LOCUS       sequence-307565-        1997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1997)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046905
FEATURES             Location/Qualifiers
     source          1..1997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             360..380
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             381..410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      531..1119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1020..1039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1225..1884
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1291..1310)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1955..1989
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg agcaaaatgt
      121 ctgatgtgaa gtgtacttcc gttgtattgc tgtcagtcct gcaacagctg cgtgttgagt
      181 cgtctagcaa actatgggca cagtgcgtgc agctgcacaa cgacatcctc ttagcgaaag
      241 atacgaccga agctttcgaa aaaatggttt cgctgttgtc cgtacttctg tctatgcaag
      301 gtgccgttga cattaataaa ctgtgcgaag agatgctgga caaccgcgct actctgcaag
      361 agaacctgta ctttcaaagc gagcagaagc ttatttccga agaagatctc taaccggctt
      421 atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag
      481 gggcagaaag atgaatgact gtccacgacg ctatacccaa aagaaaaagc ttgagatcct
      541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      661 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaat tgatcgggca cgtaagaggt
     1141 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1201 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1261 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1321 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1381 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1441 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1501 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1561 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1621 tatttcccta aagggtttat tgagaatatg tttttcgttt cagccaatcc ctgggtgagt
     1681 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1741 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1801 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1861 gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
     1921 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1981 gttttttatt ggtcatc
//
