LOCUS       sequence-307580-       15203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211, 
            alpha-L-Galp_697, alpha-L-Araf_996 and beta-Xylp_1710 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15203)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1276
FEATURES             Location/Qualifiers
     source          1..15203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9071..9098
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(9116..9132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9116..9132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9129..9151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9140..9156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9164..9170,9171..9188,9189..9194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9209..9230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9424..10012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9504..9523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10183..10974,10975..11043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPEMLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10806..10825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11044..11148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11216..11234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11272..11294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11394..11413
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11607..11629
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11622..11638
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             12468..12476
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(14199..14993)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
       61 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
      121 atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc
      181 tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata
      241 gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac
      301 ctcaaatggt tcgctgggtt tgagtcgtga ctaagaacgt caaagtaatt aacaatacag
      361 ctatttttct catgctttta cccctttcat aaaatttaat tttatcgtta tcataaaaaa
      421 ttatagacgt tatattgctt gccgggatat agtgctgggc attcgttggt gcaaaatgtt
      481 cggagtaagg tggatattga tttgcatatt gatctattgc attgaaatga ttagttatcc
      541 gtaaatatta attaatcata tcataaatta attatatcat aattgttttg acgaatgaag
      601 gtttttggat aaattatcaa gtaaaggaac gctaaaaatt ttggcgtaaa atatcaaaat
      661 gaccacttga attaatatgg taaagtagat ataatatttt ggtaaacatg ccttcagcaa
      721 ggttagatta gctgtttccg tataaattaa ccgtatggta aaacggcagt cagaaaaata
      781 agtcataaga ttccgttatg aaaatatact tcggtagtta ataataagag atatgaggtg
      841 agagatacaa gataagagat ataaggtacg aatgtataag atggtgcttt taggcacact
      901 aaataaaaaa caaataaacg aaaattttaa ggaggacgaa agatggcaaa gataacgatt
      961 ctggaagaca agataatttt tgaaagaaga gatgagctta cggtaataga agcttatgga
     1021 gcaaattgtc tgcgttaccg ttcaacccgc aatattaagc tgtctgatga gaactggacc
     1081 ctgctgccgc cgactacaaa agctgattgt aaaacctttg gaaatgaaag agaagcaacg
     1141 atagtaaacg gcgatatttc cgcaactata agggacggct ttggctggac cggagccagt
     1201 ataagctact ttcgcaaggg gaagcagatt cttcgatccc gtgatgaggg tgatccggtt
     1261 acccgcttta cccacctggg cggtaacaat tatcaggtga aggcgatatt tgacagtaat
     1321 ccgggcgagc atatatatgg cctgggacag gagcaggagg atcagtttga ccgcaagggc
     1381 tgcacctgca atatcatgca ttacaatacc aagtcgactt tgccggttat ttattcgtct
     1441 ctgggatatg gctttttgtg gaacaatcct gcacccggcc gttgtgagac tacctttaat
     1501 catacaatgt gggttgcgga tagtgcatat cagattgatt atcttgtata tgtcggggat
