LOCUS       sequence-307659-       13399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU70.2-2.2
FEATURES             Location/Qualifiers
     source          1..13399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1565..1864
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4059..4279
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4280..5083
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9025..9045)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9025..9043)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9382..9970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9462..9481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10141..10932,10933..11001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10764..10783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11002..11106)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11133..12475
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12096..12143)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12516..12534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12876..12892)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12876..12892)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aatggattct gctgcagatc ttcgagggcg aggtagctgg agctatagcg cagccgaata
       61 tcctgtggtc gctgtgacgg cctgctctgt tctcctaatt ctatgggcga tcgctcatat
      121 cctttataac attttccttc atccgctcag aaatcaccct ggaccaaagc tctgggccgc
      181 aactcgcgta ccatggtgtt ggtatcagta ccaaggcatc ctacacgagc gtttattgga
      241 gcttcatgtc aagtacggtc acactgtccg tatcgctcct tacgaactct ccttcacctc
      301 agacacggca tggaagacga tatatggaca cagatccgtg gagatggaca aggatcctgt
      361 ttttcgcctt cacactccta ctggagcaca gaatatcctg gtggccgacc gccagacgca
      421 tattcgccaa agacgactgc tttcccatgc attttctgaa aaagcactta gggagcaaga
      481 aggtatttta caaacatatg tgagcaagct gttggaacag ctatctttga gatatactgc
      541 aggaccgctg aacatggtcg catggtacaa tttcacaacc ttcgacctga taggagatct
      601 gtcgtctggc gaaaagttcg ggtgccttga caagggtgat tacgtcccct tcgtccgcgc
      661 aacggagagg atagcaaaag agctcacctt cacccaaatg ctaatctact tcggcgtggc
      721 gggcgtccgg caattcttct tgcccaaagc tgtggccggc cagcgcgctc agaacatgaa
      781 acgcgcaatc gacaccgtgg agcgacgaat ccaacgggac actgaccgaa aagacttcct
      841 ctactatatc ctcgccgcca acgacgagaa aggcatgagc agggcagaga tccatgtaaa
      901 ttcattttcc ttcaacatcg ccggctcaga atcaagtgcg actgctcttt ccggaataac
      961 ataccacatc ctcacccgct catccgccta ctcagaactc atccacgaaa ttcgcacctc
     1021 tttcacgtcc gaagaagaga tcacaatcgc cagcaccaac gacctcaaat acctcgaagc
     1081 cgtcatttcc gaatctctcc gtctctaccc gcccgtcgcc ggcaccctcc cccgtgtcgt
     1141 gcccgcccca ggcgagacga ttgatggcat ccatgtaccc accggcacca ctgtcggtgt
     1201 ccaccacttc tcttgcttcc gccacccggc taatttccac cgcccagacg agtttctccc
     1261 cgagcgctgg cttccggaat gcaaagatgt tccgccgttt gacaaggaca acagggcggc
     1321 ggtgcagccg ttctcattcg gtccacggaa ttgtttggga aagaatctag cacgagcgga
     1381 gatgaggctg ataatggcga agctgctgtg gaggtttgat ttagagttgg ttccgggtgg
     1441 gggagggagc gggaagggaa tgggaatggg aaatggcaag agttggaggg atggacagaa
     1501 ggtctggggt ttttgggtca agccaccatt gatgtgttgg ctgaggcctg tgagtagaga
     1561 ttgagcggat ctcttatgtc tttacgattt atagttttca ttatcaagta tgcctatatt
     1621 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta
     1681 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt
     1741 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc
     1801 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc
     1861 tcattttcct tcgcatgcca gcattgaaat gatcgaagtt caatgatgaa acggtaattc
     1921 ttctgtcatt tactcatctc atctcatcaa gttatataat tctatacgga tgtaattttt
     1981 cacttttcgt cttgacgtcc accctataat ttcaattatt gaaccctcac gatccagttc
     2041 tccagtgaca cagcctttat ctggtcaaac ctttctttct aatcacctat gctgatgctt
