LOCUS       sequence-307684-       12339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for 3xFLAG-tagged NIPBL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12339)
  TITLE     Suske Lab plasmids
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pN3-Nipbl
FEATURES             Location/Qualifiers
     source          1..12339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        273..576
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        577..780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     731..751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             835..900
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    9339..9460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9376..9395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9430..9449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9467..9922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9604..9625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9816..9835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9949..10053
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10055..10412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10055..10246
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10078..10098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10263..10398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10447..11241
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10447..11241
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10501..10520)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11111..11130
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11429..11448)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11473..11520
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(11849..12339,1..98)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(12338..12339,1..18)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
       61 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      181 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
      241 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      421 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      781 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctccac catggactac
      841 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
      901 aagcttcgaa ttctgcagtc gacggtaccg cgggccctga atggggatat gccccatgtc
      961 cccataacta cgcttgcggg gattgctagt cttacggacc tcctgaacca gctgcctctc
     1021 ccatctcctt tacctgctac aactacaaag agcctcctct ttaattcacg aatagcagaa
     1081 gaggtgaact gccttttggc ctgtagggat gacaatttgg tttcacagct tgtccatagt
     1141 ctcaaccagg tatcaacaga tcacatagaa ctgaaagata accttggcag tgatgatccc
     1201 gaaggcgata tacccgtctt gttgcaggcc gtcctggcaa ggagtccgaa cgttttcagg
     1261 gagaaaagca tgcagaacag atatgtacag agtggaatga tgatgtctca gtataaactt
     1321 tctcagaatt ccatgcacag tagtcctgca tcttccaatt accaacaaac cactatctca
     1381 catagcccct ccagccggtt tgtgccacca cagacaagct ctgggaacag atttatgcca
     1441 caacaaaata gcccagtgcc tagtccatat gccccacaga gtcctgcagg atacatgcca
     1501 tattcccatc cttcaagtta cacaacacat ccgcagatgc agcaagcatc ggtatcaagt
     1561 cctattgttg ccggtggctt gagaaacatc catgataaca aagtttctgg tccattatct
     1621 ggcaactcag ctaaccatca tgctgataac cctagacatg gctcaagtga cgactaccta
     1681 cacatggtgc acaggctaag tagtgatgat ggcgattctt caacaatgag gaatgctgca
     1741 tctttcccct taagatctcc tcaaccagtg tgttctcctg ctggaagtga cggaacacct
     1801 aaaggctcaa gaccaccttt aattctacag tctcagtctt taccttgttc atcacctcga
     1861 gatgttcctc cagatatctt actagattct ccagaaagaa aacaaaaaaa gcaaaagaaa
     1921 attaaattag gcaaggatga aaaagaccag aacgagaaag ctgcaatgta tgatataatt
     1981 agctctccaa ccaaggactc cactaaactt acattaagac tttctcgtgt aagatcttca
     2041 gatatggacc agcaagacga tatgctatct ggtatggaaa atagcaatgt ttcagaaaat
     2101 gatattcctt ttaatgtgca gtatccagga cagacttcaa aaacacccat tactccacag