     1561 actccggctg acataatgaa aatatactgt gatctgacag gctatgcccc gaaattccct
     1621 gactgggcgg ccggcttctg gcagtcaaag ctccgttatg aaagccagga ggatttgctg
     1681 gaggtggcaa gggaatacaa gaaaagagga attcctctcg cagcgatagt aatagattat
     1741 ttccactgga cggagcaggg ggaatggaaa tttgatccca atttgtggcc tgatcccaag
     1801 gcaatgtgtg acgagcttga gtcaatggga gtaaagcccg ttgtatccat ctggcctacc
     1861 attaatccca agagtgagaa ttatcatgca atggataatg agaatatgct ggtgaggacc
     1921 gagaacgggc agtatggcat attcaacttt tacggcttac agacctttat agatcccgcc
     1981 aatcctaaaa cagggccctt tgtatggagc aaggtaaggg agaattatta taagtacgga
     2041 attaagaact tttggctgga tgaggctgag ccggaggtgc atcctcagca gtttggcaat
     2101 ctgcgttttt atataggtaa cggagcccag acggcattat tgtatccata ttattatgca
     2161 aagatatttt atgatggctt aagaagtgag ggagaagagg atattataat ccttacccgg
     2221 gcagcctatc ccggcagcca aagatttggt tccctggtat ggaacggaga catcccctcc
     2281 accttcaagg ctcttcgcca gagtgtaacc agcggccttt ccatgtcaat gtgcggaatc
     2341 ccatggtgga acagcgacat aggcggcttc cattccgcag atatagaaag cgactatttc
     2401 agggagctta tagtcagatg gttccagttc ggcctcttta gtccggttat gcgccttcac
     2461 ggggccagaa accgtacaaa aaaccaggtt gaccgccatc cgggcgtgaa ggagagaagc
     2521 ggcggagaca atgaaatctg gagcttcggt gaaagaaatt atcctattct taagaatatt
     2581 atagagctta gggagaggct gaagccttat attctcaaat atatggatat cgccagcaag
     2641 accggctcac cgattatgag gccgatgttc tatgattttt atgatgacga gatttgctac
     2701 cgacttgatg atcagtacat gttcggagag gatatactct tcgctcccat tctggagcag
     2761 ggagtgacac agcgcagtgt ctacctgccg gagggtaaat gggttaatgt aaatgataaa
     2821 aaaacctacg agggcaggca aatgataact gcaagggcag agctgcatga gtttattgcc
     2881 ttcgtaagaa gcggcagcga ggtgctggag gtatttagct aattctaaat aggaggtgta
     2941 aagtatgctg gaaatattaa gatcaaacat taagagtagg gggattatca tgaaatacta
     3001 tgtagatgct aatgctgcaa aagacggcaa tggctcactt gagaagccat ttaagcgaat
     3061 tagcgatgca gcgaagctgg cgcttcccgg cgatgaggtg ttggtggcgc ctggggttta
     3121 ccgggaatat gtagatccac ttaatgcagg cactgaggat gcccggatca gttatatcag
     3181 cactacagct cttggtgcag tgattaccgg tgccgaacag gtaaagagct ggacacatta
     3241 taaggataat gtatgggtat gcaaaatctc aaacagtatt ttcggtgatt acaatccata
     3301 tacaaccctg gtatttggag actggtattt tgcaacacca aataagcaca ccggctgcgt
     3361 attcctgaat gataaggcta tgtatgaagc agctagtttg gaggaatgca tcaagggtaa
     3421 tatttatgag tacagctggg taccggaaga ctctatatat aagtggtaca ccgaacagga
     3481 tacggaaaaa gacgagacaa tcatttatgc caattttcag ggactggacc ccaataagga
     3541 aaatgtagaa ataactgttc gtcgcagatg ctttatgcca tccaaaacag gtgtcggtta
     3601 tattacggtc agaggcttta aaatcgacaa ggcagctaca acctgggcac cgcctgcagc
     3661 ctttcaggat ggtatgatcg gtcctcactg gagcaaaggc tggattatcg aggattgcga
     3721 gatctcaaac agcaagtgcg caggcatttc tttgggtaaa tatcttgaca cggacaatga
     3781 gcattatttc acatataagc atgtaaaaag cgccacacaa atggaacgtg atgctgtctg
     3841 ccgcggccag tatcatggct ggcttaagga aaaaatcggc ggccatatag ttcgccgctg
     3901 caacatccac cattgtgagc agggaggtat tatcggccga atgggcggtg tgttcagcat
     3961 catagaggac aaccatatcc accatatcaa taatatgatg gagcttggcg gagcagaaat
     4021 ctcaggaatt aagctgcatg ctgcaattga tgtgctcatt cggcgtaacc atatccacca
     4081 ttgtacaatg ggaatatggt gtgactggga agcacagggt acccgcatta gccagaacct
     4141 gctccatgat aaccagaggc ctccgtttgc caagaatcta ccgggcagca tgatgtccca
     4201 ggatctgttt gtggaagtca gccatggacc tactcttatc gacaacaata tcctgctttc
     4261 ggatgtcagc ctccgctttg caacacaagg tgtggctatg gttcacaatc ttatctgcgg
     4321 ctcctttacc agtgtcggtg gtggaaccac atggcgctat acaccatacc atatccccca
     4381 ccgtaccgag gtcatgggct ttatgaccat tcttcatggt gacaatcgtt tctacaacaa
