcatattgcg atggctgcgt gactcttttg gcccaccggc ctgagggaaa
     6121 tttgcctcgc a
//