     2161 gacgtaaacc gcccacttaa tgctgctcag tgtttgtcgc agcaagaaca aacagcattc
     2221 cttccagcaa atcaagtgcc tgttttacaa cagaacactt cagttgctac aaaacaacca
     2281 cagacttctg tagtacagaa tcagcagcag gtatcacaac aggggccgat atatgatgaa
     2341 gtcgaattgg acgcgttggc agaaattgaa cggatagagc gagaatcagc cattgaaagg
     2401 gagcgcttct caaaggaagt tcaagataag gataagcctt tgaaaaaaag aaaacaagat
     2461 tcttacccac aggaggctgg gggtgctaca ggaggtaata gaccagcttc tcaggagacg
     2521 ggttctacgg gaaatgggtc aaggccagca ttaatggtta gcattgatct tcatcaggca
     2581 ggaagagtgg actctcaggc ttctataact caggattcag actccataaa aaagcctgaa
     2641 gaaaccaaac agtgtaatga tgcgcccatt tctgttcttc aggaagatat tgttggaagt
     2701 cttaagtcta taccagaaaa ccatcctgag actcctaaaa aaaagtctga tcctgagctt
     2761 tcaaaaagcg aaatgaaaca aaatgaaagt aggttgtcag aatctaaacc aaatgaaaat
     2821 cagctggggg agtcaaaatc aaatgaaagt aagttagaaa ctaaaactga gaccccaaca
     2881 gaagaactta agcagaacga gaacaaaacg acagagtcca aacagagcga gagcgctgta
     2941 gttgagccta aacagaatga aaatagaccg tgtgacacaa aaccaaatga caacaaacag
     3001 aacaacacca ggtcagaaaa cacaaaagca agacctgaaa ccccaaagca aaaagctgaa
     3061 agccggcctg aaaccccaaa gcaaaagagt gaaggccggc ctgaaacccc aaaacagaag
     3121 ggtgatggac gacctgagac tccaaagcaa aagagtgaag gccggcctga aactccaaag
     3181 caaaaaggtg aaggccggcc tgaaactcca aaacataggc atgaaaatag gagggattct
     3241 ggaaagccat caacagagaa gaaacccgat gtgtctaagc ataaacagga tatcaaatct
     3301 gactcacctc gattaaaatc agaaagagct gaagccttaa agcagagacc tgatgggcga
     3361 tgggagtcac taagacgtga ccatgatagt aaacagaaat cagatgacag gggtgagtca
     3421 gagcgacatc ggggtgatca gtcaagggtt cgaagaccag aaacattgag atcttctagt
     3481 agaaatgacc atagcactaa atcagatggt tcaaaaactg aaaagctaga aagaaaacac
     3541 aggcatgaat caggggactc acgggacaga ccttctgggg aacaaaaatc aagaccagac
     3601 agtcctcgtg taaaacaagg agatactaat aaatcaagac ctggttttaa gtcaccaaat
     3661 agtaaagatg acaaaaggac agagggtaac agaagtaaag tagacagtaa taaagcgcac
     3721 actgataata aggccgaatt tccaagctat ttgttggggg ggaggtctgg tgcattgaaa
     3781 aatttcgtca ttccaaaaat caagagggat aaagatggca acataactca ggaaactaag
     3841 aaaatggaca tgaaaggaga gcagaaagac aaagtagaaa aaatgggatt agttgaagat
     3901 ctaaacaaag gagctaagcc tgtagttgtt ctgcaaaaac tatcgctgga tgatgtccag
     3961 aagctaatta aagatagaga ggagaaatca agaagttccc ttaagtctat caagaataag
     4021 ccatcaaagt caaataaagg tagtatagat caatcagtgt taaaagaatt gccccctgag
     4081 ctcctggcag aaattgaatc taccatgcca ctttgtgaac gtgtgaaaat gaacaaacgc
     4141 aagcgtagca cagttaatga aaagccaaaa tatgctgaaa tcagttcaga tgaagataat
     4201 gatagtgatg aagcgtttga atcctctagg aaacggcata aaaaagatga cgataaagct
     4261 tgggaatacg aagagcgcga cagaagaagc tctggggatc ataggagaag tggccactcc
     4321 catgacggaa ggaggagctc gggtggtggt cgttaccgaa accgaagtcc gtccgattct
     4381 gacatggaag attactcccc tcctcccagc cttagtgagg ttgctagaaa aatgaagaaa
     4441 aaagaaaaac aaaagaaaag gaaagcttac gagccaaagc taacgcccga agaaatgatg
     4501 gattcttcaa cttttaagag atttacagcc tcaatagaga atattttgga taatttagaa
     4561 gatatggatt ttactgcatt tggtgatgac gatgagattc ctcaggaact gcttttagga
     4621 aaacatcagc ttaatgaact tgggagtgaa tctgctaaaa ttaaagccat gggtataatg
     4681 gacaagcttt caactgacaa aactgtgaaa gtcctaaata tattggagaa gaatattcag
     4741 gatggatcaa agctttccac tttgttaaat cataataatg atactgaaga agaagaaaga
     4801 ttgtggagag acctaattat ggaaagagtt acaaagtcag cagatgcctg tcttacaact
     4861 atcaacatta tgacatctcc taacatgcca aaagctgtat acattgaaga tgtaattgaa
     4921 agggttatac agtacactaa gtttcattta cagaacacac tctatcccca gtatgaccct
     4981 gtttacagag tagatccaca tggaggaggg ttattaagtt caaaggccaa acgggctaaa
     5041 tgttctactc ataagcagag agtaattgta atgctttaca acaaagtttg tgacattgtt
     5101 agcagtttat cagaattgct agagatacag cttcttacag acacaacaat tcttcaggtt
     5161 tcatcaatgg gaataacacc cttttttgtg gaaaatgtca gtgaactaca attgtgtgcc
     5221 attaagttag tcactgcagt attttcaaga tatgaaaagc ataggcagtt aatattggaa