     4441 tatcttcatc cagaaatggc ctaatgaaga atataccacc tatgacgatc aggatgcgca
     4501 gaatgccttt agggaaaaca gacaggttgg cactcatgta tttgatgaat atccaagcta
     4561 tgatgaatgg atttcccagt tcggtttttc acattatccc aatatgagag ccctggagcc
     4621 tgttcatttt ggccatctgc caatctggag cgatggaaat gtgtatctga acggtgccaa
     4681 agcgtggaaa aaggaagcaa acagcctggt aattgaaaaa acggatcagg agcttaaggt
     4741 ggagctggtg gaaaaggacg gccagtatta tctgaacact aatatttttg attacatcaa
     4801 ggatttcaat gcccgtatga taaacacaga aatactcggc aaagcctttg agccggagca
     4861 gtactttgaa aatgcggacg gtacgccaat tcgctttgac tcagacttct tcggaaatca
     4921 tcgtggggtt catgtattgg caggcccgtt tgcatctgcc tctgaaaata taaagctata
     4981 agatagcgca tggaggataa aaaatgaaaa agcaaggctt taacccttat cttccttcct
     5041 gggaatatat ccctgatgga gaaccctatg tatttaatga cagagtctat gtatatggct
     5101 cccatgaccg tttcaatgga tacgcattct gcctgaatga ttatgtttgc tggtctgctc
     5161 ctgttgacaa cctggctgat tggcgctatg aaggtgtcat attaagagct gaggatgtag
     5221 agaacaatga tgaccgcgac agctgccttt atgctcctga tgttacggta ggtccggacg
     5281 gaagatatta cttatattat gttgacagca ggcgttcaat agtatctgtt gcagttagtg
     5341 atactcctgc aggatattat aatttttatg gatatgttca ttatcccgac ggaacacgtc
     5401 tgggtgagcg ggaaggcgac gagccacaat ttgatccggc tgttctcaca gagggtgaca
     5461 aaacctatct ctatacaggc ttctgcgcaa gaggagacaa atccagacac ggtgccatgg
     5521 caaccgtact tggtccggat atgctgacta ttatagaaga accggtattt atcgtaccca
     5581 gccagccata cagtgaaggc acaggctatg agggacatga attctttgaa gctccttcca
     5641 tcaggaagcg tggagataca tattacttta tatactcctc aattgtaatg catgagctgt
     5701 gttatgccac cagcaagcat ccgaccaagg atttcaaata cggcggtgtt atcgttagca
     5761 actgcgatct ccatatcgac agctacaagc ctgccaatat gatggcttac taccctggca
     5821 acaatcacgg aagcatagta cagattaagg acaaatggta tatcttctac cacagacata
     5881 ctaacggaac ccagtacagc agacagggct gcatggagga aatcaaattc ctcgaggacg
     5941 gctctatacc tcaggttgaa ataacctcct gcggctcaaa cggcggtcct ctggagggta
     6001 aaggcgaata tgccacttat attgcatgca atctcttcct taaggaagat cctaagcttc
     6061 ccggcaacgg cagatggcct atgttctata atccatatcc ttatattacc caggatggca
     6121 aggatggcga cgaggagttg ggttacattg ccaacatgca atattccgct acagcaggct
     6181 tcaagtattt tgactgcaag ggaattaaga agataaagat taaggttcgc ggatattgcc
     6241 gcggtgactt tgaggtaaga accaaatggg acggcccggt actaggtaca atcccggtag
     6301 tattctccaa ctgctggaag gaatacagtg ccgatatagc tattccggac ggcattaacg
     6361 ccctctactt cacctatgca ggaacaggaa gcgccagctt tgcttccttt acactggaat
     6421 aaaaattaat gtaaggagga aaagaattgg caagagataa cacatatgaa agaataagtt
     6481 ctcgaaaaat atcaggtgaa agggctacag taatggaaat agtaaggatt aagagcagat
     6541 actcatatat ggacatatcg aagcgcagag aaatgatttc cgatagaagc gttatttcca
     6601 ctgcgcctgc aactgacagg gctgactatt tttatacggg aaacgggcac tactggctgg
     6661 agagcgccgg aggagtagag aaggacacga ttattgtaac cgaggagcat ctttatgagc
     6721 ctctttggag caaaacgcct aatccgcccg atttgcgcgg atgtattgat gatatccgca
     6781 ggtgcgtcct ggagggaaat cccgaaaagg cagatgagta tattgacagg gcacagcgca
     6841 tggccggtta cgataagctc atgaacatgg acagcaaaat tgtctacccc atcggctcac
     6901 ccagagtgca caaggccttc agctttgatg tcacaagaga aggactagat acggacagtg
     6961 tatataatta tataagatgg ctcgatatgg agaccgggct tattacgaac cactttgaga
     7021 tagcgggtga aagctatgaa agcaggatag cagcatcgtt tgccgacaat ttctctgctt
     7081 ttgaatttac cgcacccaga ggcaagctta atatctctct cgaaataaaa ctgcccggcg
     7141 ggccgaatat gttcggtgtc ccgaccatgg tcggttcgtc tcacaaatgg actgttaagg
     7201 aaaacaggct tatcctcagc tggcaatata atcccgaact gacaagcaag gggtattatg
     7261 ccattatcga cgcctcatgt gaggacggag agctaagcat cagcagtgag aaaatagaaa
