LOCUS       sequence-307505-        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and protien purification of a mutated RUVBL2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ju D, Zhang W, Yan J, Zhao H, Li W, Wang J, Liao M, Xu Z, Wang Z, 
            Zhou G, Mei L, Hou N, Ying S, Cai T, Chen S, Xie X, Lai L, Tang C, 
            Park N, Takahashi JS, Huang N, Qi X, Zhang EE
  TITLE     Chemical perturbations reveal that RUVBL2 regulates the circadian 
            phase in mammals.
  JOURNAL   Sci Transl Med. 2020 May 6;12(542). pii: 12/542/eaba0769. doi: 
            10.1126/scitranslmed.aba0769.
  PUBMED    32376767
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2020-05-6"; volume: "12(542). pii: 12/542/eaba0769. 
            doi"; pages: " 10.1126/scitranslmed.aba0769"
COMMENT     Sequence Label: pET-28a RUVBL2 Delete
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             108..401
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             1516..1533
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat ggctagcatg tcggactcag
      121 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca
      181 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt
      241 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat
      301 tcttgtacga cggtattagg attcaagctg atcagacccc tgaagatttg gacatggagg
      361 ataacgatat tattgaggct cacagagaac agattggtgg atccatggca accgttacag
      421 ccacaaccaa agtcccggag atccgtgatg taacaaggat tgagcgaatc ggtgcccact
      481 cccacatccg gggactgggg ctggacgatg ccttggagcc tcggcaggct tcgcaaggca
      541 tggtgggtca gctggcggca cggcgggcgg ctggcgtggt gctggagatg atccgggaag
      601 ggaagattgc cggtcgggca gtccttattg ctggccagcc gggcacgggg aagacggcca
      661 tcgccatggg catggcgcag gccctgggcc ctgacacgcc attcacagcc atcgccggca
      721 gtgaaatctt ctccctggag atgagcaaga ccgaggcgct gacgcaggcc ttccggcggt
      781 ccatcggcgt tcgcatcaag gagggtcctc caggcgtggt gcacaccgtg tccctgcacg
      841 agatcgacgt catcaactct cgcacccagg gcttcctggc gctcttctca ggtgacacag
      901 gggagatcaa gtcagaagtc cgtgagcaga tcaatgccaa ggtggctgag tggcgcgagg
      961 agggcaaggc ggagatcatc cctggagtgc tgttcatcga cgaggtccac atgctggaca
     1021 tcgagagctt ctccttcctc aaccgggccc tggagagtga catggcgcct gtcctgatca
     1081 tggccaccaa ccgtggcatc acgcgaatcc ggggcaccag ctaccagagc cctcacggca
     1141 tccccataga cctgctggac cggctgctta tcgtctccac caccccctac agcgagaaag
     1201 acacgaagca gatcctccgc atccggtgcg aggaagaaga tgtggagatg agtgaggacg
     1261 cctacacggt gctgacccgc atcgggctgg agacgtcact gcgctacgcc atccagctca
     1321 tcacagctgc cagcttggtg tgccggaaac gcaagggtac agaagtgcag gtggatgaca
     1381 tcaagcgggt ctactcactc ttcctggacg agtcccgctc cacgcagtac atgaaggagt
     1441 accaggacgc cttcctcttc aacgaactca aaggcgagac catggacacc tcctaagctt
     1501 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
     1561 aa
//
