LOCUS       sequence-307519-        3181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Tol2 transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: CMV-Tol2 transposase
FEATURES             Location/Qualifiers
     source          1..3181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1012..1030
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1012..1029
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1062..1071
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1068..3017
                     /codon_start=1
                     /product="transposase of the Tol2 transposon from the 
                     medaka fish Oryzias latipes (Kawakami, 2007)"
                     /label=Tol2 transposase
                     /translation="MEEVCDSSAAASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKM
                     KCVLCLPLNKEISAFKSSPSNLRKHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQL
                     KVDSVFPVKHVSPVTVNKAILRYIIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLR
                     SKIAEAALIMKQKVTAAMSEVEWIATTTDCWTARRKSFIGVTAHWINPGSLERHSAALA
                     CKRLMGSHTFEVLASAMNDIHSEYEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEA
                     RRCESDDTDSEGCGEGSDGVEFQDASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQ
                     KALSNEHYKKLYRSVFGKCQALWNKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAV
                     DRILQICKEAGEGALRNICTSLEVPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQ
                     LGWLLPSVHQLSLKLQRLHHSLRYCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKF
                     RTSWTNDETIIKRGMDYIRVHLEPLDHKKELANSSSDDEDFFASLKPTTHEASKELDGY
                     LACVSDTRESLLTFPAICSLSIKTNTPLPASAACERLFSTAGLLFSPKRARLDTNNFEN
                     QLLLKLNLRFYNFE"
     polyA_signal    3047..3181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3071..3090)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3125..3144
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ttggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcagctt gatttaggtg
     1021 acactataga atacaagcta cttgttcttt ttgcaggatc cgccaccatg gaagaggtgt
     1081 gcgactcttc cgccgcagct tcatctaccg tccagaacca gcctcaggac caagagcacc
     1141 cctggccata tctgagagag ttcttctccc tgagcggagt gaataaggac tcatttaaaa
     1201 tgaagtgtgt gctgtgtctt ccactcaaca aagagattag cgcgtttaaa tcctcaccct
     1261 cgaacctgag gaagcacatt gagcgtatgc accctaacta cctgaaaaat tactccaagc
     1321 tgaccgccca gaagagaaag ataggcacta gtactcacgc atccagcagt aagcagttga
     1381 aagtagactc agtgttccct gttaagcatg tgtcccctgt aacagttaac aaggctattc
     1441 tccggtacat tatccaaggt ctgcacccgt tcagcaccgt cgacctaccc tccttcaagg
     1501 agctgattag tacgcttcag cctggaatta gtgtgattac tcgccctact ctgagaagca
     1561 aaatcgcaga agcggcgctg atcatgaaac agaaagtgac tgctgcaatg agcgaggtgg
     1621 aatggatcgc gacgacaacc gactgttgga cagcacgtag gaagtccttt ataggcgtca
     1681 ctgcccactg gattaacccc ggttcactgg agcgacactc agcagccctg gcctgcaaac
     1741 gccttatggg atctcacact tttgaggtgt tagcgtctgc tatgaacgac atccactcgg
     1801 aatacgaaat tagggacaag gtggtctgta ccacaacaga ctctggatct aactttatga
     1861 aagcgtttcg cgtttttggc gtggagaaca atgatatcga aacggaggcc agacgctgtg
     1921 agtcagacga cactgacagc gaaggctgcg gcgaaggttc agacggcgtc gaatttcagg
     1981 acgcgagtcg cgttctcgac caggatgatg gatttgaatt tcagttacca aagcaccaga
     2041 agtgtgcctg tcatcttcta aacctcgtct cttcggtcga cgcacaaaaa gctctgtcca
     2101 acgagcatta caagaagctc taccggtctg tcttcggcaa gtgtcaggcc ctttggaaca
     2161 agtcctccag atccgctctc gccgcagagg cggtagagag tgagtccaga cttcagctgc
     2221 tgagacctaa tcagacacgc tggaacagca ctttcatggc agtggaccga atcttacaaa
     2281 tctgtaaaga agccggtgag ggcgctctcc gcaacatctg tacatcactg gaagtcccta
     2341 tgtttaaccc agctgagatg ctcttcttga cagagtgggc taacactatg cggccagtcg
     2401 ctaaagtcct cgacatcctt caggccgaaa ccaatacaca gttaggctgg ctccttccaa
     2461 gcgtgcacca actcagtctg aagctccaaa gactgcatca tagtctgcgg tactgcgatc
     2521 ccctcgtgga tgctctgcag cagggaatcc agactcggtt caaacacatg ttcgaagacc
     2581 ctgagattat cgctgcagcc atccttctcc caaaatttcg aacctcgtgg acaaacgatg
     2641 agaccattat taagcgcggc atggattata tccgcgtcca ccttgaaccc ctggaccaca
     2701 aaaaagagct ggctaattca agtagtgacg atgaggattt ctttgccagc ttgaagccga
     2761 cgacacacga ggcctcaaag gagctggacg gttacctggc ctgcgtcagc gatacaaggg
     2821 agtcactgtt gaccttccct gcaatctgtt cgctgtcgat caagacaaac actcccttgc
     2881 ctgctagtgc agcgtgtgag agactcttta gcacagcagg actcctgttc agccctaaga
     2941 gagcaaggct tgataccaac aactttgaga accagttgct tctgaaactg aatttacgat
     3001 tttacaactt cgagtgatct agaactatag tgagtcgtat tacgtagatc cagacatgat
     3061 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3121 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3181 t
//
