LOCUS       sequence-307512-        2312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bioluminescent repair reporter (BLRR) for DNA double strand break 
            (DSB) repairs: homology-directed repair (HDR) and non-homologous end
            joining (NHEJ)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Chien JC, Tabet E, Pinkham K, da Hora CC, Chang JC, Lin S, Badr CE, 
            Lai CP
  TITLE     A multiplexed bioluminescent reporter for sensitive and non-invasive
            tracking of DNA double strand break repair dynamics in vitro and in 
            vivo.
  JOURNAL   Nucleic Acids Res. 2020 Aug 14. pii: 5892751. doi: 
            10.1093/nar/gkaa669.
  PUBMED    32797168
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 14. pii: 5892751. doi: 10.1093/nar/gkaa669."
COMMENT     Sequence Label: pLenti-BLRR
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             591..644
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc caagcccacc
       61 gagaacaacg aagacttcaa catcgtggcc gtggccagca acttcgcgac cacggatctc
      121 gatgctgacc gcgggaagtt gcccggcaag aagctgccgc tggaggtgct caaagagatg
      181 gaagccaatg cccggaaagc tggctgcacc aggggctgtc tgatctgcct gtcccacatc
      241 aagtgcacgc ccaagatgaa gaagttcatc ccaggacgct gccacaccta cgaaggcgac
      301 aaagagtccg cagatcgaat taccctgtta tccctacacc tataacatct cgcgatcgtc
      361 gacattcccg agattcctgg gttcaaggac ttggagccca tggagcagtt catcgcacag
      421 gtcgatctgt gtgtggactg cacaactggc tgcctcaaag ggcttgccaa cgtgcagtgt
      481 tccgacctgc tcaagaagtg gctgccgcaa cgctgtgcga cctttgccag caagatccag
      541 ggccaggtgg acaagatcaa gggggccggt ggcgactagc ttattccggt gagggcagag
      601 gaagtcttct aacatgcggt gacgtggagg agaatccggg ccctatgaag ataattatcc
      661 tttctgtgat tctggcttac tgtgttacag tgaattgtca ggatgcatgt ccagtagagg
      721 cggaaccgcc atcttctacc ccgaccgtac caacctcctg cgaagctaaa gaaggggagt
      781 gcatcgatac aaggtgcgct acctgcaaac gggatatcct gtccgacgga ctttgcgaaa
      841 ataaacccgg gaagacctgc tgtcgaatgt gtcagtatgt catcgaatgc cgggtcgagg
      901 ccgccggtta ttttagaaca ttttacggta aacggtttaa tttccaggaa cccggcaaat
      961 acgtactggc tcgcggcacc aagggtggcg actggagcgt caccctgaca atggaaaacc
     1021 tggacgggca gaaaggagcc gtgcttacta aaactaccct ggaggtggcg ggagacgtaa
     1081 ttgacatcac tcaggcaacg gctgacccaa taaccgtgaa cggaggagct gatcccgtga
     1141 ttgcaaaccc tttcactatt ggcgaggtca cgattgccgt cgtcgaaatt ccaggcttca
     1201 acatcacagt gatcgagttc ttcaagctga tcgtcattga tatcctcggc ggacggtccg
     1261 ttcgcatcgc acctgacaca gccaacaagg gcctgatctc tggcatttgt ggtaacttgg
     1321 aaatgaatga tgctgatgac tttacaacgg acgccgacca actggctatt caacctaata
     1381 tcaacaaaga gtttgatgga tgtccctttt acggaaatcc ttcagacatc gaatactgca
     1441 aaggcctcat ggaaccgtac cgggccgttt gcagaaataa catcaacttc tactattata
     1501 ctctgagctg cgcatttgca tactgtatgg gcggtgagga gagagccaaa catgtgcttt
     1561 tcgactatgt ggagacctgc gccgccccgg agactcgcgg tacctgcgtc ctgagcggcc
     1621 ataccttcta tgacaccttc gataaggcta ggtaccagtt ccaagggcct tgcaaagagc
     1681 tcctgatggc cgcagattgt tactggaaca cttgggacgt caaagtttcc catcgggacg
     1741 tagagagcta cacggaagtt gagaaggtga ccatcaggaa gcagagtacc gtcgtagacc
     1801 tgatcgtcga cggcaagcag gtaaaggtag gaggcgtgga cgttagtatt ccgtattctt
     1861 ctgaaaatac gagcatctac tggcaggatg gagacattct gacaaccgcc atccttccag
     1921 aagctctggt ggtgaagttt aacttcaagc agctgctggt agtgcacatt cgcgacccat
     1981 tcgacgggaa aacctgtggg atttgcggca actacaacca ggactcaact gacgatttct
     2041 ttgacgccga aggggcttgc gctcttaccc caaatccgcc tggatgcacc gaagagcaaa
     2101 agcctgaagc ggaacggctg tgcaattcac tgtttgattc ttcaatagat gagaaatgca
     2161 acgtgtgtta caaacctgac cgcatcgcac gctgcatgta tgagtattgc ctgagaggtc
     2221 aacaagggtt ctgcgatcac gcgtgggaat ttaagaaaga atgctacata aagcacgggg
     2281 atacattgga ggtgccgcca gaatgccagt ag
//
