LOCUS       sequence-30754-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Itakura E, Kishi C, Inoue K, Mizushima N
  TITLE     Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes 
            with mammalian Atg14 and UVRAG.
  JOURNAL   Mol Biol Cell. 2008 Dec . 19(12):5360-72.
  PUBMED    18843052
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Dec . 19(12):5360-72."
COMMENT     Sequence Label: pCI-neo-HA-hAtg14
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          164..296
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     279..298
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             383..472
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gctcgtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
       61 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagtgactct
      121 cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      181 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      241 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      301 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
      361 tagcctcgag agaatgatct tttacccata cgatgttcct gactatgcgg gctatcccta
      421 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
      481 cactcgagaa ttggggaatt ccatggcgtc tcccagtggg aagggagccc gggcgctgga
      541 ggctcctggc tgcgggcccc ggccgctcgc ccgggacctg gtggactccg tggacgatgc
      601 ggaggggctg tacgtggctg tggagcgctg cccgctgtgc aacactaccc gccggcggct
      661 gacctgcgcc aaatgcgttc agagcggcga tttcgtctac ttcgacggcc gcgaccggga
      721 gaggtttatc gacaagaagg aaaggttaag ccgacttaag agcaagcaag aagaatttca
      781 gaaagaagtg ttaaaagcta tggaaggaaa atggataaca gatcagttga gatggaaaat
      841 aatgtcctgc aagatgagga ttgaacagtt aaaacaaaca atatgtaaag gaaatgaaga
      901 aatggagaaa aattctgaag ggcctt
//
