LOCUS       sequence-307591-        6585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt
            deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 S 24nt-del_nostop
FEATURES             Location/Qualifiers
     source          1..6585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(125..152)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(244..330)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     379..401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     393..410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     394..410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    426..525
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4321..4420)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4437..4456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4438..4456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4461..4477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4461..4477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4908..5699
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5792..6380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6281..6300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6534..6551
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
      121 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
      181 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
      241 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      301 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
      361 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
      421 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      481 agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcatgttc gtgttcctgg
      541 tgctgctgcc actggtgagc tcccagtgcg tgaacctgac caccagaacc cagctgccac
      601 ctgcctacac caactccttc accaggggcg tgtactaccc agacaaggtg ttcagatcca
      661 gcgtgctgca ctctacccag gacctgttcc tgccattctt ctctaacgtg acctggttcc
      721 acgccatcca cgtgtctgga accaacggca ccaagagatt cgacaaccca gtgctgccat
      781 tcaacgacgg cgtgtacttc gcttctaccg agaagtctaa catcatcagg ggttggatct
      841 tcggaaccac cctggactct aagacccagt ctctgctgat cgtgaacaac gctaccaacg
      901 tggtgatcaa ggtgtgcgag ttccagttct gcaacgaccc attcctggga gtgtactacc
      961 acaagaacaa caagtcttgg atggaaagcg agttcagggt gtacagctct gctaacaact
     1021 gcaccttcga gtacgtgtct cagcctttcc tgatggacct ggaaggaaag cagggaaact
     1081 tcaagaacct gagagagttc gtgttcaaga acatcgacgg ttacttcaag atatacagca
     1141 agcacacccc aatcaacctg gtgagggacc tgccacaggg tttctctgct ctggagcccc
     1201 tggtggacct gccaatcggc atcaacatca ccaggttcca gaccctgctg gctctgcaca
     1261 ggtcttacct gaccccaggc gacagcagct ctggatggac cgccggcgcc gctgcttact
     1321 acgtgggata cctgcagcca aggaccttcc tgctgaagta caacgaaaac ggcaccatca
     1381 ccgacgccgt ggactgcgct ctggacccac tgtctgagac caagtgcacc ctgaagtctt
     1441 tcaccgtgga aaagggaatc taccagacca gcaacttccg cgtgcagccc accgagtcta
     1501 tcgtgagatt cccaaacatc accaacctgt gcccattcgg agaagtgttc aacgctacca
     1561 ggttcgccag cgtgtacgct tggaacagga agagaatcag caactgcgtg gccgactact
     1621 ctgtgctgta caactctgcc tccttctcca ccttcaagtg ctacggcgtg tccccaacca
     1681 agctgaacga cctgtgcttc accaacgtgt acgctgactc cttcgtgatc agaggagacg
     1741 aagtgagaca gatcgctccc ggtcagaccg gaaagatcgc cgactacaac tacaagctgc
     1801 cagacgactt caccggttgc gtgatcgctt ggaactctaa caacctggac tctaaggtgg
     1861 gaggcaacta caactacctg tacaggctgt tccgcaagag caacctgaag cctttcgaaa
     1921 gggacatcag caccgaaatc taccaggctg gatctacccc atgcaacgga gtggaaggat
     1981 tcaactgcta cttccctctg cagtcttacg gattccagcc aaccaacgga gtgggatacc
     2041 agccatacag agtggtggtg ctgtctttcg agctgctgca cgctcctgct accgtgtgcg
     2101 gaccaaagaa gtctaccaac ctggtgaaga acaagtgcgt gaacttcaac ttcaacggac
     2161 tgaccggaac cggagtgctg accgaatcca acaagaagtt cctgcctttc cagcagttcg
     2221 gtagagacat cgccgacacc accgacgctg tgagagaccc acagaccctg gaaatcctgg
     2281 acatcacccc ctgctctttc ggaggagtga gcgtgatcac cccaggaacc aacacctcca
     2341 accaggtggc cgtgctgtac caggacgtga actgcaccga agtgccagtg gccatccacg
