LOCUS       sequence-30757-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Muona M, Aranko AS, Raulinaitis V, Iwai H
  TITLE     Segmental isotopic labeling of multi-domain and fusion proteins by 
            protein trans-splicing in vivo and in vitro.
  JOURNAL   Nat Protoc. 2010  . 5(3):574-87.
  PUBMED    20203672
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2010  . 5(3):574-87."
COMMENT     Sequence Label: pHYRSF1-02
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             409..453
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(491..509)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      505..552
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattttgttt aactttaata aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc atatgatcaa aatagccaca cgtaaatatt
      121 taggcaaaca aaatgtctat gacattggag ttgagcgcga ccataatttt gcactcaaaa
      181 atggcttcat agcttctaat tgtttcaatg gtacccagta caaacttatc ctgaacggta
      241 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
      301 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
      361 aaacctacac cgtaacggaa taagcttgcg gccgcactcg agtctggtaa agaaaccgct
      421 gctgcgaaat ttgaacgcca gcacatggac tcgtctacta gcgcagctta attaacctag
      481 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      541 aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta
      601 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
      661 ccgacgacaa gctgacga
//