     5281 gaaattttta cttcacttgc aagattacca actagcaaga gaagtttaag gaatttcagg
     5341 ttaaatagta gtgatgtgga tggagaacct atgtatattc agatggttac agctctagtt
     5401 ttacaactta ttcagtgcgt agttcactta ccatcatcgg agaaggatcc taattcagaa
     5461 gaagactcaa acaaaaaagt tgatcaggat gttgtcatta cgaactctta cgaaacagct
     5521 atgagaacag cccaaaactt tctctccatc tttcttaaaa aatgtggtag taaacaagga
     5581 gaagaagatt acagaccgct ttttgaaaac tttgttcaag accttctctc aacagtcaat
     5641 aagcccgagt ggccggctgc tgaactactc cttagcttgt tagggaggct gttggttcat
     5701 cagttcagta acaagtcaac agaaatggct ttaagagtgg catctcttga ttaccttggg
     5761 actgttgctg cgcgactgag aaaagatgct gtcaccagca agatggatca agggtctata
     5821 gaacgaattt taaaacaggt ttctggagga gaagatgaga tccaacaatt gcaaaaagca
     5881 ttgcttgatt atctggatga gaacactgaa actgaccctt cgctagtgtt ttctcgtaaa
     5941 ttctatatag cccagtggtt ccgtgacaca actctggaaa cagaaaaagc aatgaaatca
     6001 caaaaagatg aagaatcatc tgacgcaaca catcatgcaa aagaacttga gacaactggg
     6061 cagatcatgc atcgcgctga gaaccggaaa aagtttcttc gaagcattat caaaaccaca
     6121 ccttctcagt tcagcacatt aaagatgaac tctgacactg tggactatga tgatgcgtgc
     6181 ttgattgttc gatatttggc ctctatgagg ccgtttgccc agagctttga tatttatttg
     6241 acacagatac tacgagttct tggtgaaaat gcaattgccg ttcgaacaaa agccatgaag
     6301 tgtttgtctg aggttgttgc tgtagacccc agtattctag caaggcttga catgcagcgc
     6361 ggagttcatg ggcgactaat ggataactcc actagtgttc gagaggcagc agtggaattg
     6421 ctaggtcgct ttgtcctgtg tcgccctcag cttgctgagc agtattatga catgctgatt
     6481 gaaagaatat tggatactgg gatcagtgtc aggaaaagag taataaagat tttaagagac
     6541 atctgtattg aacaaccaac atttccaaaa attacagaaa tgtgtgtaaa aatgattcgt
     6601 agagtcaatg atgaagaggg cattaagaaa ttagtaaatg aaacattcca aaagctctgg
     6661 tttactccaa ctccacataa tgacaaagaa gcaatgacaa ggaagatttt aaacatcact
     6721 gatgtggttg cagcatgtag agatactgga tatgactggt ttgagcaact ccttcaaaat
     6781 ttgttgaagt ctgaagagga ttcctcatat aaacctgtga agaaagcttg tactcaactt
     6841 gttgataacc tagttgaaca tattcttaaa tatgaggaat ctctagctga ctcagacaat
     6901 aaaggtgtaa attctggaag attggtagct tgtataacca ctttgttctt attcagcaaa
     6961 ataagacccc aactcatggt taaacatgcc atgactatgc agccgtacct caccactaag
     7021 tgtagtacgc aaaatgattt catggttatc tgcaatgttg caaaaatcct tgagttagtt
     7081 gtaccactga tggaacaccc aagtgaaact ttccttgcca ctattgagga agatctgatg
     7141 aagctgatca tcaaatacgg catgactgta gtacaacatt gtgtgagctg tcttggggct
     7201 gttgtaaata aagtgaccca gaattttaaa tttgtgtggg cctgtttcaa tagatactat
     7261 ggtgccattt caaaattaaa aagtcagcac caagaagacc caaataacac ttcactttta
     7321 acaaataaac cagcgcttct taggtccctc ttcactgttg gagcactttg ccgccatttt
     7381 gattttgatc tggaagattt taaaggcaac agtaaggtta acataaagga taaagtactt
     7441 gaactgttga tgtattttac aaaacactca gatgaagaag tacaaacaaa agccatcatt
     7501 ggtctaggat ttgcctttat acagcatccg agtctaatgt ttgagcaaga agtgaagaat
     7561 ctgtataata gtattttatc tgataagaac tcctcagtga atttaaaaat acaagtgtta
     7621 aaaaacctcc agacttacct acaagaagaa gacacgcgaa tgcaacaagc agacagagac
     7681 tggaagaaag ttgcaaaaca ggaagacctg aaggaaatgg gggacgtctc ctcggggatg
     7741 agcagctcca tcatgcagct gtacctcaag caggtgctgg aggcgttctt tcatacccag
     7801 tcaagtgtgc gccacttcgc cctgaatgtc attgctctga ctctgaacca aggcctcatc
     7861 catccggttc agtgtgttcc atacttaatt gccatgggaa cagatccaga acctgctatg
     7921 cgaaacaagg ctgatcagca gcttgtggag atagacaaaa aatatgctgg gttcatccat
     7981 atgaaagccg tggctggtat gaagatgtct taccaggtcc aacaggcgat caacacgtgc
     8041 ctaaaagacc ctgtaagggg tttccgacaa gatgaatcct ccagtgctct gtgctcacat
     8101 ctatactcca tgatccgcgg gaaccgccaa cacagacgcg cctttcttat ttctttactc
     8161 aacctctttg atgacacagc aaaaacagaa gtgactatgc tcttgtacat agcagacaat
     8221 ctagcctgtt ttccatacca gactcaggaa gagccgctgt ttataatgca ccacatagac
     8281 attacgctct ctgtttccgg tagtaaccta ttgcagtcat tcaaggagtc tatggtaaaa
