LOCUS       sequence-307634-        8297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in
            place of bxb1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Hollerer S, Papaxanthos L, Gumpinger AC, Fischer K, Beisel C, 
            Borgwardt K, Benenson Y, Jeschek M
  TITLE     Large-scale DNA-based phenotypic recording and deep learning enable 
            highly accurate sequence-function mapping.
  JOURNAL   Nat Commun. 2020 Jul 15;11(1):3551. doi: 10.1038/s41467-020-17222-4.
  PUBMED    32669542
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-15"; volume: "11(1):3551. doi"; pages: " 
            10.1038/s41467-020-17222-4"
COMMENT     Sequence Label: pASPIre2
FEATURES             Location/Qualifiers
     source          1..8297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(15..863)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMMPSQWRHLNSQK
                     D"
     CDS             complement(937..1773)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     protein_bind    1928..1977
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     RBS             1996..2018
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2026..2733
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MHSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2171..2189)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2439..2458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    2792..2839
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     promoter        complement(2879..2913)
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     promoter        2955..3073
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             4653..5363
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4653..5363
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4680..4708)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5303..5323
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             5370..5402
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AANDENYALAA"
     primer_bind     complement(5431..5453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      5468..5554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             5734..5925
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5919..5941)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6027..6167
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6082..6101
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6182..6199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6353..6941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6433..6452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            6977..7085
                     /note="incP origin of transfer"
     CDS             complement(7252..8067)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8059..8078
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(8199..8293)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagtaagc ttaattaatc tttctgcgaa ttgagatgac gccactggct gggcatcatc
       61 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
      121 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
      181 acctgtcgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
      241 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
      301 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
      361 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
      421 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
      481 tgccgcgcct gcgccatccc catgctacct aagcgccagt gtggttgccc tgcgctggcg
      541 ttaaatcccg gaatcgcccc ctgccagtca agattcagct tcagacgctc cgggcaataa
      601 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
      661 atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcaggcca
      721 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcagc aaagacatct
      781 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
      841 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
      901 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
      961 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     1021 actgtcgctg aatccacagc gataggcgat gtcagtaacg ctggcctcgc tgtggcgtag
     1081 cagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     1141 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     1201 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     1261 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     1321 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     1381 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     1441 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     1501 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     1561 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     1621 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     1681 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga
     1741 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     1801 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     1861 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatct
     1921 gcagtaatcg gccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccgggccca
     1981 tgggatatct ataattttgt ttaactttaa gaaggagata tgcacatgca tagcaagggc
     2041 gaagaagata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2101 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2161 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2221 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2281 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2341 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac
     2401 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagaca
     2461 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2521 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2581 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2641 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2701 cactccaccg gcggcatgga cgagctgtac aagaggcctt aagcaaaaaa ccccgcttcg