     2101 aattaaggga tttttgtctc catcaacggc atgcgcccaa aaatgacgtt ttttttaacc
     2161 catagacacg aaactaccca ttttccaccg gcctgaccta ccaccggaac aacggccatc
     2221 tccaacttgc aagttgggga aattaagagc atcgcaggtt taatggaaga aaaaaaaaag
     2281 gtacagcaca gcgcaaatgg agttagttcc cttatgtcac acactcacac acagtcggtc
     2341 agatcaagca tactgggtgc gtataaatag agtggccatt gccaccctgt ttatctcaaa
     2401 atctgtcttg ttagtggtct tctccctttt tcaggttaca attctcttgt ttctacttag
     2461 tatataagta tatcaagcta tattaagcat actatcaact gtcaactcta tcctcaaaat
     2521 acaatacaaa atggccattc actggtccct cgaccatctc gagaagctcc tccccaagcg
     2581 actcttcgct aagctctcag agatttcttg caatcccaac gttcccatag aagctggtgg
     2641 taattaccca atcatcaatg ccgagaccgg ggatctgctt gccggcgtgc cgtacgccag
     2701 gggattccgc gttcccagga gcaaaatgcg cgctttgtgt gctgaaggaa ttgatgtcca
     2761 gtacggcaag aaccttacgg atcttgcgtt cagcgagaag ggtgaccttg tgttcgcctc
     2821 gtttagcgac ggcacgatcg cccaaggctc tgtacttctt ggcgccgatg gcccacgctc
     2881 gaaagttcga gaattcgcca tgtctggagc agaggcagcg caagtcagca agtttccgat
     2941 ctgtcatcac aatatgactg tttgctatcg agatgtggat aaggcaaagt atctgagaca
     3001 acggtttcca acgagctatt tggctctgag ccagaagtcg caccacgctt ttcagtcgat
     3061 ctctagcatg cctgatggtc aagaccatcc agaaacatgg ttgtttcatc tcgccatggc
     3121 ctggctgggt gacccggagc actcactcag ctacgaagag cgtcttgcca tcattaagga
     3181 acgtgcacaa ggtatgggcg agcctgctcg atcagcattc acgtggatcc ccgaggacac
     3241 cctggtccac aaggcagaca tcagctattg gatcacgcaa ccgtgggaca accgtggagg
     3301 tagagtcacc cttgtaggag atgctgcgca tcccatgcca ccctatcgtg gtcaaggcct
     3361 caaccactgc atctgtgaca gttctcacct cctcgatggt cttgagctcg tacgtgccaa
     3421 caaggcaacc atcaaggatg ccattactgc ctacgatgaa gaactcgtcc ctcgcggacg
     3481 tgacgaggtc aaatgctcgt tggagaacgg cctcatgctg catgactggc agaaggtgaa
     3541 ggagagcccc gtattcagga acggattcag gcctatgact ggtcacgacg gcaaggaggc
     3601 tcttagtgag catgcgcagg cgcagatgaa gagggatgag gaggagcaga gggatatccc
     3661 tgtcgctgcg acttgaacaa atcgctctta aatatatacc taaagaacat taaagctata
     3721 ttataagcaa agatacgtaa attttgctta tattattata cacatatcat atttctatat
     3781 ttttaagatt tggttatata atgtacgtaa tgcaaaggaa ataaatttta tacattattg
     3841 aacagcgtcc aagtaactac attatgtgca ctaatagttt agcgtcgtga agactttatt
     3901 gtgtcgcgaa aagtaaaaat tttaaaaatt agagcacctt gaacttgcga aaaaggttct
     3961 catcaactgt ttaaaaggag gatatcaggt cctatttctg acaaacaata tacaaattta
     4021 gtttcaaaga tgaatcagtg cgcgaaggac ataactcatt caattcaatt catcattttt
     4081 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
     4141 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     4201 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     4261 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     4321 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
     4381 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
     4441 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
     4501 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
     4561 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
     4621 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
     4681 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
     4741 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
     4801 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
     4861 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
     4921 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
     4981 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
     5041 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
     5101 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
     5161 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggataggaa
     5221 aaaaccgagc ttcctttcat ccggcgcggc tgtgttctac atatcactga agctccgggt
