LOCUS       sequence-307579-       11711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used 
            for visualization of cell-to-cell movement of Avr2. Avr2 can be 
            replaced with gene of interest via HiFi DNA assembly cloning (NEB) 
            ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Blekemolen, M. C., Tark-Dame, M., Takken, F. L.
  TITLE     Visualization and Quantification of Cell-to-cell Movement of 
            Proteins in Nicotiana benthamiana.
  JOURNAL   Bio-protocol 8(24): e3114.
  PUBMED    29481865
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Bio-protocol 8(24): e3114."
COMMENT     Sequence Label: Avr2-GFP pZK538
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        52..397
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             536..1243
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELYK"
     primer_bind     949..968
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1270..1522
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        1980..2324
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(2903..2905,2906..2908,2909..3622)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2903..2905,2906..2908,2909..3619)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2948..2969)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3209..3228)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3556..3577
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3881..3898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3881..3897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3890..3912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4100..4124
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5425..6054
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6483..7556
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7622..7816
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8052..8074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8160..8300
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8215..8234
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8315..8332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8486..9074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8566..8585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9161..9955)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10380..10404
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(10490..10742)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(10752..11303)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(11309..11689)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cccctactcc aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt
       61 tcaacaaagg gtaatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
      121 catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      181 aaaggctatc attcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      241 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      301 tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      361 ctctatataa ggaagttcat ttcatttgga gaggacagcc cagatcacta gtcttaagtc
      421 cggagctagc tctagagaga agatgaaggt acaggagggt ttgttcgtgg tggctgtttt
      481 ctaccttgct tatacgcagc tagtcaaggg gcaacctcgc aaggagtgcg gatccatggt
      541 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      601 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      661 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      721 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      781 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      841 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      901 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      961 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1021 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1081 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1141 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1201 cgagggccgc cactccaccg gcggcatgga ggagctgtac aagagatctc atgacgagct
     1261 gtaactgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     1321 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     1381 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     1441 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     1501 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagaatt cgatatcaag
     1561 cttgcatgcc tgcaggtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     1621 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     1681 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     1741 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     1801 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     1861 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac
     1921 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
     1981 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     2041 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     2101 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     2161 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     2221 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     2281 gacccttcct ctatataagg aagttcattt catttggaga ggacctcgag aattcaacac
     2341 aacatataca aaacaaacga atctcaagca atcaagcatt ctacttctat tgcagcaatt
     2401 taaatcattt cttttaaagc aaaagcaatt ttctgaaaat tttcaccatt tacgaacgat
     2461 agccatgcct gtggaagatg ccgattcatc tgtcggtcag ctccaaggac ggggtaaccc
     2521 atattgcgtg tttcccggcc gccgcacgtc ttctacttca tttactacga gcttcagcac
     2581 cgaacctctg ggttatgcta gaatgttaca cagagaccct ccatatgagc gggctggcaa
     2641 ttcgggactg aatcaccgca tttacgaacg aagtcgcgtt ggaggcctcc gcactgtcat
     2701 tgatgtcgcc cccccggacg gacaccaagc catcgccaac tacgagatcg aagtccgtcg
     2761 tattcctgtt gcaacaccta atgcggctgg agattgcttt cacactgcac gccttagtac
     2821 cggctctcga ggtccagcca caatcagttg ggatgccgac gctagttata cctactatct
     2881 tactatctca gaggatggat ccatggtgag caagggcgag gagctgttca ccggggtggt
     2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3601 catggacgag ctgtacaagt aagagctcgc gcgcggggat cctctagagt ccgcaaaaat
     3661 caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta
     3721 gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
     3781 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     3841 aaaaccaaaa tccagtgacc tgcaggcatg caagcttggc actggccgtc gttttacaac
     3901 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3961 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4021 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
     4081 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     4141 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     4201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     4261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     4321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     4381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     4441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     4501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     4561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     4621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     4681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     4741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     4801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     4861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     4921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     4981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     5041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     5101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     5161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     5221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     5281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     5341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     5401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     5461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     5521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     5581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     5641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     5701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     5761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     5821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     5881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     5941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     6061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     6121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     6181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     6241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     6301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     6361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     6421 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     6481 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     6541 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     6601 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     6661 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     6721 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     6781 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     6841 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     6901 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     6961 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7021 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     7081 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     7141 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     7201 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     7261 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     7321 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     7381 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     7441 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     7501 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     7561 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     7621 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     7681 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     7741 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     7801 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     7861 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     7921 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     7981 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     8041 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8101 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8161 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     8221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8281 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9121 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
     9181 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     9241 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     9301 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     9361 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     9421 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
     9481 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     9541 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     9601 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     9661 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     9721 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     9781 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     9841 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
     9901 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
     9961 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    10021 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    10081 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    10141 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    10201 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    10261 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    10321 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    10381 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    10441 acgtttttaa tgtactgaat taacgccgaa ttaattcgag ctcggatctg ataatttatt
    10501 tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata
    10561 aagccacgca catttaggat attggccgag attactgaat attgagtaag atcacggaat
    10621 ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc
    10681 catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca
    10741 agattctgca gtcaaatctc ggtgacgggc aggaccggac ggggcggtac cggcaggctg
    10801 aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc
    10861 agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc
    10921 agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg
    10981 gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtcgac
    11041 tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc gatgccggcg
    11101 acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg
    11161 tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg
    11221 tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg
    11281 gccgggcgtc gttctgggct catcgattcg atttggtgta tcgagattgg ttatgaaatt
    11341 cagatgctag tgtaatgtat tggtaatttg ggaagatata ataggaagca aggctattta
    11401 tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc acgcgcattc cgttcttgct
    11461 gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg gcgtgtcagc gtatctattc
    11521 aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat agaaacagaa tacccgcgaa
    11581 attcaggccc ggttgccatg tcctacacgc cgaaataaac gaccaaatta gtagaaaaat
    11641 aaaaactgac tcggatactt acgtcacgtc ttgcgcactg atttgaaaaa tctcagaatt
    11701 cgagctccct a
//
