LOCUS       sequence-307489-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. 
            Contains monomeric Venus/cpVenus-E172 homoFRET pair..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE,
            McFarland JJ, Mauban JR, Ward CW, Rizzo MA, Zhang J
  TITLE     Single-color, ratiometric biosensors for detecting signaling 
            activities in live cells.
  JOURNAL   eLife. 2018 Jul 3;7. pii: 35458. doi: 10.7554/eLife.35458
  PUBMED    29968564
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-07-3"; volume: "7. pii: 35458. doi"; pages: " 
            10.7554/eLife.3545"
COMMENT     Sequence Label: pcDNA3-Venus-cpVenus-FLARE-ICUE
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..796
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        777..795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             836..853
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             857..889
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(893..901,902..916)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(923..925,926..928,929..1639)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3854..4588
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4652..4670)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4653..4670)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4690..4707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4696..4920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4966..5394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5053..5072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5263..5284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5403..5423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5408..5737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5588..5723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5650..5669
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5804..6598
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5858..5877)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6468..6487
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6772..6893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6809..6828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6863..6882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6942..6958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6955..6977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6966..6982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6990..6996,6997..7014,7015..7020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7035..7056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7173..7190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7344..7932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7424..7443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8103..8894,8895..8963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8726..8745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8964..9068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9143..9162)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
       61 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      781 acgactcact atagggagac ccaagcttgc ggccgccacc atggtgcggg gttctcatca
      841 tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta
      901 cgacgatgac gataaggatc ccatggtgag caagggcgag gagctgttca ccggggtggt
      961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1081 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1381 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1441 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1501 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1621 catggacgag ctgtacaagc gcatgcatcc cgtgggaact catgagatgg aggaggagtt
     1681 ggccgaagct gtggccctgc tctcccagcg ggggcctgac gccctgctca ctgtggcact
     1741 tcgaaagccc ccaggtcagc gcacggatga agagctggac ctcatctttg aggagctgct
     1801 gcacatcaag gctgtggccc acctctccaa ctcggtgaag cgagaattag cggctgttct
     1861 gctctttgaa ccacacagca aggcagggac cgtgttgttc agccaggggg acaagggcac
     1921 ttcgtggtac attatctgga agggatctgt caacgtggtg acccatggca aggggctggt
     1981 gaccaccctg catgagggag atgattttgg acagctggct ctggtgaatg atgcaccccg
     2041 ggcagccacc atcatcctgc gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga
     2101 cttcaaccgt atcatcaagg atgtggaggc aaagaccatg cggctggaag aacatggcaa
     2161 agtggtgctg gtgctggaga gagcctctca gggcgccggc ccttcccgac ccccaacccc
     2221 aggcaggaac cggtatacag tgatgtctgg caccccagag aagatcctag agcttctgtt
     2281 ggaggccatg ggaccagatt ccagtgctca tgaccaaaca gagacattcc tcagcgactt
     2341 cctcctgacc cacagggtct tcatgcccag cgcccaactc tgcgctgccc ttctgcacca
     2401 cttccatgtg gagcctgcgg gtggcagcga gcaggagcgc agcacctacg tctgcaacaa
     2461 gaggcagcag atcttgcggc tggtcagcca gtgggtggcc ctgtatggct ccatgctcca
     2521 cactgaccct gtggccacca gcttcctcca gaaactctca gacctggtgg gcagggacac
     2581 ccgactcagc aacctgctga gggagcagtg gccagagagg cggcgatgcc acaggttgga
     2641 gaatggctgt gggaatgcat ctcctcagat gaaggcccgg aacttgcctg tttggctccc
     2701 caaccaggac gagccccttc ctggcagcag ctgtgccatc caagttgggg ataaagtccc
     2761 ctatgacatc tgccggccag accactcagt gttgaccctg cagctgcctg tgacagcctc
     2821 cgtgagagag gtgatggcag cgttggccca ggaggatggc tggaccaagg ggcaggtgct
     2881 ggtgaaggtc aattctgcag gtgatgccat tggcctgcag ccagatgccc gtggtgtggc
     2941 cacatctctg gggctcaatg agcgtctctt tgttgtcaac ccacaggaag cgcatgagct
     3001 gatcccacac cctgaccagc tggggcccac tgtgggctct gctgaggggc tggacctggt
     3061 gagtgccaag gacctggcag gccagctgac ggaccacgac tggagcctct tcaacagtat
     3121 ccaccaggtg gagctgatcc actatgtgct gggcccccag catctgcggg atgtcaccac
     3181 cgccaacctg gagcgcttca tgcgccgctt caatgagctg cagtactggg tggccaccga
     3241 gctgtgtctc tgccccgtgc ccggcccccg ggcccagctg ctcaggaagt tcattaagct
     3301 ggcggcccac ctcaaggagc agaagaatct caattccttc tttgccgtca tgtttggcct
     3361 cagcaactcg gccatcagcc acctagccca cacctgggag cggctgcccc acaaagtccg
     3421 gaagctgtac tccgccctcg agaggctgct ggacccctca tggaaccacc gggtataccg
     3481 actggccctc gccaagctct cccctcctgt catccccttc atgccccttc ttctcaaaga
     3541 catgaccttc attcatgagg gaaaccacac actagtggag aatctcatca actttgagaa
     3601 gatgagaatg atggccagag tcgcgcggat gctgcaccac tgccgaagcc acaaccctgt
     3661 gcctctctca ccactcagaa gccgagtttc ccacctccac gaggacagcc aggtggcgag
     3721 gatttccaca tgctcggagc agtccctgag cacccggagt ccagccagca cctgggctta
     3781 tgtccagcag ctgaaggtca ttgacaacca gcgggaactc tcccgcctct cccgagagct
     3841 ggagccagag ctcatgggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     3901 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     3961 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4021 gatcactctc ggcatggacg agctgtacaa gggaggtacc ggtggatcta tggtgagcaa
     4081 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4141 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4201 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4261 cctgggctac ggccttcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     4321 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4381 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4441 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4501 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     4561 caacttcaag atccgccaca acatcgagta agaattctgc agatatccat cacactggcg
     4621 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     4681 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4741 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4801 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4861 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4921 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4981 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5041 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5101 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5161 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5221 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5281 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5341 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5641 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5761 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6301 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6601 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6661 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6721 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6901 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7021 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7081 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7741 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8161 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8221 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8281 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8341 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8401 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8461 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8521 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8581 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8641 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8701 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8761 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8821 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8881 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8941 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9061 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9121 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9181 tgctc
//
