LOCUS       sequence-307592-       10809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing mammalian proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10809)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Bla-Ak
FEATURES             Location/Qualifiers
     source          1..10809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    294..527
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             712..756
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1139..2317
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1370..2308
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2265..2285
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2365..2482
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2590..3156
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2607..3158
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2751..2768)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2978..2997
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3161..3559
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(3604..3620)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3605..3621)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3634..4222
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3687..3707)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4105..4116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4366..4399)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4419..4599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4624..4642)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4625..4642)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4932..4951)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5165..5184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5284..5306
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5344..5362)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5430..5534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5535..5603,5604..6395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5753..5772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6566..7154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7055..7074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7308..7325
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7395..7415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7400..7729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7580..7715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8863..8928
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9058..9078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9503..9637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9540..9559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9594..9613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9805..10438
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10485..10610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
       61 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      121 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
      181 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
      241 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
      301 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
      361 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
      421 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
      481 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
      541 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
      601 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
      661 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
      721 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
      781 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
      841 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
      901 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
      961 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     1021 cgagactagc ctcgaccatc gatgtcgacg ataagctttg caaagatgga taaagtttta
     1081 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     1141 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1201 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1261 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     1321 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     1381 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     1441 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     1501 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     1561 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     1621 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     1681 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     1741 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1801 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     1861 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1921 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1981 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     2041 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     2101 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     2161 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     2221 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     2281 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgacat ttaaatttaa
     2341 ttaatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     2401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2461 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt aaacccgggc
     2521 gcgcctgcag gatccatatg cggaccgtac gtactagtgg ccacggctga tcatacgggc
     2581 tgcaggaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     2641 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     2701 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     2761 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     2821 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2881 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2941 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3001 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     3061 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     3121 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggccaagc ctttgtctca
     3181 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     3241 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     3301 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     3361 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     3421 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     3481 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     3541 ttatgtgtgg gagggctaac tagctagtcg agctcaactt cgaattcgat atcaagctta
     3601 tcgcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     3661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3781 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3841 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3901 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3961 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4021 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4081 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4141 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4201 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac
     4261 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4321 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     4381 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     4621 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     4681 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     4741 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     4801 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     4861 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     4921 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     4981 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     5041 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     5101 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     5161 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5341 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5401 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7081 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7201 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7261 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7321 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7381 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7501 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     7741 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     7801 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     7861 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     7921 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     7981 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     8041 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     8101 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     8161 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     8221 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     8281 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     8341 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     8401 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     8461 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     8521 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     8581 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     8641 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     8701 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     8761 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     8821 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     8881 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     8941 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     9001 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     9061 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     9121 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     9181 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     9241 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     9301 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     9361 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     9421 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9481 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9601 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9661 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9721 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9781 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     9841 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     9901 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     9961 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    10021 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
    10081 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
    10141 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
    10201 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
    10261 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10321 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10381 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10441 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    10501 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    10561 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    10621 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    10681 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    10741 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    10801 agacaggat
//
