LOCUS       sequence-307558-        2500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, 
            Ralph PJ
  TITLE     Overexpression of Key Sterol Pathway Enzymes in Two Model Marine 
            Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.
  JOURNAL   Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 
            10.3390/ph13120481.
  PUBMED    33371196
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-12-21"; volume: "13(12). 
            pii: ph13120481. doi"; pages: " 10.3390/ph13120481"
COMMENT     Sequence Label: AC_pTpEF2
FEATURES             Location/Qualifiers
     source          1..2500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(962..981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1561..2352)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aagcttgcat gcctgcaagt cgactctaga ggagcggtga tcacaggcag caacgctctg
       61 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
      121 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
      181 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
      241 ccgagctcat tactcgcatc cattctcagg ctgtctcgtc tcgtctcagg tctccggaga
      301 gtgtgcaatg cagtcaattc aatagatatg taaaatgttg atgtaaggga aacaatcaag
      361 aacagatttc agatcaaact gaatgaactc ttgcactttg acacttgaca aacacatagt
      421 agtgtcccga taatcccaat tgaaaaacat agtaagcgat agactctctt tcactctgct
      481 tttctctgct ctcttttttt gacggttgac agacatactt ccacgtggcc gacgtaactc
      541 gttgagcggt ttgtcactat atgttcatgt ctcgaaaaga tgacgggaca ccaagattcc
      601 tgcttgcttc tacccgttga cgccgcacgc accaacaaag acatcaacgc ccgcctccac
      661 ccagccactt tacaaagtcc ttcttttcaa cacgctagtt agcacccccg cgattaatta
      721 aagagaaaag aacgtcaaga atgcgagacc accaggatac atagattacc acaactccga
      781 gcccttccac ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta
     1561 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     1621 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1681 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1741 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1801 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1861 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1921 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1981 tggctcgaag ataccagcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     2041 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     2101 gcggagaatc tcactctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     2161 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     2221 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     2281 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     2341 cgcttccctc atgcgaaacg atcctcatcc tgtctcttga tcagatattg atcccctgcg
     2401 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     2461 accagagggc gccccagctg gcaattccgg ttcgcttgct
//
