LOCUS       sequence-30751-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Itakura E, Kishi C, Inoue K, Mizushima N
  TITLE     Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes 
            with mammalian Atg14 and UVRAG.
  JOURNAL   Mol Biol Cell. 2008 Dec . 19(12):5360-72.
  PUBMED    18843052
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Dec . 19(12):5360-72."
COMMENT     Sequence Label: pCI-neo-HA-hUVRAG
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          169..301
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     284..303
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        346..364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             388..477
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca
       61 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg
      121 actctcttaa ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      181 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      241 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      301 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      361 taggctagcc tcgagagaat gatcttttac ccatacgatg ttcctgacta tgcgggctat
      421 ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga ttacgctgct
      481 cagtgcactc gagaattggg gaattcagat ctgatcgaga tgagcgcctc cgcgtcggtc
      541 gggggccagg tcccccagcc acccccgggc ccggccgctg ctctgcctcc cggttctgcc
      601 gcgcgggccc tgcatgtgga gctgccgtct cagcagcggc gtcttcgaca tcttcggaac
      661 attgctgccc ggaacattgt taatagaaat ggccatcagc tccttgatac ctactttaca
      721 cttcacttgt gtagtactga aaagatatat aaagaatttt atagaagtga agtgattaag
      781 aattccttga atcccacgtg gcgaagtctc gattttggaa ttatgccaga
//