     2401 ctgaccagct gaccccaacc tggagagtgt acagcaccgg atctaacgtg ttccagaccc
     2461 gtgccggctg cctgatcgga gccgagcacg tgaacaactc ctacgaatgc gacatcccaa
     2521 tcggagctgg catctgcgct tcttaccaga cccagaccaa cgcctctcag agcatcatcg
     2581 cctacaccat gagcctgggc gctgaaaaca gcgtggctta cagcaacaac tctatcgcca
     2641 tcccaaccaa cttcaccatc tctgtgacca ccgaaatcct gccagtgagc atgaccaaga
     2701 ccagcgtgga ctgcaccatg tacatctgcg gagactctac cgaatgctct aacctgctgc
     2761 tgcagtacgg cagcttctgc acccagctga accgcgctct gaccggaatc gctgtggagc
     2821 aggacaagaa cacccaggaa gtgttcgctc aggtgaagca gatatacaag accccaccta
     2881 tcaaggactt cggaggattc aacttctctc agatcctgcc cgacccatct aagccctcta
     2941 agaggtcttt catcgaagac ctgctgttca acaaggtgac cctggccgac gccggtttca
     3001 tcaagcagta cggtgactgc ctgggagaca tcgccgctag agacctgatc tgcgcccaga
     3061 agttcaacgg tctgaccgtg ctgccaccac tgctgaccga cgagatgatc gcccagtaca
     3121 cctctgccct gctggctgga accatcacct ctggttggac cttcggagct ggcgccgccc
     3181 tgcagatccc attcgctatg cagatggcct accgtttcaa cggtatcgga gtgacccaga
     3241 acgtgctgta cgaaaaccag aagctgatcg ctaaccagtt caactctgct atcggaaaga
     3301 tccaggactc cctgtcctct accgcttctg ccctgggaaa gctgcaggac gtggtgaacc
     3361 agaacgccca ggccctgaac accctggtga agcagctgtc ttctaacttc ggagccatct
     3421 cctctgtgct gaacgacatc ctgtctaggc tggacaaggt ggaagccgag gtgcagatcg
     3481 acagactgat caccggaaga ctgcagtctc tgcagaccta cgtgacccag cagctgatca
     3541 gggccgctga aatcagagct tctgccaacc tggctgccac caagatgtcc gaatgcgtgc
     3601 tgggccagtc taagagggtg gacttctgcg gaaagggata ccacctgatg tccttcccac
     3661 agtctgctcc acacggagtg gtgttcctgc acgtgaccta cgtgcccgcc caggaaaaga
     3721 acttcaccac cgccccagct atctgccacg acggaaaggc tcacttcccc agggagggag
     3781 tgttcgtgtc taacggaacc cactggttcg tgacccagag gaacttctac gagccacaga
     3841 tcatcaccac cgacaacacc ttcgtgtctg gaaactgcga cgtggtgatc ggaatcgtga
     3901 acaacaccgt gtacgaccca ctgcagccag aactggactc tttcaaggag gaactggaca
     3961 agtacttcaa gaaccacacc tctccagacg tggacctggg agacatctcc ggaatcaacg
     4021 cctctgtggt gaacatccag aaggaaatcg acagactgaa cgaggtggcc aagaacctga
     4081 acgaatctct gatcgacctg caggaactgg gaaagtacga gcagtacatc aagtggccct
     4141 ggtacatctg gctgggtttc atcgctggtc tgatcgctat cgtgatggtg accatcatgc
     4201 tgtgctgcat gacctcttgc tgctcttgcc tgaagggatg ctgctcttgc ggatcttgct
     4261 gcaagttcga cgaggacgac tccgagcctg tgctgaaggg tgtgaagctg cactacacct
     4321 acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     4381 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     4441 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     4501 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     4561 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     4621 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     4681 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     4741 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     4801 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     4861 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     4921 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     4981 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     5041 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     5101 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     5161 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     5221 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     5281 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     5341 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     5401 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     5461 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     5521 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     5581 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     5641 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     5701 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6481 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6541 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctc
//