     5281 attttaagtt atacaaggga aagatgccgg ctagactagc aagttttagg ctgcttaaca
     5341 ttatggatag gcggataaag ggcccaaaca ggattgtaaa gcttagacgc ttctggttgg
     5401 acaatggtac gtttgtgtat taagtaaggc ttggctgggg atagcaacat tgggcagagt
     5461 atagaagacc acaaaaaaaa ggtatataag ggcagagaag tctttgtaat gtgtgtaact
     5521 tctcttccat gtgtaatcag tatttctact tacttcttaa atatacagaa gtaagacaga
     5581 taaccaacag cctttcccag atatacatat atatctttat ttcagcttaa acaataatta
     5641 tatttgttta actcaaaaat aaaaaaaaaa aaccaaactc acgcaactaa ttattccata
     5701 ataaaataac aacatggctc caagagtcgt cttgatcacg ggtgccaaca ctggcattgg
     5761 ttttcagatt gtgaggtcac tctgcagctc tgatcaagct tacgacatca tcgttggtgg
     5821 gcgatcgctg tccaaggctg aggatgccat cagctccgct gttaaggagt tcccctcctc
     5881 tcgcagtaag ctttttccaa ttcaggtcga cattgagcag gatgactcta tcaaacacgc
     5941 gttcaacgag gtgcagtcta agttcggaaa gcttgatgct ttggtaaaca atgctggcgc
     6001 gcagctcgaa gcgcagtttg ccgctggtaa gctgacggag cgcgacattt ggaaccagtc
     6061 atggaatatc aacaccgtgg gggcgcaagt aatgacctca agcttcatac ctctcctgct
     6121 ccagactcct gacccgcgcc tgctgttcat cacgtccaac gcgtccactc tcgctggaac
     6181 cgagaacctt gctctaccca ttgaccgctc tcctcctaaa gggtggccga aacgcggttt
     6241 cagtattgcg gcataccgca gtgccaaaag cggcatgaac atgatgatgc gtgaatggca
     6301 ccgcacgctg aaggaagacg gtgtgaaagt atggtgtctt gctccaggtt tctcggctac
     6361 gggccttggt gggaatccgg aggcctcaaa gaaaatggga gctggtgatc cggcggtggc
     6421 gggccctttc atcaggagtg ttttggaggg tgaaagggat ggtgacgttg gtaaggtcat
     6481 tacgaaggat ggtgtgcagg cttggtgaag tgcttttaac taagaattat tagtcttttc
     6541 tgcttatttt ttcatcatag tttagaacac tttatattaa cgaatagttt atgaatctat
     6601 ttaggtttaa aaattgatac agttttataa gttacttttt caaagactcg tgctgtctat
     6661 tgcataatgc actggaagga gaaaaaaaag gtgcacacgc gtggcttttt cttgaatttg
     6721 cagtttgaaa aataactaca tggatgataa gaaaacatgg agtacagtca ctttgagaac
     6781 cttcaatcag ctggtaacgt cttcgttaat tggatactca aaaaagatgg atagcatgaa
     6841 tcacaagatg gaaggaaatg cgggccacga ccacagtgat atgcatatgg gagatggaga
     6901 tgatacctcc attgggccga tgaagttagt cgacggatag aagcggttgt cccctttccc
     6961 ggcgagccgg cagtcgggcc gaggttcgga taaattttgt attgtgtttt gattctgtca
     7021 tgagtattac ttatgttctc tttaggtaac cccaggttaa tcaatcacag tttcataccg
     7081 gctagtattc aaattatgac ttttcttctg cagtgtcagc cttacgacga ttatctatga
     7141 gctttgaata tagtttgccg tgattcgtat ctttaattgg ataataaaat gcgaaggatc
     7201 gatgaccctt attattattt ttctacactg gctaccgatt taactcatct tcttgaaagt
     7261 atataagtaa cagtaaaata taccgtactt ctgctaatgt tatttgtccc ttatttttct
     7321 tttcttgtct tatgctatag tacctaagaa taacgactat tgttttgaac taaacaaagt
     7381 agtaaaagca cataaaagaa ttaagaaaat gactacattt gtagaatcat ccatctcgcc
     7441 gcttttcggg ctgtggcagc ctcttcccca cttgaaacat ctctattatg gttcgtcttg
     7501 cgttcaaaag catctattcg cgactctccc ttcacacgat tcgaaagttt tcatcgtcac
     7561 aggaaattct atcgcgacca agactcctct tcttcaacaa gtcgagactt tactcggaga
     7621 tcaccatgcg ggaacattca ccaaaatcag acaacatgcg ccaatggccg atattgacca
     7681 agccacggag atcatcgcca aaaatccgtt ggttgatact atcttatcgt tgggaggtgg
     7741 atctcccatc gattccgcaa aaacaatatc attccgcatg catgagagaa atggcaaatt
     7801 cctgacacat ctcacgatcc ctacgacgct gagtgctgct gagtgcacga taggcggcgg
     7861 ctacacgaaa gcagacggtg tcaagacttc agtaatgacg cccgaaattg gtgtgactgc
     7921 agtattttac gacccgggct atgccaaata tactccgttg aagctatggc tatcgactgg
     7981 tatgcgtgct atggaccacg ccgtggaatc acaatatcag ccatacgcgt cagagatgcc
     8041 atggagggca atgtcgtctt gggcagtgtc caccttcttt gaatgtctac caatagccaa
     8101 ggcatctcat cccagcgacg aagacgttat aacgagatta ctacttgctg cgtttgccag
     8161 cttgggcttc cgcgatggag agactagctg cctcactctc ggtcccgtgg tccagcttaa
     8221 ggcgcgggcc aagcaagagg acgcaaaaca aattgcaaga ctcctgcctt ggattggcgg
     8281 gacgtcgagc ggtgatgatc tcaaagatgc tttggaggtc ggatgcaaga tcagtgcgtt