     8341 gacaaaagga aagagaggaa aacgtcccct gctaaagaga acgagtccag tgagagtgaa
     8401 gaagaagtgt ccagacctcg gaagtctcga aagcgggtag attcggagtc agactcagac
     8461 tctgaagatg atataaattc agtgatgaag tgtttgccgg aaaattcagc ccctttgatt
     8521 gaattcgcaa atgtttccca gggtatttta ttacttctta tgttaaagca gcacctgaag
     8581 aatctctgtg gattttctga tagtaaaatt caaaaatatt ctccatctga atctgcaaaa
     8641 gtgtatgata aagccattaa tcggaaaaca ggggttcatt ttcatcccaa acaaacactg
     8701 gacttcctgc gaagtgacat ggctaattcc aaactgacgg aggatgtgaa gaggagcatc
     8761 gtaaggcagt acttagactt taaacttctc atggaacatc tagaccctga tgaagaagaa
     8821 gaggaaggag aggtgtcagc tagcaccaat gctcggaaca aagcaattac ctcgctgctt
     8881 ggaggaggca gccctaaaaa taacacagca gcagacacag aagatgagga gagtgatggg
     8941 gaggatagag gaggaggcac ttcagggtca ttgagaaggt caaaacgaaa ttcagactct
     9001 acggagttgg cagcacagat gaatgaaagt gttgacgtca tggatgtcat cgctatttgc
     9061 tgtccaaagt acaaagaccg accacagatt gcaagagtag tacagagaac cagcagtggc
     9121 gtcagtgtcc agtggatggc aggctcctac agcggctcct ggacggaggc caagcgccgc
     9181 gatggccgca aactggtgcc ttgggtagac actattaaag agtcagacat tatttacaaa
     9241 aaaattgctc taacgagtgc taataagctg actaataaag ttgttcagac tttacgatcc
     9301 ctgtatgccg ccaaggatgg gacttccagc taagtcgaaa cttgtttatt gcagcttata
     9361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9421 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     9481 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9541 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9601 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9661 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9721 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9781 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9841 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9901 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10021 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
    10081 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10141 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10201 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10261 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10321 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10381 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
    10441 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
    10501 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
    10561 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
    10621 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
    10681 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
    10741 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
    10801 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
    10861 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
    10921 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
    10981 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    11041 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    11101 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    11161 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    11221 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
    11281 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    11341 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    11401 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
    11461 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
    11521 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
    11581 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
    11641 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
    11701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    12001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    12061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    12121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12301 gggtcggaac aggagagcgc acgagggagc ttccagggg
//