     2761 gcggggtttt ttcgctgcga tatctaataa gggtttgtac cgtacaccac tgagaccgcg
     2821 gtggttgacc agacaaacca cgaaggttct gttaagtaac tgaacccaat gtcgttaggc
     2881 tagcacaata cctaggactg agctagccgt caatgacgct tacctcttaa gaggtcactg
     2941 acctaacagg atcccaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
     3001 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
     3061 tagactggtc gtaatgaagg gtaccataaa tgcttcaata atattgaaaa ggattctaga
     3121 tgcgagctct ggttgttatt cgtctgagcc gtgttaccga tgcaaccacc agtccggaac
     3181 gtcagctgga aagctgtcag cagctgtgtg cacagcgtgg ttgggatgtt gttggtgttg
     3241 cagaggatct ggatgttagc ggtgcagttg atccgtttga tcgtaaacgt cgtccgaatc
     3301 tggcacgttg gctggcattt gaagaacagc cgtttgatgt tattgttgcc tatcgtgttg
     3361 atcgtctgac ccgtagcatt cgtcatctgc aacagctggt tcattgggca gaagatcata
     3421 aaaaactggt tgtgagcgca accgaagcac attttgatac caccaccccg tttgcagcag
     3481 ttgttattgc actgatgggc accgttgcac agatggaact ggaagcaatt aaagaacgta
     3541 atcgtagcgc agcccatttt aacattcgtg caggtaaata tcgtggtagc ctgcctccgt
     3601 ggggttatct gccgacgcgt gtagatggtg aatggcgtct ggttcctgac ccggttcagc
     3661 gtgaacgtat tctggaagta tatcatcgtg tggtggataa tcatgaaccg ctgcatctgg
     3721 ttgcacatga tctgaatcgt cgtggtgttc tgagtcccaa agattatttt gctcagctgc
     3781 aaggtcgtga accgcagggt cgtgaatggt ctgcaaccgc actgaaacgt agcatgatta
     3841 gcgaagcaat gctgggttat gcaaccctga atggtaaaac cgttcgtgat gatgatggtg
     3901 caccgctggt tcgtgcagaa ccgattctga cacgtgaaca gctggaagca ctgcgtgccg
     3961 aactggttaa aaccagccgt gcaaaaccgg cagttagcac cccgagcctg ctgctgcgtg
     4021 ttctgttttg tgcagtttgt ggtgaaccgg catacaaatt tgccggtggt ggtcgtaaac
     4081 atccgcgtta tcgttgtcgt agcatgggtt ttccgaaaca ttgtggtaat ggtacagttg
     4141 caatggcaga atgggatgca ttttgcgaag aacaggttct ggatctgctg ggtgatgccg
     4201 aacgtctgga aaaagtttgg gttgcaggta gcgatagcgc agttgaactg gccgaagtta
     4261 atgcggaact ggtcgatctc accagtctga ttggaagtcc cgcatatcgt gcgggtagtc
     4321 ctcagcgtga agcactggat gcacgtattg cagcactggc agcacgtcaa gaagaactgg
     4381 aaggtctgga agcacgtccg agcggttggg aatggcgtga aacaggtcag cgttttggtg
     4441 attggtggcg tgagcaggat accgcagcaa aaaatacctg gctgcgtagt atgaatgttc
     4501 gcctgacctt tgatgttcgc ggtggcctga cccgcaccat tgattttggc gatctgcaag
     4561 aatatgaaca gcatctgcgt ctgggtagcg ttgttgaacg tctgcatacc ggcatgagca
     4621 ccggcggtgg cagcggcggt tctggtggct ctagcaaagg agaagaactt ttcactggag
     4681 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtccgtg
     4741 gagagggtga aggtgatgct acaaacggaa aactcaccct taaatttatt tgcactactg
     4801 gaaaactacc tgttccgtgg ccaacacttg tcactactct gacctatggt gttcaatgct
     4861 tttcccgtta tccggatcac atgaaacggc atgacttttt caagagtgcc atgcccgaag
     4921 gttatgtaca ggaacgcact atatctttca aagatgacgg gacctacaag acgcgtgctg
     4981 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaagggt attgatttta
     5041 aagaagatgg aaacattctt ggacacaaac tcgagtacaa ctttaactca cacaatgtat
     5101 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg
     5161 ttgaagatgg ttccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     5221 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgtcctt tcgaaagatc
     5281 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     5341 atggcatgga tgagctctac aaaaggcctg ctgctaacga cgaaaactac gctctggctg
     5401 cttaatgagc ggccgcggcc taggcggcct cctgtgtgaa attgttatcc gctttaatta
     5461 aaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     5521 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacag ctgggcgcgc
     5581 ccccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
     5641 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
     5701 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
     5761 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
     5821 gacgcggatg aacaggcaga catctgtgaa tcgctgcacg accacgctga tgagctttac
     5881 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     5941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6001 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6061 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6121 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     6181 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6241 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6781 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6961 ctacggggcc ggcctacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     7021 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     7081 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
     7141 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
     7201 agggttatgc agcggaaaag gacaacgcgc ggaccgcggt ccaattaatt attagaaaaa
     7261 ttcatccagc atcagatgaa attgcagttt gttcatatcc ggattatcaa tgccatattt
     7321 ctgaaacaga cgtttttgca ggctcgggct aaattcgccc aggcagttcc acagaatggc
     7381 cagatcctga taacgatccg caatgcccac acggcccaca tcaatgcagc caatcagttt
     7441 gccttcatcg aaaatcaggt tatccaggct aaaatcgccg tgggtcacca cgctatccgg
     7501 gctaaacggc agcagtttat gcatttcttt ccacacctgt tccaccggcc agccgttacg
     7561 ttcatcatca aaatcgctcg catccaccag gccgttgttc atacggctct gcgcctgggc
     7621 cagacgaaac acacgatcgc tgttaaacgg gcagttgcac accggaatgc tatgcagacg
     7681 acgcagaaac acggccagcg catccacaat gttttcgccg ctatccggat attcttccag
     7741 cacctgaaac gcggttttgc ccggaatcgc ggtggtcagc agccacgcat catccggggt
     7801 gcgaataaaa tgtttaatgg tcggcagcgg cataaattcg gtcagccagt tcagacgcac
     7861 catttcatcg gtcacatcgt tcgccacgct gcctttgcca tgtttcagaa acagttccgg
     7921 cgcatccggt ttgccataca gacgataaat ggtcgcgccg ctctgaccca cgttatcacg
     7981 cgcccattta tagccataca gatccgcatc catgttgctg ttcagacgcg gacggctaca
     8041 gctcgtttca cgctgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     8101 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
     8161 agacacaaat ttaaatcgta attattgggg acccctggat tctcaccaat aaaaaacgcc
     8221 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     8281 atcaacagga gtccaag
//
