LOCUS       sequence-307652-        8939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Gateway-compatible expression vector backbone with an 
            N-terminal Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8939)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-Firefly-ccdB
FEATURES             Location/Qualifiers
     source          1..8939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      164..299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     226..245
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             362..1387
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             362..1387
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1517..1638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1554..1573)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1608..1627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1687..1703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1687..1703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1700..1722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1711..1727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1735..1741,1742..1759,1760..1765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1780..1801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1918..1935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2089..2677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2169..2188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2848..3639,3640..3708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3471..3490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3709..3813)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3888..3907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4079..4458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4459..4662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4613..4633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4659..4683
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4749..4758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4755..6404
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4821..4839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    6450..6574
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6599..6604,6605..6622,6623..6629)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6683..7342
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6749..6768)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7684..7989
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7868..7887
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8030..8154)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8205..8222)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8211..8435
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8481..8909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8568..8587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8778..8799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8918..8938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8923..8939,1..313)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
       61 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      121 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
      181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
      241 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      301 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
      361 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
      421 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
      481 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
      541 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
      601 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
      661 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
      721 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
      781 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
      841 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
      901 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
      961 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     1021 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     1081 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     1141 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     1201 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     1261 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     1321 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     1381 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1441 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1501 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1621 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1681 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3841 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3901 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3961 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     4021 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     4081 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     4681 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagagg
     4741 atccagccgc caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     4801 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     4861 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     4921 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     4981 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     5041 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     5101 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     5161 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     5221 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     5281 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     5341 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     5401 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     5461 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     5521 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     5581 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     5641 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     5701 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     5761 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     5821 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     5881 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     5941 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     6001 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     6061 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     6121 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     6181 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     6241 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     6301 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     6361 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgagtggc ggccgctcga
     6421 gtggctcggg ctcgacctcg ggctcgggca caagtttgta caaaaaagct gaacgagaaa
     6481 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     6541 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     6601 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     6661 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     6721 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     6781 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     6841 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     6901 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     6961 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     7021 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     7081 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     7141 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     7201 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     7261 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     7321 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     7381 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     7441 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     7501 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     7561 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     7621 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     7681 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     7741 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     7801 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     7861 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     7921 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     7981 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     8041 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     8101 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgctct
     8161 atagtaactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     8221 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     8281 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     8341 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     8401 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     8461 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8761 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8821 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8881 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtg
//