     7321 cgaagaattc gacccgactg tatattgccg ttaaggtcat taagatagag gataatttcg
     7381 actttgatgg atataaggtc ttcgaggctg agtttgataa gacagacaaa aggcttggca
     7441 ggcttttaca ggggaatgcg gccatcctcg gggagaagat gaaccgctca tatattactc
     7501 tgggcaagaa ggaagactgc ttactttcgg gcgaggagct ccttgagaaa tgtcacagtg
     7561 agaaggagct ttatccaatg atgatggata agctgtacga catgggaagg ttctaccaga
     7621 ttgttgatac gggcgacatt ccgcctatgt ggggacagca caacatcaat accaatctcc
     7681 aggtatgcgc aggcaacaat acaggtctct ttgacgaaat ggatgtttac ttcaggtact
     7741 atgaaagcaa gtttgatgac ttcagaacca atgccaggct tctctttaat gcgagagggc
     7801 ttctggcaag tgttcactgt gacccggatt cgggcctgct ctatcatttt tcaaagactt
     7861 atccgcatta ctgctggaca gggtgtctgg gctggatata taacgaattc tggggatact
     7921 atcttgttac aggagataag aactttctgg agaataggat tatcccggca cttaaggaaa
     7981 ttgcactgtt ctttgaggat tacgcatgcc ttaaggatga gaagggaaag agcatatttg
     8041 ttccttcgtt ctcgcctgag gatcccactc ctaatcctga ttactcaaca gttacctgca
     8101 aggacattca tcccaccaga atcaactcgg tgatggatat cgcaatatgc cgcgaggtgc
     8161 tcacaaacct cattgaaggc tgcaaaatac tcggcataga ggaaaaaaat attccaaagt
     8221 ggaaaagaca gctggaggag cttcccgtat accttaccga tgaggaaggc ggccttaagg
     8281 agtgggcatg ggagtcgata gaggagaact ataatcaccg acatgtttct catcattacg
     8341 atatatggcc aggaagagcc gtatctgtgg atagggagcc cgagctggca cgcgcaataa
     8401 agatttctaa cagaaagcgc gcccagcagg acgattccgc acacggaatc attcacgggg
     8461 ccttctgtgc gttaagactg aaggataagg aagaatttat ccagaacatg tcgcagctga
     8521 ttaatcacgg ctttgtaaga agaaaccttg caacggcaca tttcccttac agaggtcaat
     8581 tccccgatct tcagggcgca atgcctgctc ttctgattga gatgtgcgtt tactcagagc
     8641 ccggatttgt ggagttcttg ccggctcttc ccgatgattt cgcgcagggc aggcttgtcg
     8701 gggtgtggct ctataccttt gcaaagttgg aaagcatgga gtggagcgaa aaggaaatca
     8761 aggtatccat aacgtccaac agggatcaga ccatcacgtt tatgttaaga aacagagcgg
     8821 caaaattctt tgtaaatgga aaagaatatt atccctgtga cggaaaaatc gactaccact
     8881 ttagcgaggg cgaaaaagct gatatctata ttgagctgtg actgggcctt tcgttttatc
     8941 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     9001 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     9061 caaattaagc agaaggccat cctgacggat ggccttttag cttggcgtaa tcatggtcat
     9121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcagatctgc ggcgagcggt
     9301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcatttctg ggtgagcaaa
    10981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11161 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11221 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    11281 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11341 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11401 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11461 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11521 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11581 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
    11641 attgacaaag ttttctattt gtgttaacat tgtttatata atagtgaaca gtgttaagat
    11701 taaatgtgag gtgtttgtat ggatattaat gattataaag agaagggact ttatttatta
    11761 agtagtatgg atgattttat taaaattaat gatttgttta tgggtaaagt tgtttctcct
    11821 ggctatgttg cttcggtttt tggtgtttcc aggtctactg ttacacaatg gattcaaaga
    11881 cgtaaaatta gagcttttaa gtataaaggt aaggaaggtg actatatggt tatacctatt
    11941 gctgatatta ttgattacaa aagattgagt aataatgatt ttatttatga taagttagtg
    12001 aggtgattta ttttatgttt gacgatagct atgttgttaa tgagtgttcg tctaatgtta
    12061 gtgaaaatga tagagatttt tgtagtttgg ttggtcgttt tatgattatt aatggtatag
    12121 ataagttggt tattaagatt aatagaaaat ttaataggaa atctttaagt ttagatttta