     8341 agtagagagc ctgggattac atcaaagcct tacagagagg ggtattggca gagaccagat
     8401 tccaatcgtg gtggaacggg ctactggtgg tttgaaagaa ggctcactat atgacatcgt
     8461 cacgcttttg gtcgagggtt tatactaagg agattgataa gacttttcta gttgcatatc
     8521 ttttatattt aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct
     8581 ggttattcta aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt
     8641 aacaggcgac agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat
     8701 tttgtcaatg gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct
     8761 ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg
     8821 tgttcccaat tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat
     8881 tttaaagtat atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa
     8941 gccatcgtta ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc
     9001 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact
     9061 caaacaagat atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac
     9121 tatcacgtgg gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa
     9181 aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca
     9241 gggcttgatc tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc
     9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11101 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
    11161 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
    11221 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
    11281 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
    11341 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
    11401 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
    11461 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
    11521 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
    11581 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
    11641 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
    11701 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
    11761 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
    11821 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
    11881 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
    11941 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
    12001 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
    12061 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
    12121 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
    12181 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
    12241 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
    12301 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
    12361 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
    12421 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact
    12481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    12541 gtctcgcgcg tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg
    12601 cgcgccatta ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg
    12661 aatcctgttt ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca
    12721 gggtgcactt tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta
    12781 agcgcgggta ttgaaccagg cttatgccca agatcgtagc aagcatttaa atgacggtat
    12841 cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta gattaattaa
    12901 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
    12961 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
    13021 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
    13081 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
    13141 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
    13201 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
    13261 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
    13321 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
    13381 taactatatc gtaatacac
//
