LOCUS       sequence-30753-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Itakura E, Kishi C, Inoue K, Mizushima N
  TITLE     Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes 
            with mammalian Atg14 and UVRAG.
  JOURNAL   Mol Biol Cell. 2008 Dec . 19(12):5360-72.
  PUBMED    18843052
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Dec . 19(12):5360-72."
COMMENT     Sequence Label: pCI-neo-Myc-hAtg14
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          163..295
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        340..358
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             379..408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             415..444
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             451..480
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc
      121 ttaaggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      181 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      241 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
      301 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
      361 agcctcgaga gaatgggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa
      421 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
      481 aacgctcgag aattggggaa ttccatggcg tctcccagtg ggaagggagc ccgggcgctg
      541 gaggctcctg gctgcgggcc ccggccgctc gcccgggacc tggtggactc cgtggacgat
      601 gcggaggggc tgtacgtggc tgtggagcgc tgcccgctgt gcaacactac ccgccggcgg
      661 ctgacctgcg ccaaatgcgt tcagagcggc gatttcgtct acttcgacgg ccgcgaccgg
      721 gagaggttta tcgacaagaa ggaaaggtta agccgactta agagcaagca agaagaattt
      781 cagaaagaag tgttaaaagc tatggaagga aaatggataa cagatcagtt gagatggaaa
      841 ataatgtcct gcaagatgag gattgaacag ttaaaacaaa caatatgtaa aggaaatga
//