    12181 gtgttgattt attcccttct atcaaagttt ctgaattagt tttttttgat gagtttaaca
    12241 aaacgtgtgg tttttatttt tcttttaatt cttttacaat ttttaaggct tttagagatg
    12301 ttcataatca taataaaata tcattttatt ttgcataatt tcgggtctgg gccgcagacc
    12361 aggcccagtg ctaacaatat taatttttaa tgttaggaat tgtttaattc ttaattgtgt
    12421 ttttaaaggt agaataatta cccattcgcc ctttagccaa caaaaattaa ggaggtataa
    12481 acatggataa aatggatttg attcttcaag atgaaagact gggtgagata tttaaagata
    12541 tagatttaac agataatgaa aagagatatc ttaaatggtt atggaaatgg gattatgaaa
    12601 cacgtgatac ttttgtatca atttttttga agctaaaaaa tggtggaaaa tgattttttt
    12661 cttatcttga tatattagaa aaaagcgtac tcacgaagta agaatttgta aaaaaagaag
    12721 gggggatttt tttggatgag agtttgtaca agcagatttt aagtaatatt attattactc
    12781 gtgattattg taaaaatgtt ttagataata taaagttcaa tgaaaaaata attgattatt
    12841 atgttatgtt acaaaatgat gtttttattg attttactaa taaaataaat tcaataaggg
    12901 attgtaataa atattggtat ttggatgttt ataaaaagca gaaaataaag gattttaaaa
    12961 agactaattt gtgtaaagat aagttctgta ataattgtaa gaaagttaaa caggcttcaa
    13021 gaatgcaaaa atatattcct gaattacaga aatacaaaga tggcttatat cattttatat
    13081 ttactgttga acatgtgcca ggtagtgaat taagagatac tattgatagg ttgtttaagt
    13141 cttttaagtc atttacaagg tatttaagtg gtaatcttaa aataaaaggt gttaattttg
    13201 ataaatgggg ttataaaggc tgtgtaaggt ctttagaggt aacttatagt atgattgata
    13261 atcatattat gtatcatcca cacttgcatg ttgcgatgat attagatcct ttttacgatg
    13321 gttttaatgt tgaaaggatg catataatta ataagtttag ttatagctat ggtgttttaa
    13381 aaaggttgtt tactgatgat gaattattaa ttcaaaaaat ttggtattta ttgtttaata
    13441 atattgaggt taacatggcc aatataaata atttagagga tggttattct tgtttagtta
    13501 ataagtttag tgattatgat tatgcggagc tgtttaagta tatttgtaaa aatactgatg
    13561 aacaaggttt acttatgact tatgatattt ttaaagattt atattttgca ttacataatg
    13621 ttcatcagat acaaggctat ggttgtttat ataatataag agatgatact caattagatt
    13681 taaaggttga tgacatttat aatgatttga ttgatttatt acaagttaca gaaaatccta
    13741 tacagtctat ggaaactgta caggatttat taaaggatac tgaatataca ataataagcc
    13801 gtaagcgtat atttaagtat ctaacacaat tatatcataa ggattgatat ttataccgtc
    13861 tgtcggactc atgcggaggg ggacttgagg gggtctcccc tcgcattgta cgacagacgg
    13921 tattattatt atacaaattt tttttatgta attttttttt gtgtaatttt tttatacaaa
    13981 taatatttca attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
    14041 cgatacaaat tcctcgtagg cgctcgggac ccctatctag cgaactttta gaaaagatat
    14101 aaaacatcag agtatggaca gttgcggatg tacttcagaa aagattagat gtctaaaaag
    14161 cttgtagtta aagcttttta gacatctaaa tctaggtgct aaaacaattc atccagtaaa
    14221 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    14281 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    14341 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    14401 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    14461 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    14521 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    14581 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    14641 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    14701 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    14761 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    14821 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    14881 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    14941 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    15001 ttccttcccc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    15061 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    15121 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
    15181 tatgatagaa tttacaagct ata
//
